Protein Sequence Determination and FAB Mass Spectrometry
From the beginning, FAB (fast atom bombardment) mass spectrometry has held great promise for the structural analysis of peptides and proteins 1, 2. As the technique matures, it is evident that it will play an increasingly important role in primary structure determinations3. The purpose of this report is to define areas where FAB can be used to advantage for protein sequence determination with emphasis on the complimentary nature of this mass spectral technique with respect to more classical methods such as amino acid analysis and Edman microsequencing. The ability of FAB to analyze peptides directly without prior derivatization has facilitated the incorporation of this technique into protein sequencing strategies.
KeywordsGlycerol Adduct Angiotensin Disulfide Tryptophan
Unable to display preview. Download preview PDF.
- 3.T.D. Lee, Fast atom bombardment and secondary ion mass spectrometry of peptides and proteins, in: “Microcharacterization of Peptides and Proteins,” J.E. Shively, ed., The Humana Press, New Jersey, in press.Google Scholar
- 6.B.S. Rothman, D.H. Hawke, R.O. Brown, T.D. Lee, A.A. Dehghan, J.E. Shively and E. Mayeri, Isolation and primary structure of the califins, three biologically active, ELH-like peptides from the atrial gland of Aplysia californica, J. Biol. Chem., in press.Google Scholar
- 7.T.D. Lee, K. Legesse, D.H. Hawke, J.E. Shively, B.S. Rothman and E. Mayeri, Routine fast atom bombardment mass spectral analysis of high molecular weight peptides - atrial gland peptides from Aplysia californica, Bioc. Biop. Res. Commun., in press.Google Scholar
- 11.S. Rahbar, J. Louis, T.D. Lee and Y. Asmerom, Hemoglobin North Chicago: a new high affinity hemoglobin, Hemoglobin, in press.Google Scholar
- 12.S. Rahbar, T.D. Lee, J.A. Baker, L.T. Rabinowitz, Y. Asmerom and H.M. Ranney, Reverse phase high-performance liquid chromatography and secondary ion mass spectrometry. A strategy for identification of ten human hemoglobin variants, Hemoglobin, submitted.Google Scholar
- 15.C.M. Ben-Avram, T.D. Lee, R.C. LeBoeuf and J.E. Shively, The primary structure of murine apolipoprotein A-II from inbred mouse strain BALB/c, J. Biol. Chem., submitted.Google Scholar
- 17.T. D. Lee and V. Spayth, Computer assisted interpretation of fast atom bombardment mass spectra of peptides, 33rd Annual Conf. Mass Spectrom. Allied Topics, 266 (1985).Google Scholar