Abstract
A liquid chromatographic approach to automatic amino acid analysis first became available to analytical laboratories in 19581. Automated ion-exchange chromatography as developed by Moore, Stein, and Spackman has undergone refinements in the design of narrower bore columns, more sensitive flow cells, smaller resin particle size, and greater electronic amplification of spectroscopic signals. Improvements in the instrumentation design have made it possible to increase the sensitivity in the subnanomole range and decrease the analysis time from days to minutes2.
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© 1987 Plenum Press, New York
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Yang, Cy., Sepulveda, F.I., Yang, T., Huang, Wy. (1987). Amino Acid Analysis by Reverse-Phase High Performance Liquid Chromatography: Separation of Phenylthiocarbamyl Amino Acids by Spherisorb Octadecylsilane Columns. In: L’Italien, J.J. (eds) Proteins. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1787-6_25
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DOI: https://doi.org/10.1007/978-1-4613-1787-6_25
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