Mammalian Cell Expression and Characterization of Recombinant Human Antithrombin III

  • G. Zettlmeissl
  • H. Karges
  • U. Eberhard
Chapter
Part of the Developments in Hematology and Immunology book series (DIHI, volume 21)

Abstract

Antithrombin III (AT III) is one of the primary inhibitors of hemostasis [1]. By binding to and inhibiting thrombin, as well as several other activated clotting components (most notably Factors IXa, XIa and XIIa) AT III indirectly influences fibrin clot formation [1]. AT III makes a stochiometric 1:1 complex with thrombin. The complex building rate is increased by the binding of heparin by two orders of magnitude [2]. The physiological importance of AT III in preventing excessive coagulation is demonstrated by studies of individuals whose AT III levels are decreased due to hereditary [3–6] or acquired [7–9] deficiency. Human plasma AT III is a single chain glycoprotein of 432 residues containing three intramolecular disulphide bridges and four N-linked carbohydrate chains [10,11].

Keywords

Fermentation Carbohydrate Cysteine Heparin Electrophoresis 

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Copyright information

© Kluwer Academic Publishers, Boston 1988

Authors and Affiliations

  • G. Zettlmeissl
  • H. Karges
  • U. Eberhard

There are no affiliations available

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