Mammalian Cell Expression and Characterization of Recombinant Human Antithrombin III
Antithrombin III (AT III) is one of the primary inhibitors of hemostasis . By binding to and inhibiting thrombin, as well as several other activated clotting components (most notably Factors IXa, XIa and XIIa) AT III indirectly influences fibrin clot formation . AT III makes a stochiometric 1:1 complex with thrombin. The complex building rate is increased by the binding of heparin by two orders of magnitude . The physiological importance of AT III in preventing excessive coagulation is demonstrated by studies of individuals whose AT III levels are decreased due to hereditary [3–6] or acquired [7–9] deficiency. Human plasma AT III is a single chain glycoprotein of 432 residues containing three intramolecular disulphide bridges and four N-linked carbohydrate chains [10,11].
KeywordsFermentation Carbohydrate Cysteine Heparin Electrophoresis
Unable to display preview. Download preview PDF.
- 1.Rosenberg RD. Antithrombin III, biochemistry, function, assay and clinical significance. Ann Univ Sarav Med 1983;3:13–4.Google Scholar
- 24.Ouchterlony O. Progr Allergy 1958;5:1–78.Google Scholar
- 25.Hensen A, Loeliger EA. Antithrombin III assay. Thromb Diath Haemorrh 1963;9(suppl l):18–29.Google Scholar
- 27.Schrader J, Züchner C, Köstering H, Scheler F. Chromogenic substrates for the determination of antithrombin III. Ärztl Lab 1986;32:111-4.Google Scholar
- 28.Zettlmeissl G, Karges H, Eberhard U. Characterization of recombinant human antithrombin III from mammalian cells. (Unpublished data.)Google Scholar