Abstract
The mechanisms by which oestrogens promote and stimulate the growth of a significant percentage of breast cancers are still poorly understood (1–4 and refs. therein), although the basis for the hormonal dependence is clearly the presence of the oestrogen receptor. The MCF-7 cell line, which is derived from a pleural effusion of a breast cancer, contains both oestrogen and progesterone receptors (5–8 and refs therein). The levels of a variety of mRNAs, proteins and secreted growth factors are under oestrogen control in MCF-7 cells (3, 4, 9 and refs therein). As such, these cells represent a good in vitro model to study the molecular mechanisms underlying oestrogen action in breast cancers. To elucidate these mechanisms, we have undertaken several years ago a study aimed at characterizing genes whose expression is controlled by oestrogen in these cells and at cloning the human oestrogen receptor (hER) cDNA. We will summarize here our analysis of one such induced gene, named pS2, and the characterization of the protein for which it codes. We will also describe how the cloning and analysis of the hER cDNA has provided information concerning the mechanism by which the receptor activates the transcription of this and other oestrogen-responsive genes.
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© 1988 Kluwer Academic Publishers, Boston
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Chambon, P. et al. (1988). The Oestrogen-Induced pS2 Gene and the Oestrogen Receptor in Breast Cancer. In: Rich, M.A., Hager, J.C., Lopez, D.M. (eds) Breast Cancer: Scientific and Clinical Progress. Developments in Oncology, vol 56. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1753-1_5
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DOI: https://doi.org/10.1007/978-1-4613-1753-1_5
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