Abstract
A quantitative immunoprecipitation assay was conducted to study the developmental expression of nerve growth factor (NGF) receptor by using a monoclonal antibody (192-IgG) against rat NGF receptor. Either homogenates or plasma membrane enriched preparations from isolated tissues or whole rat embryos were bound with 125I-NGF and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. The receptor was then solubilized by 2% octylglucoside, bound with 192-IgG, and precipitated by secondary antibodies against mouse IgG prebound to Pansorbin. The complex, 125I-NGF/receptor/antibodies/Pansorbin, was separated from free ligand by rapid centrifugation through a 0.15 M sucrose cushion. The tube was frozen, and the tip containing the radioactivity was cut and counted. In some cases, the pellet was resuspended in SDS-PAGE sample buffer and subjected to a SDS-PAGE autoradiographic analysis.
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsEditor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Kluwer Academic Publishers
About this chapter
Cite this chapter
Yan, Q., Johnson, E.M. (1988). A Quantitive Study of the Developmental Expression of Nerve Growth Factor Receptors in Rats. In: Ferrendelli, J.A., Collins, R.C., Johnson, E.M. (eds) Neurobiology of Amino Acids, Peptides and Trophic Factors. Topics in the Neurosciences, vol 8. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1721-0_27
Download citation
DOI: https://doi.org/10.1007/978-1-4613-1721-0_27
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-8969-2
Online ISBN: 978-1-4613-1721-0
eBook Packages: Springer Book Archive