Abstract
The N-methyl-D-aspartate (NMDA) receptor is the best defined subclass of excitatory amino acid receptors with regard to both its electrophysiological properties and the functional consequences of its stimulation and inhibition. However, straightforward radioligand binding assays that specifically label the NMDA receptor are lacking. Specific binding of the NMDA selective antagonist D,L-[3H]-2-amino-7-phosphonoheptanoate has been demonstrated, but the pharmacological profile of this site does not correspond to the NMDA receptor [1]. The NMDA specific antagonist D-[3H]-2-amino-5-phosphono-pentanoate does label a recognition site that shows the pharmacological characteristic expected of the NMDA receptor [2]. However, the high ratio of nonspecific to specific binding with this ligand makes it less than ideal for receptor binding studies. The nonselective ligand L-[3H]glutamate has been used to label NMDA receptors. A study using postsynaptic densities demonstrated that 58% of the specific L-[3H]glutamate binding was displaceable by NMDA [3]. Recently, a report has appeared that compared the pharmacological profile of L-[3H]glutamate binding to an NMDA site in brain slices using autoradiography and in homogenates where the majority of the binding was displaceable by NMDA [4].
Keywords
- NMDA Receptor
- Postsynaptic Density
- Excitatory Amino Acid Receptor
- Synaptic Plasma Membrane
- Glutamate Binding
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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© 1988 Kluwer Academic Publishers
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Monahan, J.B., Michel, J., Hood, W.F., Pullan, L.M., Compton, R.P. (1988). L-[3H]Glutamate Binding to an N-Methyl-D-Aspartate Recognition Site in Synaptic Plasma Membranes. In: Ferrendelli, J.A., Collins, R.C., Johnson, E.M. (eds) Neurobiology of Amino Acids, Peptides and Trophic Factors. Topics in the Neurosciences, vol 8. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1721-0_20
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DOI: https://doi.org/10.1007/978-1-4613-1721-0_20
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