Specific (D, L)-[3H]2-Amino-4-Phosphonobutyric Acid Binding to Frozen Synaptic Plasma Membranes
In pathways where acidic amino acids, such as glutamate, are thought to be the endogenous neurotransmitter, electrophysiological studies have demonstrated that L-2-amino-4-phosphonobutyrate (L-APB) is a potent antagonist of the excitatory responses [1–3]. The binding of (D, L)-[3H]APB to its putative receptor can be readily differentiated from the binding to other subclasses of glutamate receptors by its dependence upon chloride. Furthermore, binding of APB to its recognition site has been reported to be sensitive to freezing. .
KeywordsSucrose EDTA Propionate NMDA Methylisoxazole
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