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Mass Spectrometry of Hexosamine Containing Oligosaccharides as Permethylated N-Trifluoroacetyl Derivatives

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The Molecular Immunology of Complex Carbohydrates

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 228))

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Abstract

Structural analysis of carbohydrates often includes mass spectrometry of derivatized monosaccharides or oligosaccharides. Mass spectrometry gives, combined with chemical analyses and NMR spectrometry, the complete structure. The major advantage with mass spectrometry is that structural information can be obtained on micro grams of material.

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References

  1. Nilsson, B. and Svensson, S. (1978). A New Method for N-deacetylation of 2-acetamido-2-deoxy Sugars. Carbohydr. Res. 62: 377–380.

    Article  CAS  Google Scholar 

  2. Nilsson, B. and Svensson, S. (1979). Studies on the Reactivity of Methyl Glycosides, Oligosaccharides and Polysaccharides towards Trifluoroacetolysis. Carbohydr. Res. 69: 292–296.

    Article  CAS  Google Scholar 

  3. Lindberg, B., Nilsson, B., Norberg, T. and Svensson, S. (1979). Specific Cleavage of O-glycosidic Bonds to L-serine and L-threonine by Trifluoroacetolysis. Acta Chem. Scand. B33: 230–231.

    Article  CAS  Google Scholar 

  4. Nilsson, B. and Svensson, S. (1979). A New Method for Degradation of the Protein Part of Glycoproteins; Isolation of the Carbohydrate Chains of Asialo Fetuin. Carbohydr. Res. 72.: 183–190.

    Article  PubMed  CAS  Google Scholar 

  5. Nilsson, B. and Zopf, D. (1983). Oligosaccharides Released from Glycolipids by Trifluoroacetolysis can be Analyzed by Gas Chromatography/Mass Spectrometry. Arch. Biochem. Biophys. 222: 628–648.

    Article  PubMed  CAS  Google Scholar 

  6. Hakomori, S. (1964). Rapid Permethylation of Glyco lipids and Polysaccharides, Catalyzed by Methylsulfinyl Carbanion in Dimethylsulfoxide. J. Biochem (Tokyo) 55: 205–208.

    CAS  Google Scholar 

  7. Nilsson, B. and Zopf, D. (1982). Gas Chromatography and Mass Spectrometry of Hexosamine Containing Oligosaccharide Alditols as their Permethylated N-trifluoroacetyl Derivatives. Methods in Enzymology. V. Ginsburg ed., Academic Press, New York, Vol. 83: 46–58.

    Google Scholar 

  8. Nilsson, B., Horne, M. and Gralnick, H. (1983). The Carbohydrate of Human Thrombin: Structural Analysis of Glycoprotein Oligosaccharides by Mass Spectrometry. Arch. Biochem. Biophys. 224: 127–133.

    Article  PubMed  CAS  Google Scholar 

  9. Nilsson, B., De Luca, S., Lohmander, S. and Hascall, V. (1982). Structures of N-linked and O-linked Oligosaccharides on Proteoglycan Monomer Isolated from the Swarm Rat Chondrosarcoma. J. Biol. Chem. 257: 10920–10927.

    PubMed  CAS  Google Scholar 

  10. Wu, A., Kabat, E.,Nilsson, B. and Zopf, D.,Gruezo, F. and Liao, J. (1984). Immunochemical Studies on Blood Groups. J. Biol. Chem. 259: 7178–7186.

    PubMed  CAS  Google Scholar 

  11. Sabharwal, H., Nilsson, B., Chester, A., Sjoblad, S. and Lundblad, A. (1984). Blood Group Specific Oligosaccharides from Faeces of a Blood Group A Breastfed Infant. Mol. Immunology 21: 1105–1112.

    Article  CAS  Google Scholar 

  12. Strömberg, S., Lindh, F. and Nilsson, B. (1985). Fast Atom Bombardment Mass Spectrometry of Oligosaccharides as Permethylated N-trifluoroacetyl Derivatives. Proceeding of the 8th International Symposium on Glycoconjugates, Houston, Texas, September 8–13, 1985.

    Google Scholar 

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© 1988 Plenum Press, New York

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Nilsson, B. (1988). Mass Spectrometry of Hexosamine Containing Oligosaccharides as Permethylated N-Trifluoroacetyl Derivatives. In: Wu, A.M., Adams, L.G. (eds) The Molecular Immunology of Complex Carbohydrates. Advances in Experimental Medicine and Biology, vol 228. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1663-3_35

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  • DOI: https://doi.org/10.1007/978-1-4613-1663-3_35

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8923-4

  • Online ISBN: 978-1-4613-1663-3

  • eBook Packages: Springer Book Archive

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