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Analysis of Complex Carbohydrate Primary and Secondary Structure via Two-Dimensional Proton Nuclear Magnetic Resonance Spectroscopy

  • Theodore A. W. Koerner
  • Robert K. Yu
  • J. Neel Scarsdale
  • Peter C. Demou
  • James H. Prestegard
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 228)

Abstract

The growing interest in the biological function of cell surface complex carbohydrates has stimulated the constant search for new methods for analyzing their primary and secondary structures. To determine the primary structure of a complex carbohydrate, it is necessary to establish the composition and configuration of its sugar residues, and the sequence and linkage sites of its oligosaccharide chain. This information has traditionally been obtained by application of a combination of such procedures as compositional analysis by gas-liquid chromatography, mass spectrometry, permethylation studies, Smith degradation, partial acid or enzyme hydrolysis, optical rotation measurements, etc. However, these techniques are time-consuming, require elaborate derivatization of the substance, and access to many different instruments. Furthermore, these procedures do not afford secondary structural information (conformation) which is important in understanding the biological activities of these compounds.

Keywords

Nuclear Magnetic Resonance Cross Peak Nuclear Magnetic Resonance Spectroscopy Complex Carbohydrate Proton Nuclear Magnetic Resonance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Theodore A. W. Koerner
    • 1
  • Robert K. Yu
    • 1
  • J. Neel Scarsdale
    • 1
  • Peter C. Demou
    • 1
  • James H. Prestegard
    • 1
  1. 1.Departments of Neurology (School of Medicine) and ChemistryYale UniversityNew HavenUSA

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