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Structural Concepts of the Human Blood Group A, B, H, Lea, Leb, I and i Active Glycoproteins Purified from Human Ovarian Cyst Fluid

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The Molecular Immunology of Complex Carbohydrates

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 228))

Abstract

The chemical structures of the blood group A, B, H, Lea, Leb, I and i determinants in water soluble blood group substances isolated from human ovarian cyst fluid have been studied for over four decades and are well established (1–10). However, the internal structure of the carbohydrate moiety had not been approached until base was applied to cleave the o- glycosidic linkage between the GaAc of the carbohydrate moiety and Thr and Ser of the protein core (8,11–15). The proposed composite structure of the carbohydrate side chains of blood group A, B, H, Lea and Leb substances (5,16,17) and of precursor substances with I and i determinants (18) shown in Fig. 1 were inferred from the mechanism of the alkaline ß-elimination and peeling reactions, together with the structures of the oligosaccharide fragments isolated (16) . More evidence is needed to confirm these carbohydrate internal structures. ß-elimination of intact blood group substances Fig. 1 (A) Proposed composite oligosaccharide structure (2,16,19) showing the relationship of the various blood group determinants and genes involved. Asterisk denotes that A substances have not been examined for oligosaccharides in this region (17).

The author did some of this work in Dr. E. A. Rabat’s laboratory between 1976–1981 at the Department of Microbiology, the College of Physicians and Surgeons, Columbia University, New York, New York, (17, 22, 23) and thanks him for his kind guidance.

For abbreviations of lectins, see Table IV in this text. All of the glycoproteins used for structural studies were purified from human ovarian cyst fluid. Therefore, it is the source if no indication is given otherwise; blood group active glycoprotein = blood group active substance; cyst glycoprotein = human blood group active glycoprotein, purified from ovarian cyst fluid.

Previous carbohydrate “core” structure is equivalent to internal structure (core structure and branches) in this text; and the carbohydrate chains prepared by alkaline ß-elimination and borohydride reduction are expressed as either R-GalNAc-ol or R-GalNAcα1→Ser(Thr) where R represents carbohydrate residues, but not for the peeling degraded products.

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Abbreviations

GalNAc:

2-acetamido-2-deoxy-D-galactopyranose

Gal:

D-galactopyranose

Fuc or LFuc:

L-fucopyranose

GlcNAc:

2-acetamido-2-deoxy-D-glucopyranose

GalNAc-ol:

2-acetamido-2-deoxy-Dgalactitol

Gal-ol:

galactitol

GLC-MS:

gas-liquid chromatography-mass spectrometry

HPLC:

high pressure liquid chromatography

FAB:

fast atom bombardment

NOE:

nuclear Overhauser enchancement

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  1. Department of Veterinary Pathology, Texas A&M University, 77843, College Station, Texas, USA

    Albert M. Wu

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    Albert M. Wu  & L. Garry Adams  & 

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Wu, A.M. (1988). Structural Concepts of the Human Blood Group A, B, H, Lea, Leb, I and i Active Glycoproteins Purified from Human Ovarian Cyst Fluid. In: Wu, A.M., Adams, L.G. (eds) The Molecular Immunology of Complex Carbohydrates. Advances in Experimental Medicine and Biology, vol 228. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1663-3_14

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  • DOI: https://doi.org/10.1007/978-1-4613-1663-3_14

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