Carbohydrate Structures as ONCO-Developmental Antigens and Components of Receptor Systems

  • Ten Feizi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 228)


In this article I shall review our research activities which have been focused on the antigenicities and biological roles of carbohydrate structures of glycoproteins and glycolipids. I shall first discuss examples of carbohydrate structures which behave as onco-developmental antigens recognized by monoclonal antibodies. This will be followed by a discussion of the possible involvement of carbohydrates in the functions of growth factor receptors and in the interactions of embryonic cells. The last section will be concerned with observations on the carbohydrate receptors for an infective agent, Mycoplasma pneumoniae, and new clues to the triggering of autoantibodies following human infection with this agent.


Blood Group Mycoplasma Pneumoniae Carbohydrate Moiety Carbohydrate Structure Carbohydrate Chain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Barondes, S.H. (1984). Soluble lectins: A New class of extracellular proteins. Science 223:1259–1264.PubMedCrossRefGoogle Scholar
  2. Bird, J.M. and Kimber, S.J. (1984) Oligosaccharides containing fucose linked a(l-3) and a(l-4) to N-acetylglucosamine cause decompaction of mouse morulae. Devel. Biol. 104:449–460.CrossRefGoogle Scholar
  3. Carding, S.R., Thorpe, S.J., Thorpe, R. and Feizi, T. (1985).Google Scholar
  4. Transformation and growth related changes in levels of nuclear and cytoplasmic proteins antigenically related to mammalian 3-galactoside-binding lectin. Biochem. Biophys. Res. Commun. 127:680–686.Google Scholar
  5. Childs, R.A., Pennington, J., Uemura, K., Scudder, P., Goodfellow, P.N., Evans, M.J. and Feizi, T. (1983). High molecular weight glycoproteins are the major carriers of the carbohydrate differentiation antigens I, i and SSEA-1 of mouse teratocarcinoma cells. Biochem. J. 215:491–503.PubMedGoogle Scholar
  6. Childs, R.A., Gregoriou, M., Scudder, P., Thorpe, S.J., Rees, A.R. and Feizi, T. (1984). Blood group active carbohydrate chains on the receptor for epidermal growth factor of A431 cells. EMBO. J. 3:2227–2233.Google Scholar
  7. Davidsohn, I., Kovarik, S. and Lee, C.L. (1966) A, B, and 0 substances in gastrointestinal carcinoma. Arch. Path. 81:381–390.PubMedGoogle Scholar
  8. Feizi, T., Taylor-Robinson, D., Shields, M.D. and Carter, R.A. (1969). Production of cold agglutinins in rabbits immunized with human erythrocytes treated with Mycoplasma pneumoniae. Nature 222:1253–1256.PubMedCrossRefGoogle Scholar
  9. Feizi, T. (1981a). The blood group Ii system: a carbohydrate antigen system defined by naturally monoclonal or oligoclonal autoantibodies of man. Immunol. Commun. 10:127–156.PubMedGoogle Scholar
  10. Feizi, T. (1981b). Carbohydrate differentiation antigens. Trends in Biochemical Sciences 6:333–335.CrossRefGoogle Scholar
  11. Feizi, T. (1983). In: Fetal antigens and cancer. Ciba Foundation Symposium 96. p.216–221. Ed. D. Evered & J. Whelan, Pitman.Google Scholar
  12. Feizi, T., Gooi, H.C., Childs, R.A., Picard, J.K., Uemura, K., Loomes, L.M., Thorpe, S.J. and Hounsell, E.F. (1984). Tumour-associated and differentiation antigens on the carbohydrate moieties of mucin-type glycoproteins. Biochem. Soc. Transact. 12:591–596.Google Scholar
  13. Feizi, T. (1985). Demonstration by monoclonal antibodies that carbohydrate structures of glycoproteins and glycolipids are onco-developmental antigens. Nature 314:53–57.PubMedCrossRefGoogle Scholar
  14. Feizi, T., Rabat, E.A., Vicari, G., Anderson, B. and Marsh, W.L. (1971a). Immunochemical studies on blood groups. XLVII. The I antigen complex - Precursors in the A, B, H, Lea, and Leb blood group sys- tem-Hemagglutination inhibition studies. J. Exp. Med. 133:39–52.PubMedCrossRefGoogle Scholar
  15. Feizi, T., Kabat, E.A., Vicari, G., Anderson, B. and Marsh, W.L. (1971b). Immunochemical studies on blood groups XLIX. The I antigen complex: specificity differences among anti-I sera revealed by quantitative precipitin studies; partial structure of the I determinant specific for one anti-I serum. J. Immunol. 106:1578–1592.PubMedGoogle Scholar
  16. Feizi, T., Turberville, C., Westwood, J.H. (1975). Blood-group precursors and cancer-related antigens. Lancet, ii:391–393.Google Scholar
  17. Feizi, T. and Hadler, N. (1983). Autoantibodies and disease. In:Google Scholar
  18. Biochemical Aspects of Human Disease. Eds: Elkeles, R.S. and Tavill, A.S. Blackwell Scientific Publications, pp. 656–692.Google Scholar
  19. Feizi, T. and Childs, R.A. (1985). Carbohydrate structures of glycoproteins and glycolipids as differentiation antigens, tumour-associated antigens and components of receptor systems. Trends in Biochem. Sci. 10:24–29.Google Scholar
  20. Fenderson, B.A., Zehavi, U. and Hakomori, S-I. (1984). A multivalent lacto-N-fucopentaose III - Lysyllysine conjugate decompacts preimplantation-stage mouse embryos while the free oligosaccharide is ineffective. J. Exp. Med. 160: 1591–1596.PubMedCrossRefGoogle Scholar
  21. Fukuda, M.N., Fukuda, M. and Hakomori, S. (1979). Cell surface modification by endo- β-galactosidase. Change of blood group activities and release of oligosaccharides from glycoproteins and glycosphingolipids of human erythrocytes. J. Biol. Chem. 254:5458–5465.PubMedGoogle Scholar
  22. Fukuda, M., Dell, A. and Fukuda, M.N. (1984a). Structure of fetal lactosaminoglycan. The carbohydrate moiety of band 3 isolated from human umbilical cord erythrocytes. J. Biol. Chem. 259:4782–4791.PubMedGoogle Scholar
  23. Fukuda, M., Dell, A., Oates, J.E. and Fukuda, M.N. (1984b). Structure of branched lactosaminoglycan, the carbohydrate moiety of band 3 isolated from adult human erythrocytes. J. Biol. Chem. 259:8260–8273.PubMedGoogle Scholar
  24. Gooi, H.C., Feizi, T., Kapadia, A., Knowles, B.B., Solter, D. and Evans, M.J. (1981). Stage specific embryonic antigen SSEA-1 involves al-3 fucosylated Type 2 blood group chains. Nature 292:156–158.PubMedCrossRefGoogle Scholar
  25. Gooi, H.C., Hounsell, E.F., Lax, I., Kris, R.M., Libermann, T.A.,Google Scholar
  26. Schlessinger, J., Sato, J.D., Kawamoto, T., Mendelsohn, J. and Feizi, T. (1985). The carbohydrate specificities of the monoclonal antibodies 29.1, 455 and 3C1B12 to the epidermal growth factor receptor of A431 cells. Bioscience Reps.5:83–94.CrossRefGoogle Scholar
  27. Hakomori, S. (1981). Blood group ABH and Ii antigens of human erythrocytes: chemistry, polymorphism and their developmental change. Semin. Hematol. 18: 39–62.PubMedGoogle Scholar
  28. Hakomori, S. (1984). Tumor-associated carbohydrate antigens. Ann. Rev. Immunol. 2: 103–126.CrossRefGoogle Scholar
  29. Kabat, E.A. (1973). Immunochemical studies on the carbohydrate moiety of water soluble blood group A, B, H, Lea and Leb substances and their precursor I antigens. In: Carbohydrates in Solution. Advances in Chemistry Series 117. American Chemical Society Washington, 334–361.Google Scholar
  30. Kapadia, A., Feizi, T. and Evans, M.J. (1981). Changes in the expression and polarization of blood group I and i antigens in post-implant- ation embryos and teratocarcinomas of mouse associated with cell differentiation. Exp. Cell Res. 131:185–195.PubMedCrossRefGoogle Scholar
  31. Loomes, L.M., Uemura, K-I., Childs, R.A., Paulson, J.C., Rogers, G.N., Scudder, P.R., Michalski, J-C., Hounsell, E.F., Taylor-Robinson, D. and Feizi, T. (1984). Erythrocyte receptors for Mycoplasma pneumoniae are sialylated oligosaccharides of Ii antigen type. Nature 307:560–563.PubMedCrossRefGoogle Scholar
  32. Loomes, L.M., Uemura, K-I. and Feizi, T. (1985). Interaction of Mycoplasma pneumoniae with antigen erythrocyte glycolipids of I and i antigen types. Infect. Immun. 47:15–20.PubMedGoogle Scholar
  33. Magnani, J.L., Smith, D.F. and Ginsburg, V. (1980). Detection of ganglio- sides that bind cholera toxin: direct binding of 125I -labelled toxin to thin-layer chromatograms. Anal. Biochem. 109:399–402.PubMedCrossRefGoogle Scholar
  34. Marsh, W.L. (1961). Anti-i: a cold antibody defining the Ii relationship in human red cells. Brit. J. Haematol. 7:200–209.CrossRefGoogle Scholar
  35. Masamune, H., Kawasaki, H., Abe, S., Oyama, K. and Yamaguchi, Y. (1958). Molisch positive ucopolysaccharides of gastric cancers as compared with the corresponding components of gastric mucosa. Tohoku J. exp. Med. 68:81–91.Google Scholar
  36. Picard, J., Waldron, E.D. and Feizi, T. (1978). Changes in the expression of the blood group A, B, H, Lea and Leb antigens and the blood group precursor associated I(Ma) antigen in glycoprotein-rich extracts of gastric carcinomas. J. Clin. Lab. Immunol. 119–128.Google Scholar
  37. Rastan, S., Thorpe, S.J., Scudder, P., Brown, S., Gooi, H.C. and Feizi, T. (1985). Cell interactions in preimplantation embryos: evidence for involvement of saccharides of the poly-N-acetyllactosamine series. J. Embryol. Exp. Morph. 87:115–126.Google Scholar
  38. Solter, D. and Knowles, B.B. (1978). Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1). Proc. Natl. Acad. Sci. USA 75:5565–5569.PubMedCrossRefGoogle Scholar
  39. Tang, P.W., Gooi, H.C., Hardy, M., Lee, Y.C. and Feizi, T. (1985). Novel approach to the study of the antigenicities and receptor functions of carbohydrate chains of glycoproteins. Biochem. Biophys. Res. Commun. 132:474–480.PubMedCrossRefGoogle Scholar
  40. Watkins, W.M. (1980). Biochemistry and genetics of the ABO, Lewis and P blood group systems. In: Advances in Human Genetics. Vol. 10 (Edited by Harris, H. and Hirschhorn, K.) pp. 1–136, 379–385. Plenum Publishing Co.Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Ten Feizi
    • 1
  1. 1.Applied Immunochemistry Research GroupClinical Research CentreHarrowUK

Personalised recommendations