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Tissue Culture of Conifers Using Loblolly Pine as a Model

  • H. V. Amerson
  • L. J. FramptonJr.
  • R. L. Mott
  • P. C. Spaine
Part of the Basic Life Sciences book series (BLSC, volume 44)

Abstract

Loblolly pine (Pinus taeda) is a good model for conifer tissue culture, since studies include three methods of in vitro propagation, long-term field evaluations of tissue-culture plantlets, and the development of in vitro trait selection methods. Abbreviated protocols are outlined for the following: (a) organogenesis of adventitious shoots and roots; (b) shoot micropropagation via fascicular and axillary shoots obtained from juvenile, adolescent, and mature explants; and (c) embryogenesis via immature zygotic embryos. Regulatory features of these processes, especially adventitious organ induction and shoot and root elongation, are examined. Field data on four- to six-year height growth, morphological characteristics, and fusiform rust resistance of tissue-culture plantlets derived from cotyledon explants are summarized from multiple plantings. Histological and immunological studies on fusiform rust resistance, evaluated in vitro in loblolly pine embryos, are presented as examples of in vitro trait selection.

Keywords

Adventitious Shoot Cotyledon Explants Hypocotyl Explants Immature Zygotic Embryo Shoot Initiation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • H. V. Amerson
    • 1
  • L. J. FramptonJr.
    • 1
  • R. L. Mott
    • 1
  • P. C. Spaine
    • 1
  1. 1.Departments of Forestry and BotanyNorth Carolina State UniversityRaleighUSA

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