Abstract
The DNA of frog virus 3 (FV3) replicates in two stages. During the first stage, genome sized DNA is synthesized in the nucleus of infected cells. This is followed by a second stage of DNA replication in the cytoplasm where concatemeric viral DNA is synthesized. FV3 DNA is methylated in the cytoplasm by a virus-coded DNA-methylase. It has been shown that more than 20% cytosine residues are methylated at the 5-carbon position. FV3 DNA is completely resistant to cleavage by a restriction endonuclease HpaII. a methylation-sensitive enzyme that recognizes the sequence CCGG, while readily restricted by another restriction enzyme MspI (an isoschizomer of HpaII), suggesting that every cytosine residue in the sequence of CpG is methylated. A number of DNA-methylase negative mutants have been derived from FV3 to elucidate the structure and function of this enzyme. Detailed genetic and biochemical studies involving one such mutant have revealed that the FV3 DNA-methylase, unlike other animal DNA-methylases, requires at least two polypeptides for its catalytic activity.
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© 1990 Kluwer Academic Publishers
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Essani, K. (1990). The DNA-Methylase of Frog Virus 3. In: Darai, G. (eds) Molecular Biology of Iridoviruses. Developments in Molecular Virology, vol 10. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1615-2_7
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DOI: https://doi.org/10.1007/978-1-4613-1615-2_7
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