Summary
Reperfusion of isolated rabbit heart after 60 min of ischaemia resulted in poor recovery of mechanical function, release of reduced (GSH) and oxidized glutathione (GSSG), reduction of tissue GSH/GSSG ratio and shift of cellular thiol redox state toward oxidation, suggesting the occurrence of oxidative stress. Pretreatment of the isolated heart with propionyl-L-carnitine (10-7M) improved the functional recovery of the myocardium, reduced GSH and GSSG release and attenuated the accumulation of tissue GSSG. This effect was specific for propionyl-L-carnitine as L-carnitine and propionic acid did not modify myocardial damage.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Fritz JB: Carnitine and its role in fatty acid metabolism. Advances in Lipid Research I 285–334, 1963
Liedtke AJ, Nellis SH, Neely JR: Effects of excess free fatty acids on mechanical and metabolic function in normal and ischaemic myocardium in swine. Circ Res 43: 652–661, 1978
Schwartz A, Wood JM, Allen JC, Bornet BP, Entman ML, Goldstein MA, Sordahl LA, Sucki M, Lewis RM: Biochemical and morphologic correlates of cardiac ischemia. I Membrane Systems. Am J Cardiol 32: 46–61, 1973
Shug AJ, Thomsen JH, Folts JD, Bittar N, Klein MI, Koke JR, Huth PJ: Changes in tissue levels of carnitine and other metabolites during myocardial ischaemia and anoxia. Arch Biochem Biophys 187: 25–33, 1978
Liedtke AJ, Nellis SH, Whitesell LF: Effects of carnitine isomers on fatty acid metabolism in ischemic swine hearts. Circ Res 48: 859–866, 1981
Liedtke AJ, Nellis SH: Effect of carnitine in ischemic and fatty acid supplemented swine hearts. J Clin Invest 64: 440–447, 1979
Folts JD, Shug AL, Koke JR, Bittar N: Protection of the ischemic dog myocardium with carnitine. Am J Cardiol 41: 1209–1214, 1978
Saless MF, Nellis SH, Liedtke AJ: The effects of propionyl-L-carnitine taurine on cardiac performance in aerobic and ischemic myocardium. J Mol Cell Cardiol 20: 63–74, 1988
Paulson DJ, Schmidt MJ, Romens J, Shug AL: Metabolic and physiological differences between zeroflow and low-flow myocardial ischemia: Effects of L-acetylcarnitine. Basic Res Cardiol 79: 551–561, 1984
Paulson DJ, Traxler J, Schmidt M, Noonan J, Shug AL: Protection of the ischemic myocardium by L-propionylcarnitine: Effects on the recovery of cardiac output after ischaemia and reperfusion, carnitine transport, and fatty acid oxidation. Cardiovasc Res 20: 536–541, 1986
Hulsmann WC, Dubelaar ML, Lamera JMJ, Maccari F: Protection by acyl-carnitine and phenylmethylsulfonyl fluoride of rat heart subjected to ischemia and reperfusion. Biochim Biophys Acta 847: 62–66, 1985
Ferrari R, Cucchini F, Visioli O: The metabolic effects of L-carnitine in angina pectoris. Intern J Cardiol 5: 213–216, 1984
Thomsen JH, Shug AL, Yap VU, Patel AK, Karras TJ, De Felice SL: Improved pacing tolerance of the ischaemic human myocardium after administration of carnitine. Am J Cardiol 43: 300–306, 1979
Amenta F, Gaetani F, Lucreziotti R, Ramacci MT: L-carnitine long term administration and myocardial morphofunctional parameters in aged rat. 9th International Congress of Pharmacology, London, Juphar, n. 2984, 1984 (Abstr.)
Ferrari R, Ciampalini G, Agnoletti G, Cargnoni A, Ceconi C, Visioli O: Effect of L-carnitine derivatives on heart mitochondrial damage induced by lipid peroxidation. Pharmacol Res Comm 20: 125–132, 1988
Ferrari R, Ceconi C, Curello S, Guarnieri C, Caldarera CM, Albertini A, Visioli O: Oxygen mediated myocardial damage during ischaemia and reperfusion: role of the cellular defences against oxygen toxicity. J Mol Cell Cardiol 17: 937–945, 1985
Curello S, Ceconi C, Cargnoni A, Cornacchiari A, Ferrari R, Albertini A: Improved procedure for determining glutathione in plasma as an index of myocardial oxidative stress. Clin Chem 33/8: 1448–1449, 1987
Tietze F: Enzymatic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissue. Anal Biochem 27: 502–522, 1969
Sedlack J, Lindsay RH: Estimation of total, proteinbound, and non protein sulphydryl groups in tissue with Ellman’s reagent. Anal Biochem 25: 192–205, 1968
Paglia DE, Valentine WN: Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70: 158–169, 1967
Bradford MM: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle for protein binding. Anal Biochem 72: 248–254, 1976
Ferrari F, Cargnoni A, Ceconi C, Visioli O: Protective effect of L-propionyl carnitine against myocardial ischaemic and reperfusion damage. Eur Heart J 8: 86, 1987 (Abstr.)
Di Lisa F, Raddino R, Ferrari R: Modificazioni metaboliche e contrattili indotte da diversi substrati in corso di ischemia sperimentale e successiva riperfusione. Giornale di Clinica e Fisiopatologia Cardiovascolare 1: 65–75, 1982
Neely JR, Garber D, McDonoughK, Idell-Wenger J: Relationship between ventricular function and intermediates of fatty acid metabolism during myocardial ischaemia: effects of carnitine. In: MM Winbury and Y Abiko (eds) Ischemic myocardium and antianginal drugs. Raven Press, New York, 1979, pp 225–234
Hearse D, Shattock MJ, Manning AS, Braimbridge MV: Protection of the myocardium during ischaemic arrest: possible toxicity of carnitine in cardioplegic solutions. Thorac Cardiovasc Surg 28: 253–8, 1980
Paulson DJ, Shug AL: Effects of carnitine on the ischaemic arrested heart. Basic Res Cardiol 77: 460–3, 1982
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Kluwer Academic Publishers
About this chapter
Cite this chapter
Ferrari, R., Ceconi, C., Curello, S., Pasini, E., Visioli, O. (1989). Protective effect of propionyl-L-carnitine against ischaemia and reperfusion-damage. In: Van Der Vusse, G.J. (eds) Lipid Metabolism in Normoxic and Ischemic Heart. Developments in Molecular and Cellular Biochemistry, vol 5. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1611-4_23
Download citation
DOI: https://doi.org/10.1007/978-1-4613-1611-4_23
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-8891-6
Online ISBN: 978-1-4613-1611-4
eBook Packages: Springer Book Archive