Summary
The regulation of and the intracellular events following α1-aradrenergic receptor stimulation in the myocardium still remain to be disclosed. The effect of aradrenergic stimulation on phosphoinositide breakdown was studied in cultured neonatal rat ventricular myocytes. Phenylephrine (30 μM) stimulated inositolphosphates formation, but only in the presence of 10 mM LiCl this could be measured. The increase was antagonized by prazosin (1 μM) but not by propranolol (1 μM). The variability in proportional distribution of the three inositolphosphates is discussed.
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Abbreviations
- PIP2 :
-
Phosphatidylinositol 4,5-bisphosphate
- PI:
-
Phosphatidylinositol
- PI3 :
-
Inositol 1,4,5,-trisphosphate
- IP2 :
-
Inositol 1,4-bisphosphate
- IP1 :
-
Inositol 1-monophosphate
- DG:
-
Diacylglycerol
- PKC:
-
Calcium/phospholipid-dependent protein kinase
- PhE:
-
Phenylephrine
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© 1989 Kluwer Academic Publishers
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Meij, J.T.A., Lamers, J.M.J. (1989). Alpha-1-adrenergic stimulation of phosphoinositide breakdown in cultured neonatal rat ventricular myocytes. In: Van Der Vusse, G.J. (eds) Lipid Metabolism in Normoxic and Ischemic Heart. Developments in Molecular and Cellular Biochemistry, vol 5. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1611-4_11
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DOI: https://doi.org/10.1007/978-1-4613-1611-4_11
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