Oncogenes pp 25-36 | Cite as

Molecular biology: Concepts and techniques

  • Edison Liu
  • James W. Larrick
Part of the Cancer Treatment and Research book series (CTAR, volume 47)


This chapter briefly reviews the principles of molecular biology, and the techniques that frequently are mentioned in this volume. The nuances of molecular biology are not explored, and the discussions of certain biological processes are necessarily dogmatic. The reader is referred to more lengthy texts for detailed discussions [1–3].


Radioactive Probe Dideoxy Sequencing Western Blot Hybridization Sugar Backbone NIH3T3 Fibroblast Cell Line 
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  1. 1.
    Davis LG, Dibner MD, Batley JF: Basic Methods in Molecular Biology. New York, Elsevier, 1986.Google Scholar
  2. 2.
    Watson JD, Tooze J, Kurtz DT: Recombinant DNA: A Short Course. New York, Scientific American Books, W.H. Freeman 1983.Google Scholar
  3. 3.
    Berger SL, Kimmel AR (eds): Guide to Molecular Cloning Techniques: Methods in Enzy-mology, volume 152. New York, Academic Press, 1987.Google Scholar
  4. 4.
    Southern EM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:590–513, 1975.CrossRefGoogle Scholar
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    Saiki RK, Gelfand DH, Stoffel S, et al.: Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491, 1988.PubMedCrossRefGoogle Scholar
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    Mullis KB, Faloona F: Specific synthesis of DNA in vitro via a polymerase catalyzed chain reaction. Methods Enzymology 155:335–350, 1987.CrossRefGoogle Scholar
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    Wigler M, Pellicer A, Silverstein S, et al.: Biochemical transfer of single-copy eukaryotic genes using total cellular DNA as donor. Cell 14:725–734, 1978.PubMedCrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Edison Liu
  • James W. Larrick

There are no affiliations available

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