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Use of a Dot-Elisa Procedure for the Detection of Specific Antibodies in Cutaneous Leishmaniasis

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Leishmaniasis

Part of the book series: NATO ASI Series ((NSSA,volume 171))

Abstract

American Tegumentary Leishmaniasis is a disease endemic on the America continent from northern Mexico to Argentina. This disease is of major importance in Peru, with over 2700 new cases being reported annually to Peruvian Ministry of Health, a figure which most probably is an underestimate1.

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References

  1. H. Lumbreras and H. Guerra, Leishmaniasis in Peru, in “Leishmaniasis”, K-P Chang and R.S. Bray ed. Elsevier, Amsterdam (1985).

    Google Scholar 

  2. G. G. Romero, M. Arana, M. López, Y. Montoya, R. Bohl, M. Campos, J. Arévalo and A. Llanos, Characterization of Leishmania species from Peru, Trans., R. Soc. Trop. Med..; Hyg. 81:14 (1987).

    Article  CAS  Google Scholar 

  3. L. D. Hendricks and N. Wright, Diagnosis of cutaneous leishmaniasis by In vitro cultivation of saline aspirates in Schneider Drosophila medium, Amer. J. Trop. Med. Hyg., 28:962 (1979).

    CAS  Google Scholar 

  4. A. Herrer, V. E. Thatcher and C. M. Johnson, Natural infe£ tion of Leishmania and Trypanosomes demonstrated by skin culture, J. Parasitol, 52:954 (1966).

    Article  PubMed  CAS  Google Scholar 

  5. A. Herrer, H. A. Christensen and R. J. Beumer, Reservoir hosts of cutaneous leishmaniasis among Panamenian forest mammals, Am. J. Trop. Med. & Hyg. 22:585 (1973).

    CAS  Google Scholar 

  6. B. C. Walton, W. H. Brooks and J. Arjona, Serodiagnosis of American Leishmaniasis by indirect fluorescent antibody test, Am. J. Trop. Med. Hyg., 21:296 (1972).

    PubMed  CAS  Google Scholar 

  7. C. A. Cuba-Cuba, P. D. Marsden, A. C. Barreto, R. Rocha, R. R. Sampaio and L. Patzlaff, Diagnöstico parasitology co e inmunológico de Leishmania tegumentaria americana. Bol. Of. Pan.’Sal. 89:195 (1980).

    CAS  Google Scholar 

  8. M. G. Pappas, P. B. Mc Greevy, R. Hajkowski, L. D. Hendricks, C. N. Oster and W. T. Hockmeyer, Evaluation of promastigotes antigens in the indirect fluorescent anti body test for American cutaneous leishmaniasis, Am. J. Trop. Med. Hyg., 32:1260 (1983).

    PubMed  CAS  Google Scholar 

  9. M. Hommel, Enzymoimmunoassay in Leishmaniasis, Trans. R. Soc. Trop. Med. Hyg., 70:15 (1976).

    Google Scholar 

  10. M. Hommel, N. Peters, J. Ranque, M. Quilici and G. Lanotte, The micro-elisa technique in the serodiagnosis of visce ral leishmaniasis, Am. Trop. Med. Parasitol., 72:213 (1978).

    CAS  Google Scholar 

  11. M. G. Pappas, R. Hajkowski and W. T. Hockmeyer, Dot-Enzyme-linked immunosorbent assay (dot-Elisa): a micro technique for the rapid diagnosis of visceral leishmaniasis. J. Immunol. Methods, 64:205 (1983).

    Article  PubMed  CAS  Google Scholar 

  12. M. G. Pappas, R. Hajkowski and W. T. Hockmeyer, Standarization of the dot-enzyme-linked immunosorbent assay (dot-Elisa) for human visceral leishmaniasis, Am. J. Trop. Med. Hyg., 33:1105 (1984).

    PubMed  CAS  Google Scholar 

  13. M. G. Pappas, R. Hajkowski, D. B. Tang and W. T. Hockmeyer, Reduced false positive reactions in the dot-enzyme-linked immunosorbent assay for human visceral leishmaniasis. Clin. Immunol. Immunopathol., 34:392 (1985).

    Article  PubMed  CAS  Google Scholar 

  14. M. C. S. Guimaraes, B. J. Celeste, M. E. Camargo and J. M. P. Dimiz, Seroepidemiology of cutaneous leishmaniasis from riberia do Iguape Valley. Ig M and Ig G antibodies detected by means of an immunoenzymatic assay (ELISA), Rev. do Ins. Med. Trop. Sao Paulo, 25:99 (1983).

    Google Scholar 

  15. L. A. Guevara, L. T. Paz, F. E. Nieto and J. Arévalo, Improvement of a dot-Elisa, using alkaline phosphatase, for the detections of antibodies in mucocutaneous leish maniasis, (1987, submitted).

    Google Scholar 

  16. O. H. Lowry, N. J. Rosebrough, A. L. Farr and R. J. Randall, Protein measurement with Folin phenol reagent, J. Biol. Chem., 193:275 (1951).

    Google Scholar 

  17. P. Griner, J. Raymond, A. Mushlin and P. Greenland, Selection and interpretation of diagnostic test and proce-dures, Ann. Int. Med. 94:553 (1981).

    Google Scholar 

  18. R. E. Duxbury and E. H. Sadum, Fluorescent antibody test for the serodiagnosis of visceral Leishmaniasis, Am. J. Trop. Med. Hyg., 18:525 (1964).

    Google Scholar 

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Guevara, L., Paz, L., Nieto, E., Llanos, A. (1989). Use of a Dot-Elisa Procedure for the Detection of Specific Antibodies in Cutaneous Leishmaniasis. In: Hart, D.T. (eds) Leishmaniasis. NATO ASI Series, vol 171. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1575-9_43

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  • DOI: https://doi.org/10.1007/978-1-4613-1575-9_43

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8862-6

  • Online ISBN: 978-1-4613-1575-9

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