Flow Cytometric Analysis of Leishmania Surface Membrane Antigen Expression
The advent of cell fusion technology for the construction of hybridomas synthesizing monoclonal antibodies (Köhler and Milstein, 1975) has provided the immunoparasitologist with the means to detect subtle antigenic differences on the surface membrane of the leishmania parasites which cause human disease. Use of these antibodies in radioimmune binding assays (McMahon-Pratt and David, 1981; Jaffe and McMahon-Pratt 1983; Jaffe et al., 1984; Pan et al., 1984; McMahon-Pratt et al., 1985; Grimaldi et al., 1987), immunofluorescent antibody assays (Handman and Hocking, 1982; de Ibarra et al., 1982; Lemesre et al., 1985) and enzyme linked immunosorbant assays (Fong and Chang, 1982; Anthony et al., 1985) has now culminated in the identification of a sizeable composite of species-specific, subspecies-specific and stage-specific epitopes. Most importantly, the ability to demonstrate such epitopes on parasites taken directly from the patient1s lesion (Lynch et al., 1986; Anthony et al., 1987) permits a prompt diagnosis of the infection. Such diagnoses are critical when attempting to decide regimens of treatment, management of the patient and prognosis.
KeywordsBromide Capron Hepes Paraformaldehyde Ethidium
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- McMahon-Pratt, D., Bennett, E., Grimaldi, G., and Jaffe, C.L., 1985, Subspecies- and species-specific antigens of Leishmania mexicana characterized by monoclonal antibodies, J. Immunol., 134:1935.Google Scholar