Prolonged Storage of Adult and Newborn Red Cells

  • N. Meyerstein
Part of the Developments in Hematology and Immunology book series (DIHI, volume 24)


Prolonged storage of red blood cells induces structural and metabolic changes, known as “storage lesion” [1]. This causes impaired cellular function and a decreased survival time after transfusion [2]. Suitable preservation media may maintain ATP contents and improve in vivo survival [3]. Recently a new preservation medium was composed by Meryman et al, consisting of a hypotonic solution with respect to non permeant solutes [4]. Ammonium chloride, adenine, glucose, mannitol, citrate and phosphate were included in this medium [4]. Unexpectedly, this medium augmented ATP levels and maintained them above initial values for 9 weeks, as well as prolonged in vivo survival at acceptable values, for up to 18 weeks.


Ammonium Chloride Prolonged Storage Hypotonic Solution Adult Cell Osmotic Fragility 
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  1. 1.
    Schrier SL, Hardy B, Bensch KV, Junga I, Krueger R. Red blood cell membrane storage lesion. Transfusion 1979; 19: 158–65.PubMedCrossRefGoogle Scholar
  2. 2.
    Haradin AR, Weed RI, Reed CF. Changes in physical properties of stored erythrocytes. Transfusion 1969; 9: 229–37.PubMedCrossRefGoogle Scholar
  3. 3.
    Beutler E. Experimental blood preservatives for liquid storage. In: Greenwalt TJ, Jamieson GA (eds). The red cell in vitro. New York: Grune and Stratton, 1974: 189–217.Google Scholar
  4. 4.
    Meryman HT, Hornblower LS, Syring RL. Prolonged storage of red cells at 4°C. Transfusion 1986; 26: 500–5.PubMedCrossRefGoogle Scholar
  5. 5.
    Mazor D, Dvilansky A, Meyerstein N. Prologned storage of red cells with ammonium chloride and mannitol. Transfusion (in press)Google Scholar
  6. 6.
    Beutler E, Kuhl W. Volume of erythrocytes during storage. The role of mannitol. Transfusion 1988; 28: 353–7.PubMedCrossRefGoogle Scholar
  7. 7.
    Golstein S, Czapski G. Mannitol as an OH scavenger in aqueous solutions and in biological systems. Int J Radiat Biol 1984; 46: 725–9CrossRefGoogle Scholar
  8. 8.
    Meyerstein N, Mazor D, Dvilansky A. Changes in agglomeration of human red blood cells in liquid storage in CPD media. Transfusion 1977; 17: 115–22.PubMedCrossRefGoogle Scholar
  9. 9.
    Beutler E. In: Red cell metabolism. A manual of biochemical methods. 3rd edition. New York: Grune and Stratton 1984: 127–31.Google Scholar
  10. 10.
    Dacie JV, Lewis SM. Practical haematology. Churchill Livingstone 1984: 152–6.Google Scholar
  11. 11.
    Högman CF, Hedlung K, Sahlestrom Y. Red cell preservation in protein-poor media. III. Protection against in vitro hemolysis. Vox Sang 1981; 41: 274–81.CrossRefGoogle Scholar
  12. 12.
    Naiman J, Oski FA. Hematologic problems in the newborn. Vol 4, 3rd edition. Philadelphia: Saunders, Co. 1982: 1–30, 97–136.Google Scholar
  13. 13.
    Wolfe LC. The membrane and the lesions of storage in preserved red cells. Transfusion 1985; 25: 185–203.PubMedCrossRefGoogle Scholar
  14. 14.
    Horn S, Mazor D, Zmora E. Meyerstein N. Storage induced changes in human newborn red cells. Transfusion 1987; 27: 411–4.PubMedCrossRefGoogle Scholar
  15. 15.
    Lachant NA, Noble NA, Myrhe BA, Tanaka KR. Antioxidant metabolism during blood storage and its relationship to posttransfusion red cell survival. Am J Hematol 1984; 17: 237–49.PubMedCrossRefGoogle Scholar
  16. 16.
    Sjolin S. Resistance of red cells in vitro: study of osmotic properties, mechanical resistance and storage behaviour of red cells of fetuses, children and adults. Acta Paediatr 1954; 43 (Suppl. 98): 1–92.Google Scholar
  17. 17.
    Brandes JM, Roth EF, Berk PD et al. Collection and preservation of human placental blood. Transfusion 1983; 23: 325–7.PubMedCrossRefGoogle Scholar

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© Kluwer Academic Publishers, Boston 1990

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  • N. Meyerstein

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