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Cryopreservation of Human Stem Cells

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Cryopreservation and low temperature biology in blood transfusion

Part of the book series: Developments in Hematology and Immunology ((DIHI,volume 24))

Abstract

Autologous bone marrow transplantation (AMBT) is widely used to circumvent the myelotoxicity of intensive therapeutic regimens. Numerous clinical results provide evidence that autografting limits myelosuppression [1] and/or reconstitutes the host’s hemopoietic system after lethal marrow injury [2]. Studies in recent years have demonstrated that recovery of hemopoiesis can also be achieved by the infusion of peripheral blood stem cells [3]. Whatever the source of stem cells, i.e. bone marrow or peripheral blood, they usually have to be stored for long periods before transplantation. The success of engraftment depends on the dose and the functional integrity of the cells infused, i.e. the quality of the stem cell collection and the storage procedure. Since the initial observations of Barnes and Loutit in rodents, studies in a variety of animal species [4,5,6] have evaluated low-temperature storage of marrow cells for functional use. Following these data, the efficiency of human stem cell cryopreservation has been intensively documented [7,8,9].

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Authors
  1. F. Beaujean
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Editors and Affiliations

  1. Red Cross Blood Bank Groningen-Drenthe, The Netherlands

    C. Th. Smit Sibinga  & P. C. Das  & 

  2. Jerome H. Holland Laboratory, American Red Cross, Rockville, MD, USA

    H. T. Meryman

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© 1990 Kluwer Academic Publishers, Boston

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Beaujean, F. (1990). Cryopreservation of Human Stem Cells. In: Smit Sibinga, C.T., Das, P.C., Meryman, H.T. (eds) Cryopreservation and low temperature biology in blood transfusion. Developments in Hematology and Immunology, vol 24. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1515-5_18

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  • DOI: https://doi.org/10.1007/978-1-4613-1515-5_18

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8814-5

  • Online ISBN: 978-1-4613-1515-5

  • eBook Packages: Springer Book Archive

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