Abstract
The advantage of the ‘bifunctional chelate’ as an alternative to radioiodination for the labeling of antibodies and other proteins has been recognized and commented on for several years [1]. This recognition has led to the development of diverse chelators — a process that continues today [2]. Early on, interest focused on diethylenetriaminepentaacetic acid (DTPA) as a suitable chelator for this purpose, since the polyaminopolycarboxylic acids, of which DTPA is an example, form strong chelates with a large number of metallic radionuclides [3]. Prior to 1981, the mixed anhydride of DTPA was the agent of choice for conjugation of DTPA to proteins [4], but in that year this laboratory found that the cyclic anhydride of DTPA (cDTPA) could be used to attach DTPA to proteins by a simpler process, which offers a considerable degree of control over the conjugation [5]. As a result, cDTPA has been used extensively in this and other laboratories for the preparation of DTPA-conjugated antibodies and other proteins. For example, in addition to both polyclonal and monoclonal antibodies, other proteins have been conjugated, such as fibrinogen [6,7], albumin [5], tissue plasminogen activator [8], avidin and streptavidin [9], lysozyme [10], and others [10]. After conjugation these proteins have been labeled primarily with 111In but also with 90Y [11–14], 88Y [15], 99mTc [10,16], 153Gd [17–19], 109Pd [20], 46Sc, and 67Ga [21].
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Hnatowich, D.J. (1990). Radiolabeling antibodies via the cyclic anhydride of DTPA — Experiences of 5 years. In: Goldenberg, D.M. (eds) Cancer Imaging with Radiolabeled Antibodies. Cancer Treatment and Research, vol 51. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1497-4_8
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DOI: https://doi.org/10.1007/978-1-4613-1497-4_8
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