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Experimental model systems for antibody targeting and radioimmunodetection

  • Rosalyn D. Blumenthal
  • Robert M. Sharkey
  • David M. Goldenberg
Part of the Cancer Treatment and Research book series (CTAR, volume 51)

Abstract

The development of radiolabeled antibodies for clinical imaging and therapy has relied extensively on a variety of animal models. The design and use of animal models in radiotracer studies, including radiolabled antibodies, has been reviewed [1]. The first animal studies in the field of radioimmunodetection (RAID) were performed in the late 1940s and early 1950s. In a study to determine if anti-organ antibodies could localize specifically to the target organ, Pressman and Keighly [2] were the first to show localization of kidney following intravenous injection of I-131-labeled rabbit antibody against rat kidney. Subsequent studies confirmed that antibodies could be prepared with specificities against other organs, such as adrenals, ovary, lung, and small intestine [3]. Encouraged by these findings that organ-specific antibodies could be developed, investigations began to determine if tumor-specific antibodies could be generated. Pressman and Korngold [4] demonstrated selective tumor targeting in mice bearing the Wagner-osteogenic mouse sarcoma cell line. One of the earliest antigens used to localize humans tumors was fibrin [5], and I-131-labeled antibodies to fibrin were used in two patients for therapy [6]. However, it was recognized that more extensive investigations were needed to make this method useful for the early detection of tumors in humans.

Keywords

Nude Mouse Tumor Target Human Tumor Xenograft Cheek Pouch Hamster Cheek Pouch 
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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Rosalyn D. Blumenthal
  • Robert M. Sharkey
  • David M. Goldenberg

There are no affiliations available

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