The ultrastructure of calcified tissues: Methods and technical problems
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The organic/inorganic interface within calcified tissue matrices is both intriguing and problematic. It is intriguing because these specialized matrices initiate and control mineral deposition while serving as a means for storage and retrieval of various essential ions. Also, these matrices, at the cellular level, provide protection against the lethal stores of concentrated calcium phosphate in the form of microcrystalline apatite, Ca10(PO4)6(OH)2. The problematic aspect of calcified tissues is due to the complexity of this physico-chemical organic/inorganic interface, i.e., what is the exact nature of this interface, what chemical factors are involved with the initiation and control of mineral deposition, what factors govern crystal size and orientation, and what are the different phases of calcium phosphate other than apatite.
KeywordsCollagen Fibril Scanning Transmission Electron Microscopic Matrix Vesicle Apatite Crystal Calcify Cartilage
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