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Influence of glycosylation inhibitors on dihydropyridine binding to cardiac cells

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Biochemical Mechanisms in Heart Function

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 18))

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Abstract

In primary cultures of neonatal rat heart cells we found a linear correlation between the number of L-type calcium channel-specific dihydropyridine (DHP) binding sites and spontaneous beating frequency (v).

Formation of glycoproteins in tissue culture was suppressed by different inhibitors of N-glycosylation. This inhibition alters to a different extent the binding of the DHP ligand (+)-[methyl-3H]PN 200-110 and v. The most severe but reversible effect occurs at 6 µ/ml tunicamycin (Bmax ≈ 45% and v ≈ 6%, resp., of control), a slight increase in Bmax at 0.1–0.5 mM castanospermine and 0.05–2.5 mM deoxymannojirimycin. The other inhibitors gave no significant alterations of Bmax. (Mol Cell Biochem 160/161:45–52, 1996)

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Author information

Authors and Affiliations

  1. Institute of Biochemistry and Molecular Physiology, University of Potsdam, Robert-Rössle-Str. 10, D-13122, Berlin, Germany

    Ursula Henning & Martin Holtzhauer

  2. Department of Molecular Cardiology, Max Delbrück Center for Molecular Medicine, R-Rössle-Str. 10, D-13122, Berlin, Germany

    Gerd Wallukat

Authors
  1. Ursula Henning
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  2. Gerd Wallukat
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  3. Martin Holtzhauer
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Editor information

Editors and Affiliations

  1. Max Delbruck Center for Molecular Medicine, Robert-Rossle-Straβe 10, 13122, Berlin-Buch, Germany

    Ernst Georg Krause  & Roland Vetter  & 

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© 1996 Kluwer Academic Publishers

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Henning, U., Wallukat, G., Holtzhauer, M. (1996). Influence of glycosylation inhibitors on dihydropyridine binding to cardiac cells. In: Krause, E.G., Vetter, R. (eds) Biochemical Mechanisms in Heart Function. Developments in Molecular and Cellular Biochemistry, vol 18. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1279-6_6

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  • DOI: https://doi.org/10.1007/978-1-4613-1279-6_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8546-5

  • Online ISBN: 978-1-4613-1279-6

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