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Prevention by 7-oxo-prostacyclin of the calcium paradox in rat heart: Role of the sarcolemmal (Na,K)-ATPase

  • Attila Ziegelhöffer
  • Tatiana Ravingerová
  • Andrej Džurba
  • Narcisa Tribulová
  • Ján Slezák
  • Albert Breier
  • László Szekeres
Chapter
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 18)

Abstract

It is demonstrated a fast and significant depression in the sarcolemmal (Na,K)-ATPase activity that occurs as early as 25 sec after the onset of Ca2+ depletion, and participates in the development of Ca2+-paradox in the rat heart. Pretreatment of the animals with 7-oxo-prostacyclin (PGI2) 24–48 h prior to the experiment prevented fairly the Ca2+-depletion-induced depression in (Na,K)ATPase activity and the accompanying structural and functional damage to the heart and sarcolemma during Ca2+-depletion as well as the development of Ca2+-paradox during the subsequent Ca2+-repletion. Pretreatment with PGI2 was chosen intentionally because previous experiments revealed, that in its late effect the drug is acting via stabilizing the membranes due induction of high activity of (Na,K)-ATPase that has increased affinity to ATP. From results obtained the following may be concluded: If during the phase of Ca2+-deprivation, the capability of heart sarcolemma to maintain sodium extrusion remains preserved, the expected aggravation of Ca2+-overload injury to Ca2+-paradox that would develop during Ca2+-repletion, may be definitely prevented. Sufficiently preserved (Na,K)-ATPase activity, hand in hand with stabilized sarcolemmal structure, may prevent an accumulation of sodium beneath the sarcolemma and consequently also an overexcessive entry of Ca2+ into the myocytes. (Mol Cell Biochem 160/161:257–263, 1996)

Key words

7-oxo-prostacyclin calcium paradox sarcolemmal (Na,K)-ATPase subsarcolemmal sodium subsarcolemmal sodium subsarcolemmal Na/ Ca ratio calcium overload of the heart 

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Attila Ziegelhöffer
    • 1
  • Tatiana Ravingerová
    • 1
  • Andrej Džurba
    • 1
  • Narcisa Tribulová
    • 1
  • Ján Slezák
    • 1
  • Albert Breier
    • 2
  • László Szekeres
    • 3
  1. 1.Institute for Heart ResearchSlovak Academy of SciencesBratislavaSlovak Republic
  2. 2.Institute of Molecular Physiology and GeneticsBratislavaSlovak Republic
  3. 3.Institute of PharmacologyAlbert Szent-Györgyi Medical UniversitySzegedHungary

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