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Alpha-adrenergic reactivity of the microcirculation in conscious spontaneously hypertensive rats

  • Chapter
Biochemistry of Signal Transduction in Myocardium

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 17))

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Abstract

The goal of this study was to determine the functional distribution of α1- and α2-adrenoceptors in the striated muscle microcirculation. Experiments were performed in intact conscious spontaneously hypertensive rats (SHR) that were provided with a dorsal microcirculatory chamber to allow microvascular diameter measurements. Administration of selective α1- and α2-agonists, phenylephrine and azepexole, respectively, induced different patterns of microvascular constriction, α1-Adrenoceptor stimulation showed a preferential constriction of large arteries and venules. The entire arteriolar microvasculature was sensitive to α2-adrenoceptor stimulation, whereas the venular vessels did not respond to azepexole. The selective α1- and α2-antagonists prazosin and yohimbine showed patterns of vasodilator activity comparable to those of the corresponding agonists. The specificity of the drug-induced effects was verified by comparing their effects with those of graded hemorrhage, a non-pharmacological method for blood pressure lowering. In the range of blood pressure decreases comparable to that obtained by α-adrenoceptor antagonists, graded hemorrhage did not influence microvascular diameters. These results show a differential functional distribution of α1- and α2-adrenoceptors along the microvascular tree in striated muscle of conscious SHR.

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References

  1. Bylund DB, Eikenberg DC, Hieble JP, Langer SZ, Leflkowitz RJ, Minneman KP, Molinoff PB, Ruffolo RR, Trendelenburg U: International Union of Pharmacology Nomenclature of Adrenoceptors. Pharmacol Rev 46: 121–134, 1994

    PubMed  CAS  Google Scholar 

  2. Faber JE: In situ analysis of α-adrenoceptors on arteriolar and venular smooth muscle in rat skeletal muscle microcirculation. Circ Res 62: 37–50, 1988

    PubMed  CAS  Google Scholar 

  3. Faber JE, Meininger GA: Selective interaction of α-adrenoceptors with myogenic regulation of microvascular smooth muscle. Am J Physiol 259: H1126–H1133, 1990

    PubMed  CAS  Google Scholar 

  4. McGillivray-Anderson KM, Faber JE: Effects of acidosis on contraction of microvascular smooth muscle by α1- and α2-adrenoceptors. Circ Res 66: 1643–1657, 1990

    PubMed  CAS  Google Scholar 

  5. McGillivray-Anderson KM, Faber JE: Effect of reduced blood flow on α1- and α2-adrenoceptor constriction of rat skeletal muscle microvessels. Circ Res 69: 165–173, 1991

    PubMed  CAS  Google Scholar 

  6. Tateishi J, Faber JE: ATP-sensitive K+ channels mediate α2D-adrenergic receptor contraction of arteriolar smooth muscle and reversal of contraction by hypoxia. Circ Res 76: 53–63, 1995

    PubMed  CAS  Google Scholar 

  7. Faber JE: Effect of local tissue cooling on microvascular smooth muscle and postjunctional α2-adrenoceptors. Am J Physiol 255: H121–H127, 1988

    PubMed  CAS  Google Scholar 

  8. Daniel EE, Shi AG, Wang ZL, Guan YY, Hoo K, Cargoe EJ, Kwan CY: α-Adrenoceptors in vascular smooth muscle: all is not well. Blood Vessels 28: 104–109, 1991

    PubMed  CAS  Google Scholar 

  9. Shi A-G, Ahmad S, Kwan Y, Daniel EE: α-Adrenoceptors in dog mesenteric vessels — subcellular distribution and number of [3H]prazosin and [3H]rauwolscine binding sites. J Cardiovasc Pharmacol 15: 515–525, 1990

    Article  PubMed  CAS  Google Scholar 

  10. Ohyanagi M, Faber JE, Nishigaki K: Differential activation of α1 and α2-adrenoceptors on microvascular smoothmuscle during sympathetic nerve stimulation. Circ Res 68: 232–244, 1991

    PubMed  CAS  Google Scholar 

  11. Smith TL, Osborne SW, Hutchins PM: Long-term micro- and macrocirculatory measurements in conscious rats. Microvasc Res 29: 360–370, 1985

    Article  PubMed  CAS  Google Scholar 

  12. Le Noble JLML, Smith TL, Hutchins PM, Struijker Boudier HAJ: Microvascular alterations in adult conscious spontaneously hypertensive rats. Hypertension 15: 415–419, 1990

    PubMed  Google Scholar 

  13. Messing MWJ, Van Essen H, Smith TL, Smits JFM, Struijker Boudier HAJ: Microvascular actions of calcium channel antagonists. Eur J Pharmacol 198: 189–195, 1991

    Article  PubMed  CAS  Google Scholar 

  14. Lombard JH, Roman RJ: Assessment of muscle blood flow by laser Doppler flowmetry during hemorrhage in SHR. Am J Physiol 259: H860–H865, 1990

    PubMed  CAS  Google Scholar 

  15. Chilian WM: Functional distribution of α1- and α2-adrenergic receptors in the coronary microcirculation. Circulation 84: 2108–2122, 1991

    PubMed  CAS  Google Scholar 

  16. Nielsen H, Thorn SMG, Hughes AD, Martin GN, Mulvany MJ, Sever PS: Postjunctional α1-adrenoceptors mediate vasoconstriction in human subcutaneous resistance vessels. Br J Pharmacol 97: 829–834, 1989

    PubMed  CAS  Google Scholar 

  17. Nielsen H, Mortenson FV, Pilegaard HK, Hasekam JM, Mulvany MJ: Calcium utilization coupled to stimulation of postjunctional α1- and α2-adrenoceptors in isolated human resistance arteries. J Pharmacol Exp Ther 260: 637–643, 1992

    PubMed  CAS  Google Scholar 

  18. Nielsen H, Mortensen FV, Mulvany MJ: Differential distribution of postjunctional α2-adrenoceptors in human omental small arteries. J Cardiovasc Pharmacol 16: 34–40, 1990

    Article  PubMed  CAS  Google Scholar 

  19. Wyse DG: α-Adrenoceptor responses of an isolated human artery and blood pressure. J Vase Res 32: 66–78, 1995

    Article  CAS  Google Scholar 

  20. Nielsen H, Hasenkam JM, Pilegaard HK, Mortensen FV, Mulvany MJ: α-Adrenoceptors in human resistance arteries from colon, pericardial fat, and skeletal muscle. Am J Physiol 261: H762–H767, 1991

    PubMed  CAS  Google Scholar 

  21. Willette RN, Hieble JP, Sauermelch CF: The role of α-adrenoceptor subtypes in sympathetic control of the acral cutaneous microcirculation. J Pharmacol Exp Ther 256: 599–605, 1991

    PubMed  CAS  Google Scholar 

  22. De Mey J, Vanhoutte PM: Uneven distribution of postjunctional α1-and α2-like adrenoceptors in canine arterial and venous smooth muscle. Circ Res 48: 875–884, 1981

    PubMed  Google Scholar 

  23. D’Oyley HM, Pang CCY: Effects of α1- and α2-adrenoceptor antagonists on venous tone in conscious rats. Eur J Pharmacol 182: 283–290, 1990

    Article  PubMed  Google Scholar 

  24. Marshall JM: The influence of the sympathetic nervous system on individual vessels of the microcirculation of skeletal muscle of the rat. J Physiol 332: 169–186, 1982

    PubMed  CAS  Google Scholar 

  25. Struijker Boudier HAJ, Le Noble JLML, Messing MWJ, Huijberts MSP, Le Noble FAC, Van Essen H: The microcirculation and hypertension. J Hypertens 10 (suppl 7): 147–156, 1992

    Article  Google Scholar 

  26. Torres Filho IP, Boegehold MA. Bouskela E, House SD, Johnson PC: Microcirculatory responses in cat sartorius muscle to hemorrhagic hypotension. Am J Physiol 257: H1647–H1655, 1989

    PubMed  CAS  Google Scholar 

  27. Coffman JD: Raynaud’s phenomenon. An update. Hypertension 17: 593–602, 1991

    PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Pharmacology, Cardiovascular Research Institute Maastricht (CARIM), University of Limburg, P.O. Box 616, 6200, MD, Maastricht, The Netherlands

    H. A. J. Struijker-Boudier, M. W. J. Messing & H. van Essen

Authors
  1. H. A. J. Struijker-Boudier
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  2. M. W. J. Messing
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  3. H. van Essen
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Editor information

Editors and Affiliations

  1. Department of Biochemistry, Cardiovascular Research Institute COEUR, Faculty of Medicine of Health Science, Erasmus University Rotterdam, P.O. Box 1738, 3300 DR, Rotterdam, The Netherlands

    Jos M. J. Lamers

  2. Department of Cardiology, Cardiovascular Research Institute COEUR, Erasmus University Rotterdam, P.O. Box 1738, 3300 DR, Rotterdam, The Netherlands

    Pieter D. Verdouw

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© 1996 Kluwer Academic Publishers

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Struijker-Boudier, H.A.J., Messing, M.W.J., van Essen, H. (1996). Alpha-adrenergic reactivity of the microcirculation in conscious spontaneously hypertensive rats. In: Lamers, J.M.J., Verdouw, P.D. (eds) Biochemistry of Signal Transduction in Myocardium. Developments in Molecular and Cellular Biochemistry, vol 17. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1275-8_31

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  • DOI: https://doi.org/10.1007/978-1-4613-1275-8_31

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8544-1

  • Online ISBN: 978-1-4613-1275-8

  • eBook Packages: Springer Book Archive

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