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Comparison of calcium-current in isolated atrial myocytes from failing and nonfailing human hearts

  • Chapter
Biochemistry of Signal Transduction in Myocardium

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 17))

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Abstract

To identify possible alterations of the L-type calcium currents (ICa,L) in cardiomyopathy, ICa,L were recorded in atrial myocytes dissociated from the nonfailing heart (NF) of patients undergoing corrective open-heart surgery and explanted failing heart (FH) of patients with dilated cardiomyopathy undergoing heart transplantation. The patch-clamp technique was applied in the single-electrode whole-cell mode. The electrophysiological properties of ICa,L, including cell capacitance and current density, were similar in atrial myocytes from both groups of patients. Further to identify possible alterations of the myocardial beta-adrenergic pathway in cardiomyopathy, we examined the effects of isoproterenol, forskolin, 8-Br-cAMP and IBMX on ICa,L in both groups of atrial myocytes. Perfusion of isoproterenol (1 µM) significantly increased the peak ICa,L by 515 ± 44% in 6 atrial myocytes from NF but increased only by 135 ± 25% in 27 atrial myocytes from FH. However, forskolin (1 µM) or 8-Br-cAMP (0.1 mM) increased the peak ICa,L to a similar extent in atrial myocytes from NF and FH. IBMX (20 µM) also induced a comparable increase in the peak ICa,L by 213 ± 31% (n=5) and 207 ± 59% (n=4) in atrial myocytes from NF and FH, respectively. The above findings suggest that in atrial myocytes obtained from FH the beta-adrenoceptor numbers might be decreased but no impairment of the signal transduction cascade occurred beyond the GTP binding proteins level.

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Authors and Affiliations

  1. Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, Republic of China

    Tzu-Hurng Cheng

  2. Department of Surgery, National Defense Medical Center, Taipei, Taiwan, Republic of China

    Fan-Yen Lee & Jeng Wei

  3. Department of Pharmacology, National Defense Medical Center, P.O. Box 90048, Taipei, Taiwan, Republic of China

    Tzu-Hurng Cheng & Cheng-I Lin

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  1. Tzu-Hurng Cheng
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  2. Fan-Yen Lee
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  3. Jeng Wei
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  4. Cheng-I Lin
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Editor information

Editors and Affiliations

  1. Department of Biochemistry, Cardiovascular Research Institute COEUR, Faculty of Medicine of Health Science, Erasmus University Rotterdam, P.O. Box 1738, 3300 DR, Rotterdam, The Netherlands

    Jos M. J. Lamers

  2. Department of Cardiology, Cardiovascular Research Institute COEUR, Erasmus University Rotterdam, P.O. Box 1738, 3300 DR, Rotterdam, The Netherlands

    Pieter D. Verdouw

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© 1996 Kluwer Academic Publishers

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Cheng, TH., Lee, FY., Wei, J., Lin, CI. (1996). Comparison of calcium-current in isolated atrial myocytes from failing and nonfailing human hearts. In: Lamers, J.M.J., Verdouw, P.D. (eds) Biochemistry of Signal Transduction in Myocardium. Developments in Molecular and Cellular Biochemistry, vol 17. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1275-8_20

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  • DOI: https://doi.org/10.1007/978-1-4613-1275-8_20

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8544-1

  • Online ISBN: 978-1-4613-1275-8

  • eBook Packages: Springer Book Archive

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