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Tissue reconstitution, or transgenic mammary gland, technique for modeling breast cancer development

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Mammary Tumor Cell Cycle, Differentiation, and Metastasis

Part of the book series: Cancer Treatment and Research ((CTAR,volume 83))

Abstract

Breast cancer appears to develop as a result of clones of cells accumulating a series of mutations. To model this process, we [1–9] and others [10–13] have introduced tumor mutations into individual clones of cells in mouse mammary epithelium, in mice, by a transplantation approach known as tissue reconstitution or constructing a transgenic mammary gland. Introducing oncogenes and growth factor genes in this way has shown how the three-dimensional growth pattern of mammary epithelium is altered by such genes and allows us to address basic questions, such as: What does an individual oncogene do to three-dimensional growth control? Do related oncogenes have similar or different effects? How do the effects of oncogenes relate to their normal role in controlling the three-dimensional growth pattern? How do clones of cells that express an oncogene behave among neighboring normal cells in an epithelium? The method also allows sequential introduction of more than one oncogene to follow tumor development.

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Authors
  1. Paul A. W. Edwards
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Editors and Affiliations

  1. Departments of Cell Biology and Pharmacology, Lombardi Cancer Center, The Research Building, 3970 Reservoir Road, 20007, Washington, DC, USA

    Robert B. Dickson PHD

  2. Departments of Medicine and Pharmacology, Lombardi Cancer Center, The Research Building, 20007, 3970 Reservoir Road, Washington, CD, USA

    Marc E. Lippman MD

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© 1996 Kluwer Academic Publishers

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Edwards, P.A.W. (1996). Tissue reconstitution, or transgenic mammary gland, technique for modeling breast cancer development. In: Dickson, R.B., Lippman, M.E. (eds) Mammary Tumor Cell Cycle, Differentiation, and Metastasis. Cancer Treatment and Research, vol 83. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1259-8_2

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  • DOI: https://doi.org/10.1007/978-1-4613-1259-8_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8536-6

  • Online ISBN: 978-1-4613-1259-8

  • eBook Packages: Springer Book Archive

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