Mitogenic and Non-Mitogenic Induction of Lymphocytic Invasion: Dual Parameter flow Cytometric Analysis
It has been well established that mitogenic activation produces profound alterations in the migratory behavior of lymphocytes. Activated, blastic T and B cells down-regulate homing receptors which mediate adhesion to the high endothelium of peripheral lymph nodes (1–3). Concurrently, they up-regulate adhesion receptors for extracellular matrix components and for ligands typical of inflammatory endothelium (4–6). The result is a change in traffic patterns, from extravasation into lymph nodes to extravasation into nonlymphoid tissue, especially inflamed tissue (7,8). After the immune response terminates, memory cells, the long-lived residual progeny of the blasts, remain. Memory helper T cells, even when not proliferating, retain the non-homing, inflammation-seeking behavior of the parental blasts (9–11). There are indications that other memory lymphocyte subsets follow similar trends (12,13).
KeywordsAcridine Orange Peripheral Lymph Node Cell Cycle Profile Homing Receptor Lymph Node Homing
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