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In Situ Hybridization of Centromeric DNA as Visualized on the Nuclear Matrix by Laser Confocal Cytometry

  • Kenneth J. Pienta
Part of the Developments in Oncology book series (DION, volume 77)

Abstract

An average mammalian nucleus contains approximately 2 meters of DNA in its extended first order structure. The packing of this length of DNA into a nucleus of only 10 µm diameter presents mammalian cells with a formidable topological packaging problem since the total length of cellular DNA must be reduced about 10,000-fold to fit within the confines of a single nucleus (1). Despite this tremendous DNA packing ratio, DNA contained within nuclei must have a dynamic conformation conducive to an active role in a variety of biologic processes. For example, the replication of the DNA occurs in 30,000 to 90,000 small units termed “replicons” that are synthesized in a precise order and temporal sequence. During DNA synthesis, each of these DNA replicon units must be copied by passing through a very large multienzyme replicating complex (5x10 d) that contains the integrated biochemical site for DNA synthesis; these enzyme complexes have been termed “replisomes” (2,3).

Keywords

Nuclear Matrix Nuclear Matrix Protein Cell BioI Chicken Oviduct Steroid Hormone Receptor Binding 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Kenneth J. Pienta

There are no affiliations available

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