In Situ Hybridization of Centromeric DNA as Visualized on the Nuclear Matrix by Laser Confocal Cytometry
An average mammalian nucleus contains approximately 2 meters of DNA in its extended first order structure. The packing of this length of DNA into a nucleus of only 10 µm diameter presents mammalian cells with a formidable topological packaging problem since the total length of cellular DNA must be reduced about 10,000-fold to fit within the confines of a single nucleus (1). Despite this tremendous DNA packing ratio, DNA contained within nuclei must have a dynamic conformation conducive to an active role in a variety of biologic processes. For example, the replication of the DNA occurs in 30,000 to 90,000 small units termed “replicons” that are synthesized in a precise order and temporal sequence. During DNA synthesis, each of these DNA replicon units must be copied by passing through a very large multienzyme replicating complex (5x10 d) that contains the integrated biochemical site for DNA synthesis; these enzyme complexes have been termed “replisomes” (2,3).
KeywordsFormaldehyde Cage Adenocarcinoma Fractionation Iodide
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