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Unique and selective mitogenic effects of vanadate on SV40-transformed cells

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Vanadium Compounds: Biochemical and Therapeutic Applications

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 16))

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Abstract

Vanadate and insulin both function as unique complete mitogens for SV40-transformed 3T3T cells, designated CSV3-1, but not for nontransformed 3T3T cells. The mitogenic effects induced by vanadate and insulin in CSV3-1 cells are mediated by different signaling mechanisms. For example, vanadate does not stimulate the tyrosine phosphorylation of the insulin receptor β-subunit nor the 170 kDa insulin receptor substrate-1. Instead, vanadate induces a marked increase in tyrosine phosphorylation of 55 and 64 kDa proteins that is not observed in insulin-stimulated CSV3-1 cells. Perhaps most interestingly, vanadate-in-duced mitogenesis is associated with the selective induction of c-jun and junB expression without significantly inducing c-fos or c-myc. Furthermore, treatment of CSV3-1 cells with genistein abolishes the effects of vanadate on protein tyrosine phosphorylation and c-jun induction. These and related data suggest that modulation of protein tyrosine phosphorylation and c-jun and junB expression may serve the critical roles in mediating vanadate-induced mitogenesis in SV40-transformed cells.

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  1. Department of Pathology, The University of Tennessee College of Medicine, 899 Madison Avenue, Memphis, Tennessee, 38163, USA

    Hanlin Wang & Robert E. Scott

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  1. Centre de Recherche, Hotel-Dieu de Montreal, 3850, rue Saint Urbain, Pavillion Marie de la Ferre, Montreal, Quebec, H2W 1T8, Canada

    Ashok K. Srivastava  & Jean-Louis Chiasson  & 

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Wang, H., Scott, R.E. (1995). Unique and selective mitogenic effects of vanadate on SV40-transformed cells. In: Srivastava, A.K., Chiasson, JL. (eds) Vanadium Compounds: Biochemical and Therapeutic Applications. Developments in Molecular and Cellular Biochemistry, vol 16. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1251-2_7

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  • DOI: https://doi.org/10.1007/978-1-4613-1251-2_7

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