Regulation and control of glucose overutilization in erythrocytes by vanadate

  • Najma Zaheer Baquer
  • Amit Kumar Saxena
  • Poonam Srivastava
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 16)


The insulin mimetic effect of vanadate in in vitro incubation of erythrocytes with high glucose concentrations showed an increase in sorbitol accumulation and glucose utilization using U-14C-glucose. Aldose reductase inhibitors and vanadate addition reversed the sorbitol accumulation, whereas insulin could not reverse it. Increased glucose utilization was also normalized with vanadium compounds. Increased activity of aldose reductase and sorbitol levels in diabetic animals were also normalized with vanadate treatment.

Key words

insulin mimetic vanadate RBC glucose utilization aldose reductase 


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  1. 1.
    Travis SF, Morrison AD, Clements RS, Winegred AL, Oski FA: Metabolic alterations in human erythrocyte sorbitol produced by increase in glucose concentration. J Clin Invest 50: 2104–2111, 1971PubMedCrossRefGoogle Scholar
  2. 2.
    Das B, Srivastava SK: Purification and properties of aldose reductase and aldehyde reductase II from human erythrocyte. Arch Biochem Biophys 238: 670–679, 1985PubMedCrossRefGoogle Scholar
  3. 3.
    Hamada Y, Kito R, Raskin P: Increased erythrocyte aldose reductase activity in Type I diabetic patients. Diabetic Med 226–231, 1991Google Scholar
  4. 4.
    Srivastava SK, Ansari NH, Hair G A, Jaspan J, Rao MB, Das B: Hyperglyeaemia induced activation of human erythrocyte aldose reductase and alteration in kinetic properties. Biochem Biophys Acta 870: 302–331, 1986PubMedCrossRefGoogle Scholar
  5. 5.
    Robey C, Mahapatra AD, Cohen MP, Suarez S: Sorbinil partially prevents decreased erythrocyte deformibility in experimental diabetes mellitus. Diabetes 36: 1010–1013, 1987PubMedCrossRefGoogle Scholar
  6. 6.
    Saxena AR, Srivastava P, Kale RK, Baquer NZ: Effect of vanadate administration on polyol pathway in diabetic rat kidney. Biochem Int 26: 59–68, 1992PubMedGoogle Scholar
  7. 7.
    Brownlee M, Cerami A: The Biochemistry of the Complications of diabetes. Ann Rev Biochem 50: 385 432, 1981Google Scholar
  8. 8.
    Gabay RH: The sorbitol pathway and complications of diabetes. N Engl J Med 288: 831–836, 1973CrossRefGoogle Scholar
  9. 9.
    Sochor M, Baquer NZ, McLean P: Glucose over and underutilization in diabetes: Comparative studies on the changes in activities of enzymes of glucose metabolism in rat kidney and liver. Mol Physiol 7: 51–68, 1985Google Scholar
  10. 10.
    Malone JI, Knox G, Benford S, Tedasco TA: Red cell sorbitol: An indicator of diabetic control. Diabetes 29: 861–864, 1980PubMedCrossRefGoogle Scholar
  11. 11.
    Hothersall JS, Baquer NZ, McLean P: Pathways of carbohydrate metabolism in peripheral nervous tissue. The contribution of alternative route of glucose utilization in peripheral nerve and brain. Enzyme 27: 259–267, 1982PubMedGoogle Scholar
  12. 12.
    Van Kampen EJ, Zijlstra WG: Standardization of haemalobinometery H. The Hemiglobinicyanide method. Clin Chem Acta 6: 538–544, 1961CrossRefGoogle Scholar
  13. 13.
    Saxena AK, Srivastava P, Baquer NZ: Effect of vanadate on glycolytic enzymes and malic enzyme in insulin dependent and independent tissues of diabetic rats. Eur J Pharmacol 216: 123–126, 1992PubMedCrossRefGoogle Scholar
  14. 14.
    Saxena AK, Srivastava P, Kale RK, Baquer NZ: Impaired antioxidant status in diabetic rat liver: Effect of vanadate. Biochem Pharmacol 40: 539–542, 1993CrossRefGoogle Scholar
  15. 15.
    Srivastava P, Saxena AK, Kale RK, Baquer NZ: Insulin like effects of lithium and vanadate on the altered antioxidant status of diabetic rats. Res Commun Chem Pathol Pharmacol 80(3): 283–293, 1993PubMedGoogle Scholar
  16. 16.
    Heyliger CE, Tahiliani AG, McNeil JH: Effect of vanadate on elevated blood glucose and depressed cardiac performance of diabetic rats. Science 227: 1474–1476, 1985PubMedCrossRefGoogle Scholar
  17. 17.
    Pugazenthi S, Khandelwal RE: Insulin like effects of vanadate on hepatic glycogen metabolism in non-diabetic and streptozotocin-induced diabetic rats. Diabetes 39: 821–827, 1990CrossRefGoogle Scholar
  18. 18.
    Brichard SM, Desguois B, Girard J: Vanadate of diabetic rats reverses the impaired expression of genes involved in hepatic glucose metabolism: Effect of glycolytic and gluconeogenic enzyme and on glucose transporter GLUT-2. Mol Cell Endocrinol 8: 81–97, 1993Google Scholar
  19. 19.
    Valera A, Rodriguez-Gil JE, Bosoh F: Vanadate treatment restores the expression of genes for the key enzymes in the glucose and ketone bodies metabolism in the liver of diabetic rats. J Clin Invest 29: 4–11, 1993CrossRefGoogle Scholar
  20. 20.
    Guan A: The insulin like effect of sodium vanadate on adipocyte glucose transport mediated port insulin receptor level. Biochem J 238: 665–669, 1986Google Scholar
  21. 21.
    Blondel O, Sison J, Chevalier B, Bernard P: Impaired insulin action but normal insulin receptor activity in diabetic rat liver: Effect of vanadate. Endocrinol Med 258: E459–E467; 1990Google Scholar
  22. 22.
    Strout HV, Vicario PP, Biswas C, Saperstein R, Brady EJ, Plich PF, Berger J: Vanadate treatment of streptozotocin diabetic rats restores expression of the insulin responsive glucose transporter in skeletal muscle. Endocrinol 126: 2728–2732, 1990CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Najma Zaheer Baquer
    • 1
  • Amit Kumar Saxena
    • 1
  • Poonam Srivastava
    • 1
  1. 1.School of Life SciencesJawaharlal Nehru UniversityNew DelhiIndia

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