Changes in Contractile Proteins under Oxidative Stress
Oxygen free radicals have been demonstrated to have the capacity to cause cardiac contractile dysfunction [1–4]. It is well known that cardiac contraction is produced by the interaction between actin and myosin and that the energy for the contraction is supplied through the myosin ATPase reaction . Myofibrillar creatine kinase has been demonstrated to be functionally coupled to myosin ATPase [6–8] and serves as an important intramyofibrillar ATP-regenerating system (phosphocreatine energy shuttle) . Therefore, alterations in myofibrillar creatine kinase activity may disturb energy utilization by the myocardium and may result in contractile dysfunction. In fact, it has been reported that inhibition of creatine kinase activity can result in dysfunction of the heart, especially at increased work loads .
KeywordsCreatine Kinase Xanthine Oxidase Oxygen Free Radical Sulfhydryl Group Creatine Kinase Activity
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- 4.Przyklenk K, Whittaker P, Kloner RA. 1988. Direct evidence that oxygen free radicals cause contractile dysfunction in vivo. Circulation 78 (Suppl II):II–264.Google Scholar
- 5.Saks VA, Ventura-Clapier R, Huchua ZA, Preobrazhensky AN, Emelin IV. 1984. Creatine kinase in regulation of heart function and metabolism. I. Further evidence for compart-mentation of adenine nucleotides in cardiac myofibrillar and sarcolemmal coupled ATPase-creatine kinase systems. Biochem Biophys Acta 803:254–264.PubMedCrossRefGoogle Scholar
- 7.Bessman SP, Yang WCT, Geiger PJ, Erickson-Viitanen S. Intimate coupling of creatine Phosphokinase and myofibrillar adenosine triphosphatase. Biochem Biophys Res Commun 96:1414–1420.Google Scholar
- 8.Yagi K, Mase R. 1984. Coupled reaction of creatine kinase and myosin A-adenosine triphosphatase. J Biol Chem 259:5238–5246.Google Scholar
- 10.Kaneko M, Beamish RE, Dhalla NS. 1989. Depression of heart sarcolemmal Ca2+-pump activity by oxygen free radicals. Am J Physiol 256 (Heart Circ Physiol 25):H368–H374.Google Scholar
- 20.Yamada S, Ikemoto N. 1987. Distinction of thiols involved in the specific reaction steps of the Ca2+-ATPase of the sarcoplasmic reticulum. J Biol Chem 253:6801–6807.Google Scholar
- 26.Bittle JA, Weisfeldt ML, Jacobus WE. 1985. Creatine kinase of heart mitochondria: progressive loss of energy activity during in vivo ischemia and its correlation to depressed myocardial function. J Biol Chem 260:208–214.Google Scholar