Molecular Cloning of the Maize R-nj Allele by Transposon Tagging with Ac

  • Stephen L. Dellaporta
  • Irwin Greenblatt
  • Jerry L. Kermicle
  • James B. Hicks
  • Susan R. Wessler
Part of the Stadler Genetics Symposia Series book series (SGSS)

Abstract

The R locus of maize is one of several genes that condition the red and purple anthocyanin pigments throughout the body of the plant and seed. Many alleles of the R locus have been described, each determining a different pattern of pigment distribution in the plant. Genetic studies have lead to the proposal that R is a complex locus containing several similar but functionally distinct modules that regulate anthocyanin expression in specific organs or tissues. We report on the isolation of a segment of DNA from the R locus using the controlling element Activator (Ac)as a transposon tag. Molecular evidence is presented confirming the complex organization of this regulatory locus. The identification of cross-hybridizing restriction fragments corresponding to the tissue-specific R components suggests that these elements, while functionally divergent in tissue-specificity, have retained significant DNA homology and may have evolved from a common ancesteral component at R.

Keywords

Maize Chlorophyll EDTA Recombination Agarose 

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Stephen L. Dellaporta
    • 1
  • Irwin Greenblatt
    • 2
  • Jerry L. Kermicle
    • 3
  • James B. Hicks
    • 4
  • Susan R. Wessler
    • 5
  1. 1.Department of BiologyYale UniversityNew HavenUSA
  2. 2.Molecular and Cellular BiologyUniversity of ConnecticutStorrsUSA
  3. 3.Laboratory of GeneticsUniversity of WisconsinMadisonUSA
  4. 4.Department of Molecular BiologyScripps Clinic and Research FoundationLa JollaUSA
  5. 5.Department of BotanyUniversity of GeorgiaAthensUSA

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