Molecular Cloning of the Maize R-nj Allele by Transposon Tagging with Ac

  • Stephen L. Dellaporta
  • Irwin Greenblatt
  • Jerry L. Kermicle
  • James B. Hicks
  • Susan R. Wessler
Part of the Stadler Genetics Symposia Series book series (SGSS)


The R locus of maize is one of several genes that condition the red and purple anthocyanin pigments throughout the body of the plant and seed. Many alleles of the R locus have been described, each determining a different pattern of pigment distribution in the plant. Genetic studies have lead to the proposal that R is a complex locus containing several similar but functionally distinct modules that regulate anthocyanin expression in specific organs or tissues. We report on the isolation of a segment of DNA from the R locus using the controlling element Activator (Ac)as a transposon tag. Molecular evidence is presented confirming the complex organization of this regulatory locus. The identification of cross-hybridizing restriction fragments corresponding to the tissue-specific R components suggests that these elements, while functionally divergent in tissue-specificity, have retained significant DNA homology and may have evolved from a common ancesteral component at R.


Flank Marker Anthocyanin Pigmentation Reciprocal Chromosomal Translocation Standard Allele Aleurone Color 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Behrens, U., N. Fedoroff, A. Laird, M. Muller-Neumann, P. Starlinger, and J. Yoder, 1984, Cloning of the Zea mays controlling element Ac from the wx-m7 allele, Mol. Gen. Genet. 194: 346–347.CrossRefGoogle Scholar
  2. Berman, M.L., L.W. Enquist,, and T.J. Silhavy, 1982, Advanced Bacterial Genetics, Cold Spring Harbor, N.Y. City.Google Scholar
  3. Brink, R.A. and E. Williams, 1973, Mutable R-navajo alleles of cyclic origin in maize, Genetics 73: 273–296.PubMedGoogle Scholar
  4. Chomet, P.S., S. Wessler, and S.L. Dellaporta, 1986, Inactivation of the maize transposable element Activator is associated with its DNA modification. EMBO J.Google Scholar
  5. Dooner, H.K., 1983, Coordinate genetic regulation of flavonoid biosynthetic enzymes in maize, Mol Gen. Genet. 189: 136–141.CrossRefGoogle Scholar
  6. Dononer, H.K., and J.L. Kermicle, 1976, Displaced and tandem duplications in the long arm of chromosome 10 in maize, Genetics 82: 309–322.Google Scholar
  7. Donner, H.K., and J.L. Kermicle, 1974, Reconstitution of the R-r compound allele in maize, Genetics 78: 691–701.Google Scholar
  8. Dooner, H.K., and J.L. Kermicle, 1971, Structure of the R-r tandem duplication in maize, Genetics 67: 427–436.PubMedGoogle Scholar
  9. Dretzen, G., M. Bellard, P. Sassone-Corsi, and P. Chambon, 1981, A reliable method for the recovery of DNA fragments from agarose and acrylamide gels, Anal Biochem. 112: 295.PubMedCrossRefGoogle Scholar
  10. Fedoroff, N.V., D.B. Furtek,, and O.E. Nelson, Jr., 1984, Cloning of the bronze locus in maize by a simple and generalizable procedure using the transposable controlling element Ac, Proc. Natl. Acad. Sci.. U.S.A. 81: 3825–3829.PubMedCrossRefGoogle Scholar
  11. Fedoroff, N., S. Wessler, and M. Shure, 1983, Isolation of the transposable maize controlling elements Ac and Ds, Cell 35: 235–242.PubMedCrossRefGoogle Scholar
  12. Greenblatt, I.M., 1984, A chromosomal replication pattern deduced from pericarp phenotypes resulting from movements of the transposable element, Modulator, in maize, Genetics 108: 471–485.PubMedGoogle Scholar
  13. Greenblatt, I.M., and R.A.Brink, 1963 Transpositions of Modulator in maize into divided and undivided chromosome segments, Nature 197: 412–413.CrossRefGoogle Scholar
  14. Greenblatt, I.M., and R.A. Brink, 1962, Twin mutations in medium variegated pericarp maize, Genetics 47: 489–501.PubMedGoogle Scholar
  15. Hohn, B., 1979 In vitro packaging of X and cosmid DNA, Methods Enzvmol. 68: 299.CrossRefGoogle Scholar
  16. Holmes, D.S., and M. Quigley, 1981, A rapid boiling method for the preparation of bacterial plasmids. Anal. Biochem. 119: 193.CrossRefGoogle Scholar
  17. Kermicle, J.L., 1985, Alternative tests of allelism. in: “Plant Genetic,” UCLA Symp. on Molecular and Cellular Biology, vol. 35, M. Freeling ed, Alan R. Liss, Inc., City, pp. 491–507.Google Scholar
  18. Kermicle, J.L., 1980, Probing the component structure of a mize gene with transposable elements, Science 108: 1457–1459.CrossRefGoogle Scholar
  19. Kermicle, J.L., 1970, Somatic and meiotic instability of R-strippled, an aleurone spotting factor in maize, Genetics 64: 247–258.PubMedGoogle Scholar
  20. Kermicle, J.L., and J.D. Axtell, 1981, Modification of chlorophyll striping by the R region, Maydica 26: 185–197.Google Scholar
  21. Lechelt, C., A. Laird, and P. Starlinger, 1986, Cloning DNA from the P locus, Maize Genet. Coop. Newsletter 60: 40.Google Scholar
  22. Maniatis, T., E.F. Fritsch,, and J. Sambrook, 1982, “Molecular Cloning: A Laboratory Manual,” Cold Spring Harbor Laboratory, City.Google Scholar
  23. Martin, B., A. Gierl, P. Peterson, and H. Saedler, 1986 Molecular cloning of the A2 locus, Maize Genet. Coop. Newsletter 60: 41–42.Google Scholar
  24. McClelland, M., 1982 The effect of sequence specific DNA methylation on restriction endonuclease cleavage, Nucleic Acid Res. 9: 5859–5866.CrossRefGoogle Scholar
  25. McClintock, B., 1954, Mutations in maize and chromosomal aberrations in Neurospora, Carnegie Inst. Washington Year Book 53: 254–260.Google Scholar
  26. McClintock, B., 1948, Mutable loci in maize, Carnegie Inst. Washington Year Book 47: 155–169.Google Scholar
  27. McWhirter, K.S., and R.A. Brink,. 1962, Continuous variation in level of parmutation at the R locus in maize, Genetics 47: 1053–1074.PubMedGoogle Scholar
  28. Muller-Neumann, M., J. Yonder, and P. Starlinger, 1985, The sequence of the Ac element of Zea mays, Mol, Gen. Genet. (In press).Google Scholar
  29. O’Reilly, C.O., N.S. Shepard, A. Pereira, Zs. Schwarz-Sommer, I. Bertram, D.S. Robertson, P. A. Peterson, and H. Saedler, 1985, Molecular cloning of the al locus of Zea mays using the transposable elements En and Spm, EMBO J. 4: 887–882.Google Scholar
  30. Paz-Ares, J., U. Wienand, P.A. Peterson, and H. Saedler, 1986, Molecular cloning of the c locus of Zea mays, EMBO J. 5: 829–834.PubMedGoogle Scholar
  31. Pohlman, R., N.V. Fedoroff, and J. Messing, 1984, The nucleotide sequence of the maize controlling element Activator, Cell 37: 635–643.PubMedCrossRefGoogle Scholar
  32. Rigby, P.W.J., M. Dieckmann, C. Rhodes, and P. Berg, 1977, Labeling deozxyridonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I., J. Mol. Biol. 113, 237–251.PubMedCrossRefGoogle Scholar
  33. Shure, M., S. Wessler, and N. Fedoroff, 1983, Molecular identification and isolation of the Waxy locus in maize, Cell 35: 225–233.PubMedCrossRefGoogle Scholar
  34. Southern, E., 1975, Detection of specific sequences among DNA fragments separated by gel electrophoresis, J. Mol. Biol. 98: 503.PubMedCrossRefGoogle Scholar
  35. Stadler, L.J., 1951, Spontaneous mutation in maize. Cold Spring Harbor Svmp. Quant. Biol. 16: 49–63.Google Scholar
  36. Styles, E.D., O. Ceska, and -K-T.Seah, 1973, Developmental differences in action ofi? and B alleles in maize, Can. J. Genet. Cytol. 15: 59–72.Google Scholar
  37. Theres, K., and P. Starlinger, 1986, Molecular cloning of bz2-m, Maize Genet. Coop. Newsletter 60: 40.Google Scholar
  38. Van Schaik, N.W., and R.A. Brink, 1959, Transpositions of Modulator, a component of the variegated pericarp allele in maize, Genetics 44: 725–738.PubMedGoogle Scholar
  39. Wienand, U., U.Weydemann, U. Niesbach-Klosgen, P. Peterson, and H. Saedler, 1986 Molecular analysis of the c2 locus, Maize Genet. Coop. Newsletter 60: 42.Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Stephen L. Dellaporta
    • 1
  • Irwin Greenblatt
    • 2
  • Jerry L. Kermicle
    • 3
  • James B. Hicks
    • 4
  • Susan R. Wessler
    • 5
  1. 1.Department of BiologyYale UniversityNew HavenUSA
  2. 2.Molecular and Cellular BiologyUniversity of ConnecticutStorrsUSA
  3. 3.Laboratory of GeneticsUniversity of WisconsinMadisonUSA
  4. 4.Department of Molecular BiologyScripps Clinic and Research FoundationLa JollaUSA
  5. 5.Department of BotanyUniversity of GeorgiaAthensUSA

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