Use of New Fluorescent Triacylglycerols for Determining Lipase Activities and Diagnosing Wolman Disease

  • Nicole Dousset
  • Anne Nègre
  • Valerie Réaud
  • Quoc Quan Dang
  • Pierre Rogalle
  • Robert Salvayre
  • Louis Douste-Blazy
Conference paper
Part of the NATO ASI Series book series (NSSA, volume 150)

Abstract

Two kinds of lipase, lipoprotein lipase (EC 3.1.1.34) and hepatic triacylglycerol lipase (EC 3.1.1.3) are released into plasma when heparin is intravenously administered. Lipoprotein lipase catalyzes the hydrolysis of triacylglycerols bound in chylomicrons and very-low-density lipoproteins and hepatic triacylglycerol lipase the hydrolysis of triacylglycerols of intermediate density (1). These enzymes exhibited activity using natural or fluorescent synthetic triacylglycerol (2).

Keywords

Hydrolysis Glycerol Lipase Heparin Carboxyl 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    A. Rubinstein, J.C. Gibson, J.R. Paterniti, G. Kakis, A. Little, H.N. Ginsberg, W.V. Brown (1985). Effect of heparin-induced lipolysis on the distribution of apolipoprotein E among lipoprotein subclasses. Studies with patients deficient in hepatic triglyceride lipase and lipoprotein lipase. J. Clin. Invest., 75, 710–721.PubMedCrossRefGoogle Scholar
  2. 2.
    N. Dousset, A. Negre, R. Salvayre, P. Rogalle, Q.Q. Dang, L. Douste-Blazy. Use of fluorescent radiolabelled triacylglycerol as a substrate for lipoprotein lipase and hepatic triglyceride lipase. Lipids (submitted for publication).Google Scholar
  3. 3.
    A. Negre, R. Salvayre, P. Durand, G. Lenoir, L, Douste-Blazy, (1984), Enzyme studies on Epstein-Barr virus-transformed lymphoid cell lines from Wolman*s disease. Lipases, cholesterol esterase and 4-methylumbelliferyl acyl ester hydrolases. Biochim. Biophys. Acta, 794, 89–95.PubMedGoogle Scholar
  4. 4.
    A. Negre, R, Salvayre, A, Dagan, S, Gatt (1985), New fluorometric assay of lysosomal acid lipase and its application to the diagnosis of Wolman and cholesteryl ester storage diseases, Clin. Chim. Acta, 149, 81–88.PubMedCrossRefGoogle Scholar
  5. 5.
    B. Neises, W. Steglich (1978). Simple method for the esterification of carboxylic acids. Angew. Chem, Int, Ed, Engl., 17, 522–523.CrossRefGoogle Scholar
  6. 6.
    R.G. Jensen, R,E, Pitas, (1976), Synthesis of some acylglycerols and phosphoglycerides. In: “Adv. Lipid Res.”, 14, pp. 213–247, Academic Press, New York, ed. R. Paoletti, D. KritchevskyGoogle Scholar
  7. 7.
    P. Nilsson-Ehle, R. Ekman (1977), Rapid, simple and specific assays for lipoprotein lipase and hepatic lipase. Artery, 3, 194-209Google Scholar
  8. 8.
    P. Belfrage, M. Vaughan (1969), Simple liquid-liquid partition system for isolation of labeled oleic acid from mixtures with glycerides. J. Lipid. Res., 10, 341-343.Google Scholar
  9. 9.
    M.L. Baginsky, W.V. Brown (1979). A new method for the measurement of lipoprotein lipase in post heparin plasma using sodium dodecylsulfate for the inactivation of hepatic triglyceride lipase, J, Lipid, Res. 20, 548-556.Google Scholar
  10. 10.
    N.H. Morley, A, Kuksis, D, Buchnea, J,J, Myher (1975), Hydrolysis of diacylglycerols by lipoprotein lipase. J. Biol. Chem,, 250, 3414-3418Google Scholar
  11. 11.
    N. Morley, A. Kuksis (1977). Lack of fatty acid specificity in the lipolysis of oligo-and polyunsaturated triacylglycerols by milk lipoprotein lipase. Biochim. Biophys. Acta, 487, 332-342.Google Scholar
  12. 12.
    C.S. Wang, A. Kuksis, F. Manganaro (1982). Studies on the substrate specificity of purified human milk lipoprotein lipase. Lipids, 17, 278-284.Google Scholar
  13. 13.
    C, Ehnolm, W, Shaw, H, Greten, W.V. Brown (1975), Purification from human plasma of a heparin-released lipase with activity against triglyceride and phospholipids. J, Biol, Chem., 250, 6756–6761.Google Scholar
  14. 14.
    P.K.J, Kinnunen, C. Ehnolm (1976), Effect of serum and C-apoproteins from very low density lipoprotein on human post heparin plasma hepatic lipase. FEBS Lett., 65, 354-357.Google Scholar
  15. 15.
    M.L. Baginsky (1981). Measurement of lipoprotein lipase and hepatic triglyceride lipase in human post heparin plasma. In: “Methods in enzymology” 72, pp, 325–338, Academic Press, New York, ed, J.M. Lowenstein.Google Scholar
  16. 16.
    A. Negre, R. Salvayre, P. Rogalle, Q.Q. Dang, L, Douste-Blazy,(1987) Acyl-chain specificity and properties of cholesterol esterases from normal and Wolman lymphoid cell lines. Biochim. Biophys, Acta, 918, 76–82.Google Scholar
  17. 17.
    B.D. Lake, A.D. Patrick (1970). WolmanTs disease: deficiency of E600-resistant acid esterase activity with storage of lipids in lysosomes. J. Pediatr., 76, 262–266.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Nicole Dousset
    • 1
  • Anne Nègre
    • 1
  • Valerie Réaud
    • 1
  • Quoc Quan Dang
    • 1
  • Pierre Rogalle
    • 1
  • Robert Salvayre
    • 1
  • Louis Douste-Blazy
    • 1
  1. 1.Laboratoire de Biochimie et INSERM 101, C.H.U. PurpanToulouse CedexFrance

Personalised recommendations