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Development and Regulation of Acetylcholine Receptor Function

  • Chapter
Calcium and Ion Channel Modulation

Abstract

Nicotinic acetylcholine (ACh) receptors are among the first proteins to be expressed on the membranes of newly differentiated muscle cells. During development, the functional properties of the receptors and their associated channels undergo major changes in many skeletal muscles (Schuetze and Role, 1987). The kind of changes as well as the timetable of the changes depends on the type of muscle being studied (Schuetze, 1980; Kullberg and Owens, 1986). Changes in receptor expression also occur in adult skeletal muscle following denervation (Neher and Sakmann, 1976) and during subsequent reinnervation (Brenner and Sakmann, 1983). Studies on adult muscle have led to the proposition that the development of receptor function depends on the proximity of the receptor to the site of nerve-muscle contact (Cull-Candy et al., 1982; Brenner and Sakmann, 1983). The mechanisms which underlie the regulation of receptor function in developing and adult muscle have been the subject of a large number of studies. In this paper we summarize the results of our studies on the development of ACh receptor function in amphibian muscle and include, as well, the results of complementary studies on both innervated and denervated adult mammalian muscle. We address the issues related to neural regulation of ACh receptor properties by comparing properties of synaptic and nonsynaptic receptors in developing and adult muscle. The possible underlying molecular mechanisms and functional significance of our observations are discussed.

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References

  • Anderson, M.J., Cohen, M.W., Zorychta, E., 1977, Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells, J. Physiol., 268:731.

    Google Scholar 

  • Axelsson, J. and Thesleff, F., 1959, A study of supersensitivity in denervated mammalian skeletal muscle, J. Physiol., 149:178.

    Google Scholar 

  • Bevan, S. and Steinbach, J.H., 1977, The distribution of alpha-bungarotoxin binding sites on mammalian skeletal muscle developing in vivo, J. Physiol., 267:195.

    Google Scholar 

  • Blackshaw, S. and Warner, A., 1976, Onset of acetylcholine sensitivity and endplate activity in developing myotome muscles of Xenopus, Nature, 262:217.

    Article  Google Scholar 

  • Brehm, P., Bates, L., Kream, R. and Moody-Corbett, F., 1985, Inhibition of acetylcholine receptor incorporation blocks developmental changes in channel gating in Xenopus muscle, Soc. Neurosci. Abstracts, 11:849.

    Google Scholar 

  • Brehm, P., Kidokoro, Y., and Moody-Corbett, F., 1984a, Acetylcholine receptor channel properties during development of Xenopus muscle cells in culture, J. Physiol., 357:203.

    Google Scholar 

  • Brehm, P., Kream, R., and Moody-Corbett, F., 1987, Translational and transcriptional requirements for developmental alterations in acetylcholine receptor channel function in Xenopus myotomal muscle, Dev. Biol. (in press).

    Google Scholar 

  • Brehm, P. and Kullberg, R., 1987. Acetylcholine receptor channels on adult mouse skeletal muscle are functionally identical in synaptic and nonsynaptic membrane, Proc. Natl. Acad. Sci. USA, (in press).

    Google Scholar 

  • Brehm, P., Kullberg, R., and Moody-Corbett, F., 1984b, Properties of nonjunctional acetylcholine receptor channels on innervated muscle of Xenopus laevis, J. Physiol., 350:631.

    Google Scholar 

  • Brehm, P., Steinbach, J.H. and Kidokoro, Y., 1982, Channel open time of acetylcholine receptors on Xenopus muscle cells in dissociated cell culture, Dev. Biol., 91:93.

    Article  Google Scholar 

  • Brenner, H.R., Meier, Th., and Widmer, B., 1983, Early action of nerve determines motor endplate differentiation in rat muscle, Nature, 536.

    Google Scholar 

  • Brenner, H. and Sakmann, B., 1983, Neurotrophic control of channel properties at neuromuscular synapses of rat muscle, J. Physiol., 337:159.

    Google Scholar 

  • Brockes, J. and Hall, Z., 1975, Synthesis of acetylcholine receptor by denervated rat diaphragm muscle. Proc. Natl. Acad. Sci. USA, 72:1368.

    Article  Google Scholar 

  • Carlson, C.G., Leonard, R.J. and Nakajima, S., 1985, The aneural development of the acetylcholine receptor in the presence of agents which block protein synthesis and glycosylation. Soc. Neurosci. Abstracts, 11:156.

    Google Scholar 

  • Chow, I. and Cohen, M.W., 1983, Developmental changes in the distribution of acetylcholine receptors on the myotomes of Xenopus laevis. J. Physiol., 339:553.

    Google Scholar 

  • Cull-Candy, S.G., Miledi, R. and Uchitel, O.D., 1982, Properties of junctional and extrajunctional acetylcholine-receptor channels in organ cultured human muscle fibres, J. Physiol., 333:251.

    Google Scholar 

  • Frank, E., Jansen, J.K., Lomo, T., and Westgaard, R.H., 1975, The interaction between foreign and original nerves innervating the soleus muscle of rats, J. Physiol., 247:725.

    Google Scholar 

  • Henderson, L.P., Lechleiter, J. and Brehm, P. 1987, Single channel properties of newly synthesized acetylcholine receptors following denervation of mammalian skeletal muscle, J. Gen. Physiol., (in press).

    Google Scholar 

  • Katz, B. and Miledi, R., 1972, The statistical nature of the acetylcholine potential and its molecular components, J. Physiol., 244:703.

    Google Scholar 

  • Kullberg, R.W., Brehm, P. and Steinbach, J.H., 1981, Nonjunctional acetylcholine receptor channel open time decreases during development of Xenopus muscle. Nature, 289:411.

    Article  Google Scholar 

  • Kullberg, R. and Kasprzak, H., 1985, Gating kinetics of nonjunctional acetylcholine receptor channels in developing Xenopus muscle. J. Neurosci., 5:970.

    Google Scholar 

  • Kullberg, R.W., Lentz, T., and Cohen, M., 1977, Development of myotomal neuromuscular junction in Xenopus laevis: an electrophysiological and fine structural study, Dev. Biol., 60:101.

    Article  Google Scholar 

  • Kullberg, R.W., Mikelberg, F.S. and Cohen, M.W., 1980, Contribution of Cholinesterase to developmental decreases in the time course of synaptic potentials at an amphibian neuromuscular junction, Dev. Biol., 75:255.

    Article  Google Scholar 

  • Kullberg, R. and Owens, J.L., 1986, Comparative development of endplate currents in two muscles of Xenopus laevis, J. Physiol., 374:413.

    Google Scholar 

  • Kullberg, R., Owens, J.L. and Brehm, P., 1986, Development of nicotinic acetylcholine receptor function, Proc. Eighth Annual Conference of IEEE in Medicine and Biology 8:948.

    Google Scholar 

  • Leonard, R.J., Nakajima, S., Nakajima, Y., and Takahashi, T., 1984, Differential development of two classes of acetylcholine receptors in Xenopus muscle in culture, Science, 226:55.

    Article  Google Scholar 

  • Lomo, T. and Rosenthal, J., 1972, Control of ACh sensitivity by muscle activity in the rat, J. Physiol., 221:493.

    Google Scholar 

  • Merlie, J.P., Isenberg, K.E., Russell, S.D. and Sanes, J.R., 1984, Denervation supersensitivity in skeletal muscle: analysis with a cloned cDNA probe, J. cell. Biol., 99:332.

    Article  Google Scholar 

  • Michler, A. and Sakmann, B., 1980, Receptor stability and channel conversion in the subsynaptic membrane of the developing neuromuscular junction, Devel. Biol., 80:1.

    Article  Google Scholar 

  • Mishina, M., Takai, T., Imoto, K., Noda, M., Takahashi, T., Numa, S., Methfessel, C. and Sakmann, B., 1986, Molecular distinction between fetal and adult forms of muscle acetylcholine receptor, Nature, 321:406.

    Article  Google Scholar 

  • Neher, E. and Sakmann, B., 1976, Noise analysis of drug induced voltage clamp currents in denervated frog muscle fibres, J. Physiol., 258:705.

    Google Scholar 

  • Owens, J.L. and Kullberg, R., 1986, Development of acetylcholine receptor channel function in vivo, Soc. Neurosci. Abstr., 12:546.

    Google Scholar 

  • Schuetze, S.M., 1980, The acetylcholine channel open time in chick muscle is not decreased following innervation, J. Physiol., 303:111.

    Google Scholar 

  • Schuetze, S.M. and Role, L.W., 1987, Developmental regulation of the nicotinic acetylcholine receptor, Ann. Rev. Neurosci., (in press).

    Google Scholar 

  • Schuetze, S. and Vicini, S., 1986, Apparent acetylcholine receptor channel conversion at individual rat soleus endplates in vitro, J. Physiol., 375:153.

    Google Scholar 

  • Siegelbaum, S., Trautmann, A. and Koenig, J., 1984, Single acetylcholine-activated channel currents in developing muscle cells, Dev. Biol., 104:366.

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Dept. of Physiology, Tufts Medical School, Boston, MA, 02111, USA

    Paul Brehm, James Lechleiter & Leslie Henderson

  2. Biology Department, University of Alaska, Anchorage, AK, 99508, USA

    Jesse Owens & Richard Kullberg

Authors
  1. Paul Brehm
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  2. James Lechleiter
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  3. Leslie Henderson
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  4. Jesse Owens
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  5. Richard Kullberg
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Editor information

Editors and Affiliations

  1. UCLA School of Medicine, Los Angeles, California, USA

    Alan D. Grinnell

  2. National Institute of Environmental Health Sciences, Research Triangle, North Carolina, USA

    David Armstrong

  3. University of California, Los Angeles, Los Angeles, California, USA

    Meyer B. Jackson

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© 1988 Plenum Press, New York

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Brehm, P., Lechleiter, J., Henderson, L., Owens, J., Kullberg, R. (1988). Development and Regulation of Acetylcholine Receptor Function. In: Grinnell, A.D., Armstrong, D., Jackson, M.B. (eds) Calcium and Ion Channel Modulation. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0975-8_28

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  • DOI: https://doi.org/10.1007/978-1-4613-0975-8_28

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8273-0

  • Online ISBN: 978-1-4613-0975-8

  • eBook Packages: Springer Book Archive

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