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Separation and Biochemical Characterization of Rat Liver Parenchymal Cell Subpopulations

  • Pablo Steinberg
  • Beate Seibert
  • Franz Oesch
Conference paper

Abstract

Parenchymal cells within the hepatic lobules of the rat are morphologically and biochemically heterogeneous (Shank et al., 1959; Novikoff, 1959; Loud, 1968; Jungermann and Katz, 1982). It has been previously shown that the concentration of cytochrome P-450 and the activities of most cytochrome P-450-dependent monooxygenases are relatively higher in the centrilobular regions than in the periportal regions of the rat liver (Baron et al., 1978; Gooding et al., 1978; Baron and Kawabata, 1983); further, these studies revealed that pretreatment of the animals with phenobarbital intensified this gradation across the liver lobule, whereas after administration of 3-methylcholanthrene the concentration of cytochrome P-450 and the monooxygenase activities were more uniformly distributed. Since very reactive and toxic metabolites are often formed during cytochrome P-450-catalyzed monooxygenations of hepatotoxins, the intralobular distribution of different cytochromes P-450 may be a crucial factor in determining the location of the damage within the hepatic lobules after exposure to several hepatotoxic compounds.

Keywords

Parenchymal Cell Hepatic Lobule Liver Parenchymal Cell Centrifugal Elutriation Periportal Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Pablo Steinberg
    • 1
  • Beate Seibert
    • 1
  • Franz Oesch
    • 1
  1. 1.Institute of ToxicologyUniversity of MainzMainzWest Germany

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