Autoradiographic Localization of Brain Peptide Receptors at the Electron Microscopic Level

  • A. Beaudet
  • E. Hamel
  • K. Leonard
  • M. Vial
  • E. Moyse
  • P. Kitabgi
  • J. P. Vincent
  • W. Rostène


Neurotransmitters and related drugs exert their biochemical, electrophysiological, and, ultimately, behavioral effects by acting upon specific receptor molecules embedded in neuronal and, in some cases, perhaps also glial plasma membranes. This interaction involves: (1) recognition of a specific binding site on the surface of the receptor and (2) translation of the recognition information into a response signal. Pioneering studies by Clark (1933) demonstrated that the binding of drugs (ligands) to receptors was reversible, obeyed the law of mass action, and occurred at very low concentrations of ligand. Considerable progress in our understanding of receptor mechanisms was later to emerge from the development of sensitive methods for measuring the receptor-specific binding of radiolabeled ligand probes (Snyder and Bennett, 1976). Not only did the availability of such methods greatly facilitate the biophysical, pharmacological, and molecular characterization of receptors for neurotransmitters, but it also made possible their localization in situ, using autoradiographic techniques.


Substantia Nigra Opioid Receptor Axon Terminal Opiate Receptor Electron Microscopic Level 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • A. Beaudet
    • 1
  • E. Hamel
    • 1
  • K. Leonard
    • 1
  • M. Vial
    • 2
  • E. Moyse
    • 1
  • P. Kitabgi
    • 3
  • J. P. Vincent
    • 3
  • W. Rostène
    • 2
  1. 1.Montreal Neurological InstituteMcGill UniversityMontrealCanada
  2. 2.INSERM U-55, Hôpital St. AntoineParisFrance
  3. 3.Centre de Biochimie du CNRSUniversité de NiceNice CedexFrance

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