Skip to main content
SpringerLink
Log in

Fibronectin and Its Relation to Cellular Structure and Behavior

  • Chapter
Cell Biology of Extracellular Matrix

Abstract

Apart from proteoglycans and collagens, the best understood constituent of the extracellular matrix is fibronectin, a large glycoprotein. The structural properties of fibronectin are reviewed in Chapter 4 as well as in several recent reviews (Mosher, 1980; Yamada et al., 1980; Hynes, 1981). In this chapter we will be concerned with functional aspects of the interaction of fibronectin with cells. Current research reviewed here and in Chapter 4 places fibronectin at the interface between cells and the matrix and implicates it in several features of cellular behavior. Because almost all the information concerning functions of fibronectin comes from studies in vitro, this chapter will discuss first the distribution of fibronectin in cell culture and its effects on cellular functions in vitro. We will then consider the distribution of fibronectin in vivo and end with a rather speculative discussion of the possible functions of fibronectin in vivo.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Albrecht-Buehler, G., 1977, The phagokinetic tracks of 3T3 cells, Cell 11:395–404.

    Google Scholar 

  • Ali, I. U., and Hynes, R. O., 1977, Effects of cytochalasin B and colchicine on attachment of a major surface protein of fibroblasts, Biochim. Biophys. Acta 471:16–24.

    Google Scholar 

  • Ali, I. U., and Hynes, R. O., 1978a, Role of disulfide bonds in the attachment and function of large, external, transformation-sensitive glycoprotein at the cell surface, Biochim. Biophys. Acta 510:140–150.

    Google Scholar 

  • Ali, I. U., and Hynes, R. O., 1978b, Effects of LETS glycoprotein on cell motility, Cell 14:439–446.

    Google Scholar 

  • Ali, I. U., Mautner, V. M., Lanza, R. P., and Hynes, R. O., 1977, Restoration of normal morphology, adhesion and cytoskeleton in transformed cells by addition of a transformation-sensitive surface protein, Cell 11:115–126.

    Google Scholar 

  • Alitalo, K., Hovi, T., and Vaheri, A., 1980a, Fibronectin is produced by human macrophages, J. Exp. Med. 151:602–613.

    Google Scholar 

  • Alitalo, K., Kurkinen, M., Vaheri, A., Krieg, T., and Timpl, R., 1980b, Extracellular matrix components synthesized by human amniotic epithelial cells in culture, Cell 19:1053–1062.

    Google Scholar 

  • Arneson, M. A., Hammerschmidt, D. E., Furcht, L. T., and King, R. A., 1980, Fibronectin-correctable platelet function defect in Ehlers-Danlos syndrome, J. Am. Med. Assoc., 244:144–147.

    Google Scholar 

  • Asch, B. B., Kamat, B. R., and Burstein, N. A., 1981, Comparison of fibronectin deposition in normal, dysplastic, and malignant mammary tissues and cell cultures, Cancer Res., in press.

    Google Scholar 

  • Bell, P. B., 1977, Locomotory behavior, contact inhibition, and pattern formation of 3T3 and polyoma virus-transformed 3T3 cells in culture, J. Cell Biol. 74:963–982.

    Google Scholar 

  • Benecke, B. J., Ben-Ze’ev, A., and Penman, S., 1978, The control of mRNA production, translation, and turnover in suspended and reattached anchorage-dependent fibroblasts, Cell 14:931–939.

    Google Scholar 

  • Bensusan, H. B., Koh, T. L., Henry, K. G., Murray, B. A., and Culp, L. A., 1978, Evidence that fibronectin is the collagen receptor on platelet membranes, Proc. Natl. Acad. Sci. USA 75:5864–5868.

    Google Scholar 

  • Ben-Ze’ev, A., Farmer, S. R., and Penman, S., 1980, Protein synthesis requires cell-surface contact while nuclear events respond to cell shape in anchorage-dependent fibroblasts, Cell 21:365–372.

    Google Scholar 

  • Berlin, R. D., and Oliver, J. M., 1978, Analogous ultrastructure and surface properties during capping and phagocytosis in leukocytes, J. Cell Biol. 77:789–804.

    Google Scholar 

  • Birdwell, C. R., Gospodarowicz, D., and Nicolson, G. L., 1978, Identification, localization and role of fibronectin in cultured bovine endothelial cells, Proc. Natl. Acad. Sci. USA 75:3273–3277.

    Google Scholar 

  • Blumenstock, F. A., Saba, T. M., Weber, P., and Laffin, R., 1978, Biochemical and immunological characterization of human opsonic α 2SB glycoprotein: Its identity with cold-insoluble globulin, J. Biol. Chem. 253:4287–4291.

    Google Scholar 

  • Bon, S., Vigny, M., and Massoulié, J., 1979, Asymmetric and globular forms of acetylcholinesterase in mammals and birds, Proc. Natl. Acad. Sci. USA 76:2546–2550.

    Google Scholar 

  • Burridge, K., and Feramisco, J., 1980, Microinjection and localization of a 130K protein in living fibroblasts: A relationship to actin and fibronectin, Cell 19:587–595.

    Google Scholar 

  • Carter, S. B., 1967, Haptotaxis and the mechanism of cell motility, Nature (London) 213:256–260.

    Google Scholar 

  • Chen, L. B., 1977, Alteration in cell surface LETS protein during myogenesis, Cell 10:393–400.

    Google Scholar 

  • Chen, L. B., Gallimore, P. H., and McDougall, J. K., 1976, Correlation between tumor induction and the large external transformation-sensitive protein on the cell surface, Proc. Natl. Acad. Sci. USA 73:3570–3574.

    Google Scholar 

  • Chen, L. B., Maitland, N., Gallimore, P. H., and McDougall, J. K., 1977, Detection of the large external transformation-sensitive protein on some epithelial cells, Exp. Cell Res. 106:39–46.

    Google Scholar 

  • Chen, L. B., Murray, A., Segal, R. A., Bushnell, A., and Walsh, M. L., 1978, Studies on intercellular LETS glycoprotein matrices, Cell 14:377–391.

    Google Scholar 

  • Chen, L. B., Summerhayes, I., Hsieh, P., and Gallimore, P. H., 1980, Possible role of fibronectin in malignancy, in: Tumor Cell Surfaces and Malignancy (R. O. Hynes and C. F. Fox, eds.), pp. 505–516, Liss, New York.

    Google Scholar 

  • Chiquet, M., Puri, E. C., and Turner, D. C., 1979, Fibronectin mediates attachment of chicken myoblasts to a gelatin-coated substratum, J. Biol. Chem. 254:5475–5482.

    Google Scholar 

  • Colvin, R. I., Lanigan, J., Clark, R. A., Ebert, T. H., Verderber, E., and Hammond, M. E., 1979, Macrophage fibronection (cold-insoluble globulin, LETS protein), Fed. Proc. 38:1408.

    Google Scholar 

  • Coon, H. C., 1966, Clonal stability and phenotypic expression of chick cartilage cells in vitro, Proc. Natl. Acad. Sci. USA 55:66–73.

    Google Scholar 

  • Couchman, J. R., Gibson, W. T., Thorn, D., Weaver, A. C., Rees, D. A., and Parish, W. E., 1979, Fibronectin distribution in epithelial and associated tissues of the rat, Arch. Dermatol. Res. 266:295–310.

    Google Scholar 

  • Courtoy, P. J., Kanwar, Y. S., Hynes, R. O., and Farquhar, M. G., 1980, Fibronectin localization in the rat glomerulus, J. Cell Biol. 87:691–696.

    Google Scholar 

  • Critchley, D. R., England, M. A., Wakely, J., and Hynes, R. O., 1979, Distribution of fibronectin in the ectoderm of gastrulating chick embryos, Nature (London) 280:498–500.

    Google Scholar 

  • Crouch, E., Balian, G., Holbrook, K., Duksin, D., and Bornstein, P., 1978, Amniotic fluid fibronectin. Characterization and synthesis by cells in culture, J. Cell Biol 78:701–715.

    Google Scholar 

  • Culp, L. A., Murray, B. A., and Rollins, B. J., 1980, Fibronectin and proteoglycans as determinants of cell-substratum adhesion, in: Tumor Cell Surfaces and Malignancy (R. O. Hynes and C. F. Fox, eds.), pp. 281–307, Liss, New York.

    Google Scholar 

  • Der, C. J., and Stanbridge, E. J., 1978, Lack of correlation between the decreased expression of cell surface LETS protein and tumorigenicity in human cell hybrids, Cell 15:1241–1251.

    Google Scholar 

  • Dessau, W., Sasse, J., Timpl, R., Jilek, F., and von der Mark, K., 1978, Synthesis and extracellular deposition of fibronectin in chondrocyte cultures, J. Cell Biol. 79:342–355.

    Google Scholar 

  • Doran, J. E., Mansberger, A. R., and Pease, A. C., 1980, Cold-insoluble globulin-enhanced phagocytosis of gelatinized targets by macrophage monolayers: A model system, J. Reticuloendothelial Soc. 27:471–483.

    Google Scholar 

  • Emmerling, M. R., Johnson, C. D., Mosher, D. F., Lipton, B. H., and Lilien, J. E., 1981, Crosslinking and binding of fibronectin with asymmetric acetylcholinesterase, Biochemistry, 20:3242–3247.

    Google Scholar 

  • Engvall, E., and Ruoslahti, E., 1977, Binding of soluble form of fibroblast surface protein, fibronectin, to collagen, Int. J. Cancer 20:1–5.

    Google Scholar 

  • Erickson, C. A., Tosney, K. W., and Weston, J. A., 1980, Analysis of migratory behavior of neural crest and fibroblastic cells in embryonic tissues, Dev. Biol. 77:142–156.

    Google Scholar 

  • Farmer, S. R., Ben-Ze’ev, A., Benecke, B. J., and Penman, S., 1978, Altered translatability of messenger RNA from suspended anchorage-dependent fibroblasts: Reversal upon cell attachment to a surface, Cell 15:627–637.

    Google Scholar 

  • Folkman, J., and Moscona, A., 1978, Role of cell shape in growth control, Nature (London) 273:345–349.

    Google Scholar 

  • Furcht, L. T., Mosher, D. F., and Wendelschafer-Crabb, G., 1978, Immunocytochemical localization of fibronectin (LETS protein) on the surface of L6 myoblasts: Light and electron microscopic studies, Cell 13:263–271.

    Google Scholar 

  • Gail, M. H., and Boone, C. W., 1970, The locomotion of mouse fibroblasts in tissue culture, Biophys. J. 10:980–993.

    Google Scholar 

  • Gauss-Müller, V., Kleinman, H. K., Martin, G. R., and Schiffman, E., 1980, Role of attachment and attractants in fibroblast Chemotaxis, J. Lab. Clin. Med. 96:1071–1080.

    Google Scholar 

  • Geiger, B., Tokuyasu, K. T., Dutton, A. H., and Singer, S. J., 1980, Vinculin: An intracellular protein localized at specific sites where microfilament bundles terminate at cell membranes, Proc. Natl Acad. Sci. USA 77:4127–4131.

    Google Scholar 

  • Ginsberg, M. H., Painter, R. G., Forsyth, J., Birdwell, C., and Plow, E. F., 1980, Thrombin increases expression of fibronectin antigen on the platelet surface, Proc. Natl Acad. Sci. USA 77:1049–1053.

    Google Scholar 

  • Goldman, R. D., Yerna, M. J., and Schloss, J. A., 1977, Localization and organization of microfilaments and related proteins in normal and virus-transformed cells, in: Cell Shape and Surface Architecture (J.-P. Revel, U. Henning, and C. F. Fox, eds.), pp. 107–135, Liss, New York.

    Google Scholar 

  • Gospodarowicz, D., Greenburg, G., and Birdwell, C. R., 1978, Determination of cellular shape by the extracellular matrix and its correlation with the control of cellular growth, Cancer Res. 38:4155–4171.

    Google Scholar 

  • Gospodarowicz, D., Delgado, D., and Vlodavsky, I., 1980, Permissive effect of the extracellular matrix on cell proliferation, Proc. Natl. Acad. Sci. USA 77:4094–4098.

    Google Scholar 

  • Graham, J. M., Hynes, R. O., Rowlatt, C., and Sandali, J. K., 1978, Cell surface coat of hamster fibroblasts, Ann. N. Y. Acad. Sci. 312:221–239.

    Google Scholar 

  • Griffin, F. M., Griffin, J. A., and Silverstein, S. C., 1976, Studies on the mechanism of phagocytosis. II. The interaction of macrophages with anti-IgG-coated bone marrow derived lymphocytes, J. Exp. Med. 144:788–809.

    Google Scholar 

  • Grinnell, F., 1978, Cellular adhesiveness and extracellular substrata, Int. Rev. Cytol. 53:65–144.

    Google Scholar 

  • Grinnell, F., 1980, Fibroblast receptor for cell-substratum adhesion: Studies on the interaction of baby hamster kidney cells with latex beads coated by cold insoluble globulin (plasma fibronectin), J. Cell Biol. 86:104–112.

    Google Scholar 

  • Grinnell, F., and Feld, M. K., 1979, Initial adhesion of human fibroblasts in serum-free medium: Possible role of secreted fibronectin, Cell 17:117–129.

    Google Scholar 

  • Grinnell, F., and Hays, D. G., 1978, Cell adhesion and spreading factor: Similarity to cold insoluble globulin in human serum, Exp. Cell Res. 115:221–229.

    Google Scholar 

  • Grinnell, F., Feld, M., and Snell, W., 1979, The influence of cold insoluble globulin on platelet morphological response to substrata, Cell Biol. Int. Rep. 3:585–592.

    Google Scholar 

  • Grinnell, F., Feld, M., and Minter, D., 1980, Fibroblast adhesion to fibrinogen and fibrin substrata: Requirement for cold-insoluble globulin (plasma fibronectin), Cell 19:517–525.

    Google Scholar 

  • Gudewicz, P. W., Molnar, J., Lai, M. Z., Beezhold, D. W., Siefring, G. E., Credo, R. B., and Lorand, L., 1980, Fibronectin-mediated uptake of gelatin-coated latex particles by peritoneal macrophages, J. Cell Biol. 87:427–433.

    Google Scholar 

  • Hassell, J. R., Pennypacker, J. P., Kleinman, H. K., Pratt, R. M., and Yamada, K. M., 1979, Enhanced cellular fibronectin accumulation in chondrocytes treated with vitamin A, Cell 17:821–826.

    Google Scholar 

  • Hayman, E. G., and Ruoslahti, E., 1979, Distribution of fetal bovine serum fibronectin and endogenous rat cell fibronectin in extracellular matrix, J. Cell Biol. 83:255–259.

    Google Scholar 

  • Hedman, K., Vaheri, A., and Wartiovaara, J., 1978, External fibronectin of cultured human fibroblasts is predominantly a matrix protein, J. Cell Biol. 76:748–760.

    Google Scholar 

  • Hedman, K., Kurkinen, M., Alitalo, K., Vaheri, A., Johannson, S., and Höök, M., 1979, Isolation of the pericellular matrix of human fibroblast cultures, J. Cell Biol. 81:83–91.

    Google Scholar 

  • Heggeness, M. H., Ash, J. F., and Singer, S. J., 1978, Transmembrane linkage of fibronectin to intracellular actin-containing filaments in cultured human fibroblasts, Ann. N.Y. Acad. Sci. 312:414–417.

    Google Scholar 

  • Hewitt, A. T., Kleinman, H. K., Pennypacker, J. P., and Martin, G. R., 1980, Identification of an adhesive factor for chondrocytes, Proc. Natl. Acad. Sci. USA 77:385–388.

    Google Scholar 

  • Hogan, B. L. M., 1980, High molecular weight extracellular proteins synthesized by endoderm cells derived from mouse teratocarcinoma cells and normal extraembryonic membranes, Dev. Biol. 76:275–285.

    Google Scholar 

  • Höök, M., Rubin, K., Oldberg, A., Obrink, B., and Vaheri, A., 1977, Cold-insoluble globulin mediates the adhesion of rat liver cells to plastic petri dishes, Biochem. Biophys. Res. Commun. 79:726–733.

    Google Scholar 

  • Hynes, R. O., 1976, Cell surface proteins and malignant transformation, Biochim. Biophys. Acta 458:73–107.

    Google Scholar 

  • Hynes, R. O., (ed.), 1979, Proteins and glycoproteins, in: Surfaces of Normal and Malignant Cells, pp. 103–148, Wiley, New York.

    Google Scholar 

  • Hynes, R. O., 1981, Relationships between fibronectin and the cytoskeleton, in: Cell Surface Reviews, Vol. 7 (G. Poste and G. L. Nicolson, eds.), pp. 97–136, Elsevier/North-Holland, Amsterdam.

    Google Scholar 

  • Hynes, R. O., and Destree, A. T., 1977, Extensive disulfide bonding at the mammalian cell surface, Proc. Natl. Acad. Sci. USA 74:2855–2859.

    Google Scholar 

  • Hynes, R. O., and Destree, A. T., 1978, Relationships between fibronectin (LETS protein) and actin, Cell 15:875–886.

    Google Scholar 

  • Hynes, R. O., Martin, G. S., Critchley, D. R., Shearer, M., and Epstein, C. J., 1976, Viral transformation of rat myoblasts: Effects on fusion and surface properties, Dev. Biol. 48:35–46.

    Google Scholar 

  • Hynes, R. O., Ali, I. U., Destree, A. T., Mautner, V., Perkins, M. E., Senger, D. R., Wagner, D. D., and Smith, K. K., 1978, A large glycoprotein lost from the surfaces of transformed cells, Ann. N.Y. Acad. Sci. 312:317–342.

    Google Scholar 

  • Hynes, R. O., Destree, A. T., Perkins, M. E., and Wagner, D. D., 1979, Cell surface fibronectin and oncogenic transformation, J. Supramol. Struct. 11:95–104.

    Google Scholar 

  • Isliker, H., Bing, D. H., Lahav, J., and Hynes, R. O., 1981, Interactions of fibronectin with C1q, a subcomponent of complement, Niels Jerne Festschrift, in press.

    Google Scholar 

  • Jaffe, E. A., and Mosher, D. F., 1978, Synthesis of fibronectin by cultured human endothelial cells, J. Exp. Med. 147:1779–1791.

    Google Scholar 

  • Jilek, F., and Hörmann, H., 1978, Fibronectin (cold-insoluble globulin). V. Mediation of fibrin-monomer binding to macrophages, Hoppe-Seyler’s Z. Physiol. Chem. 359:1603–1605.

    Google Scholar 

  • Jilek, F., and Hörmann, H., 1979, Fibronectin (cold insoluble globulin). VI. Influence of heparin and hyaluronic acid on the binding of native collagen, Hoppe-Seyler’s Z. Physiol. Chem. 360:597–603.

    Google Scholar 

  • Johansson, S., Rubin, K., Höök, M., Ahlgren, T., and Seljelin, R., 1979, In vitro biosynthesis of cold insoluble globulin (fibronectin) by mouse peritoneal macrophages, FEBS Lett. 105:313–316.

    Google Scholar 

  • Kahn, P., and Shin, S. I., 1979, Cellular tumorigenicity in nude mice. Test of associations among loss of cell-surface fibronectin, anchorage independence, and tumor-forming ability, J. Cell Biol. 82:1–16.

    Google Scholar 

  • Keski-Oja, J., Mosher, D. F., and Vaheri, A., 1976, Cross-linking of a major fibroblast surface-associated glycoprotein (fibronectin) catalyzed by blood coagulation factor XIII, Cell 9:29–35.

    Google Scholar 

  • Keski-Oja, J., Mosher, D. F., and Vaheri, A., 1977, Dimeric character of fibronectin, a major cell surface-associated glycoprotein, Biochem. Biophys. Res. Commun. 74:699–706.

    Google Scholar 

  • Killen, P. D., and Striker, G. E., 1979, Human glomerular visceral epithelial cells synthesize a basal lamina collagen in vitro, Proc. Natl. Acad. Sci. USA 76:3518–3522.

    Google Scholar 

  • Klebe, R. J., 1974, Isolation of a collagen-dependent cell attachment factor, Nature (London) 250:248–251.

    Google Scholar 

  • Kleinman, H. K., McGoodwin, E. B., Rennard, S. I., and Martin, G. R., 1979a, Preparation of collagen substrates for cell attachment: Effect of collagen concentration and phosphate buffer, Anal. Biochem. 94:308–312.

    Google Scholar 

  • Kleinman, H. K., Martin, G. R., and Fishman, P. H., 1979b, Ganglioside inhibition of fibronectin-mediated cell adhesion to collagen, Proc. Natl. Acad. Sci. USA 76:3367–3371.

    Google Scholar 

  • Krieg, T., Timpl, R., Alitalo, K., Kurkinen, M., and Vaheri, A., 1979, Type III procollagen is the major collagenous component produced by a continuous rhabdomyosarcoma cell line, FEBS Lett 104:405–409.

    Google Scholar 

  • Kurkinen, M., and Alitalo, K., 1979, Fibronectin and procollagen produced by a clonal line of Schwann cells, FEBS Lett. 102:64–68.

    Google Scholar 

  • Kurkinen, M., Wartiovaara, J., and Vaheri, A., 1978, Cytochalasin B releases a major surface-associated glycoprotein, fibronectin, from cultured fibroblasts, Exp. Cell Res. 111:127–137.

    Google Scholar 

  • Kurkinen, M., Alitalo, K., Vaheri, A., Stenman, S., and Saxen, L., 1979, Fibronectin in the development of embryonic chick eye, Dev. Biol. 69:589–600.

    Google Scholar 

  • Kuusela, P., 1978, Fibronectin binds to Staphylococcus aureus, Nature (London) 276:718–720.

    Google Scholar 

  • Kuusela, P., Vaheri, A., Palo, J., and Ruoslahti, E., 1978, Demonstration of fibronectin in human cerebrospinal fluid, J. Lab. Clin. Med. 92:595–601.

    Google Scholar 

  • Linder, E., Vaheri, A., Ruoslahti, E., and Wartiovaara, J., 1975, Distribution of fibroblast surface antigen in the developing chick embryo, J. Exp. Med. 142:41–49.

    Google Scholar 

  • Linder, E., Stenman, S., Lehto, V. P., and Vaheri, A., 1978, Distribution of fibronectin in human tissues and relationship to other connective tissue components, Ann. N. Y. Acad. Sci. 312:151–159.

    Google Scholar 

  • Löfberg, J., Ahlfors, K., and Fällström, C., 1980, Neural crest cell migration in relation to extracellular matrix organization in the embryonic axolotl trunk, Dev. Biol. 75:148–167.

    Google Scholar 

  • McGuire, J. C., Greene, L. A., and Furano, A. V., 1978, NGF stimulates incorporation of fucose or glucosamine into an external glycoprotein in cultured rat PC12 pheochromocytoma cells, Cell 15:357–365.

    Google Scholar 

  • McMahan, U. J., Sanes, J. R., and Marshall, L. M., 1978, Cholinesterase is associated with the basal lamina at the neuromuscular junction, Nature (London) 271:172–174.

    Google Scholar 

  • Macarak, E. J., Kirby, E., Kirk, T., and Kefalides, N. A., 1978, Synthesis of cold-insoluble globulin by cultured calf endothelial cells, Proc. Natl. Acad. Sci. USA 75:2621–2625.

    Google Scholar 

  • Matsuda, M., Yoshida, N., Aoki, N., and Wakabayashi, K., 1978, Distribution of cold-insoluble globulin in plasma and tissues, Ann. N.Y. Acad. Sci. 312:74–92.

    Google Scholar 

  • Mautner, V., and Hynes, R. O., 1977, Surface distribution of LETS protein in relation to the cytoskeleton of normal and transformed cells, J. Cell Biol. 75:743–758.

    Google Scholar 

  • Mayer, B. W., Hay, E. D., and Hynes, R. O., 1981, Immunocytochemical localization of fibronectin in embryonic chick trunk and area vasculosa, Dev. Biol. 82:267–286.

    Google Scholar 

  • Michl, J., Pieczonka, M. M., Unkeless, J. C., and Silverstein, S. C., 1979, Effects of immobilized immune complexes of Fc and complement receptor function in resident and thioglycollate-elicited mouse peritoneal macrophages, J. Exp. Med. 150:607–621.

    Google Scholar 

  • Molnar, J., Gelder, F. B., Lai, M. Z., Siefring, G. E., Credo, R. B., and Lorand, L., 1979, Purification of opsonically active human and rat cold-insoluble globulin (plasma fibronectin), Biochemistry 18:3909–3916.

    Google Scholar 

  • Mosher, D. F., 1976, Action of fibrin-stabilizing factor on cold-insoluble globulin and α2-macroglobulin in clotting plasma, J. Biol. Chem. 251:1639–1645.

    Google Scholar 

  • Mosher, D. F., 1980, Fibronectin, Progr. Hemostasis Thromb. 5:111–151.

    Google Scholar 

  • Mosher, D. F., and Proctor, R. A., 1980, Binding and factor XIIIa-mediated crosslinking of a 27-kilodalton fragment of fibronectin to Staphylococcus aureus, Science 209:927–929.

    Google Scholar 

  • Mosher, D. F., Saksela, O., Keski-Oja, J., and Vaheri, A., 1977, Distribution of a major surface-associated glycoprotein, fibronectin, in cultures of adherent cells, J. Supramol. Struct. 6:551–557.

    Google Scholar 

  • Mosher, D. F., Schad, P. E., and Kleinman, H. K., 1979, Cross-linking of fibronectin to collagen by blood coagulation factor XIIIa, J. Clin. Invest. 64:781–787.

    Google Scholar 

  • Newman, S. A., and Frisch, H. L., 1979, Dynamics of skeletal pattern formation in developing chick limb, Science 205:662–668.

    Google Scholar 

  • O’Neill, C. H., Riddle, P. N., and Jordan, P. W., 1979, The relation between surface area and anchorage dependence of growth in hamster and mouse fibroblasts, Cell 16:909–918.

    Google Scholar 

  • Orly, J., and Sato, G., 1979, Fibronectin mediates cytokinesis and growth of rat follicular cells in serum-free medium, Cell 17:295–305.

    Google Scholar 

  • Pearlstein, E., 1976, Plasma membrane glycoprotein which mediates adhesion of fibroblasts to collagen, Nature (London) 262:497–500.

    Google Scholar 

  • Pearlstein, E., Dienstman, S. R., and Defendi, V., 1978, Identification of macrophage external membrane proteins and their possible role in cell adhesion, J. Cell Biol. 79:263–267.

    Google Scholar 

  • Pena, S. D. J., and Hughes, R. C., 1978, Fibroblast to substratum contacts mediated by the different forms of fibronectin, Cell Biol. Int. Rep. 2:339–344.

    Google Scholar 

  • Pennypacker, J. P., Hassell, J. R., Yamada, K. M., and Pratt, R. M., 1979, The influence of an adhesive cell surface protein on chondrogenic expression in vitro, Exp. Cell Res. 121:411–415.

    Google Scholar 

  • Perkins, M. E., Ji, T. H., and Hynes, R. O., 1979, Crosslinking of fibronectin to sulfated proteoglycans at the cell surface, Cell 16:941–953.

    Google Scholar 

  • Plow, E. F., Birdwell, C., and Ginsberg, M. H., 1979, Identification and quantitation of platelet-associated fibronectin antigen, J. Clin. Invest. 63:540–543.

    Google Scholar 

  • Podleski, T. R., Greenberg, I., Schlessinger, J., and Yamada, K. M., 1979, Fibronectin delays the fusion of L6 myoblasts, Exp. Cell Res. 122:317–326.

    Google Scholar 

  • Pollack, R., Osborn, M., and Weber, K., 1975, Patterns of organization of actin and myosin in normal and transformed cultured cells, Proc. Natl. Acad. Sci. USA 72:994–998.

    Google Scholar 

  • Quaroni, A., Isselbacher, K. J., and Ruoslahti, E., 1978, Fibronectin synthesis by epithelial crypt cells of rat small intestine, Proc. Natl Acad. Sci. USA 75:5548–5552.

    Google Scholar 

  • Quigley, J. P., 1979, Proteolytic enzymes of normal and malignant cells, in: Surfaces of Normal and Malignant Cells (R. O. Hynes, ed.), pp. 247–285, Wiley, New York.

    Google Scholar 

  • Reaven, E. P., and Axline, S. G., 1973, Subplasmalemmal microfilaments and microtubules in resting and phagocytozing cultivated macrophages, J. Cell Biol. 59:12–27.

    Google Scholar 

  • Rizzino, A., and Crowley, C., 1980, Growth and differentiation of embryonal carcinoma cell line F9 in defined media, Proc. Natl. Acad. Sci. USA 77:457–461.

    Google Scholar 

  • Rojkind, M., Gatmaitan, Z., Mackensen, S., Giambrone, M. A., Ponce, P., and Reid, L. M., 1980, Connective tissue biomatrix: Its isolation and utilization for long-term cultures of normal rat hepatocytes, J. Cell Biol. 87:255–263.

    Google Scholar 

  • Saba, T. M., and Jaffe, E., 1980, Plasma fibronectin (opsonic glycoprotein): Its synthesis by endothelial cells and role in cardiopulmonary integrity after trauma as related to reticuloendothelial function, Am. J. Med. 68:577–594.

    Google Scholar 

  • Saba, T. M., Blumenstock, F. A., Weber, P., and Kaplan, J. E., 1978a, Physiologic role of cold-insoluble globulin in systemic host defense: Implications of its characterization as the opsonic α2-surface binding glycoprotein, Ann. N.Y. Acad. Sci. 312:43–55.

    Google Scholar 

  • Saba, T. M., Blumenstock, F. A., Scovili, W. A., and Bernard, H., 1978b, Cryoprecipitate reversal of opsonic α2-surface binding glycoprotein deficiency in septic surgical and trauma patients, Science 201:622–624.

    Google Scholar 

  • Santoro, S. A., and Cunningham, L. W., 1979, Fibronectin and the multiple interaction model for platelet-collagen adhesion, Proc. Natl. Acad. Sci. USA 76:2644–2648.

    Google Scholar 

  • Schachner, M., Schoonmaker, G., and Hynes, R. O., 1978, Cellular and subcellular localization of LETS protein in the nervous system, Brain Res. 158:149–158.

    Google Scholar 

  • Scovili, W. A., Saba, T. M., Blumenstock, F. A., Bernard, H., and Powers, S. R., 1978, Osponic α2 surface binding glycoprotein during sepsis, Ann. Surg. 183:521–529.

    Google Scholar 

  • Shin, S. I., Freedman, V. H., Risser, R., and Pollack, R., 1975, Tumorigenicity of virus-transformed cells in nude mice is correlated specifically with anchorage independent growth in vitro, Proc. Natl. Acad. Sci. USA 72:4435–4439.

    Google Scholar 

  • Silverstein, S. C., Steinman, R. M., and Cohn, Z. A., 1977, Endocytosis, Annu. Rev. Biochem. 46:669–722.

    Google Scholar 

  • Singer, I. I., 1979, The fibronexus: A transmembrane association of fibronectin-containing fibers and bundles of 5 nm microfilaments in hamster and human fibroblasts, Cell 16:675–685.

    Google Scholar 

  • Smith, H. S., Riggs, J. L., and Mosesson, M. W., 1979, Production of fibronectin (LETS protein) by human epithelial cells in culture, Cancer Res. 39:4138–4145.

    Google Scholar 

  • Spiegel, E., Burger, M., and Spiegel, M., 1980, Fibronectin in the developing sea urchin embryo, J. Cell Biol. 87:309–313.

    Google Scholar 

  • Steimer, K. S., and Klagsbrun, M., 1981, Serum-free growth of normal and transformed fibroblasts in milk: Differential requirements for fibronectin, J. Cell. Biol. 88:294–300.

    Google Scholar 

  • Stenman, S., and Vaheri, A., 1978, Distribution of a major connective tissue protein, fibronectin, in normal human tissues, J. Exp. Med. 147:1054–1064.

    Google Scholar 

  • Sugrue, S. P., and Hay, E. D., 1980, Response of basal epithelial cell surface and cytoskeleton to solubilized extracellular matrix molecules, J. Cell Biol. 87:126a.

    Google Scholar 

  • Sun, T. T., and Green, H., 1977, Cultured epithelial cells of cornea, conjunctiva, and skin: Absence of marked intrinsic divergence of their differentiated states, Nature (London) 269:489–492.

    Google Scholar 

  • Thesleff, I., Stenman, S., Vaheri, A., and Timpl, R., 1979, Changes in the matrix proteins, fibronectin and collagen, during differentiation of mouse tooth germ, Dev. Biol. 70:116–126.

    Google Scholar 

  • Thorn, D., Powell, A. J., and Rees, D. A., 1979, Mechanisms of cellular adhesion. IV. Role of serum glycoproteins in fibroblast spreading on glass, J. Cell Sci. 35:281–305.

    Google Scholar 

  • Vaheri, A., and Ruoslahti, E., 1975, Fibroblast surface antigen produced but not retained by virus-transformed human cells, J. Exp. Med. 142:530–538.

    Google Scholar 

  • Vaheri, A., and Mosher, D. F., 1978, High molecular weight, cell surface-associated glycoprotein (fibronectin) lost in malignant transformation, Biochim. Biophys. Acta 516:1–25.

    Google Scholar 

  • Vaheri, A., Ruoslahti, E., Westermark, B., and Ponten, J., 1976, A common cell-type specific surface antigen in cultured human glial cells and fibroblasts: Loss in malignant cells, J. Exp. Med. 143:64–72.

    Google Scholar 

  • Vaheri, A., Kurkinen, M., Lehto, V. P., Linder, E., and Timpl, R., 1978, Codistribution of pericellular matrix proteins in cultured fibroblasts and loss in transformation: Fibronectin and procollagen, Proc. Natl. Acad. Sci. USA 75:4944–4948.

    Google Scholar 

  • Van De Water, L., Schroeder, S., Crenshaw, E. B., and Hynes, R. O., 1981, Fibronectin promotes phagocytosis of latex particles by a murine macrophage line, J. Cell. Biol. 90:32–39.

    Google Scholar 

  • Vlodavsky, I., Johnson, L. K., Greenburg, G., and Gospodarowicz, D., 1979, Vascular endothelial cells maintained in the absence of fibroblast growth factor undergo structural and functional alterations that are incompatible with their in vivo differentiated properties, J. Cell Biol. 83:468–486.

    Google Scholar 

  • Vlodavsky, I., Lui, G. M., and Gospodarowicz, D., 1980, Morphological appearance, growth behavior and migratory activity of human tumor cells maintained on extracellular matrix versus plastic, Cell 19:607–616.

    Google Scholar 

  • Voss, B., Allam, S., and Rauterberg, J., 1979, Primary cultures of rat hepatocytes synthesize fibronectin, Biochem. Biophys. Res. Commun. 90:1348–1354.

    Google Scholar 

  • Vuento, M., Vartio, T., Saraste, M., Von Bondsorff, C. H., and Vaheri, A., 1980, Spontaneous and polyamine-induced formation of filamentous polymers from soluble fibronectin, Eur. J. Biochem. 105:33–42.

    Google Scholar 

  • Wagner, D. D., and Hynes, R. O., 1979, Domain structure of fibronectin and its relation to function: Disulfides and sulfhydryl groups, J. Biol. Chem. 254:6746–6754.

    Google Scholar 

  • Wagner, D. D., and Hynes, R. O., 1980, Topological arrangement of the major structural features of fibronectin, J. Biol. Chem. 255:4304–4312.

    Google Scholar 

  • Wagner, D. D., Ivatt, R., Destree, A. T., and Hynes, R. O., 1981, Fibronectins of normal and transformed hamster cells, J. Biol. Chem., (in press).

    Google Scholar 

  • Walsh, F. S., Moore, S. E., and Dhut, S., 1981, Monoclonal antibody to human fibronectin: Production and characterization using human muscle cultures, Dev. Biol., 84:121–132.

    Google Scholar 

  • Warburton, M. J., Head, L. P., and Rudland, P. S., 1981, Redistribution of fibronectin and cytoskeletal proteins during the differentiation of rat mammary tumor cells in vitro, Exp. Cell Res. 132:57–66.

    Google Scholar 

  • Wartiovaara, J., Leivo, I., Virtanen, I., Vaheri, A., and Graham, C. F., 1978, Appearance of fibronectin during differentiation of mouse teratocarcinoma in vitro, Nature (London) 272:355–356.

    Google Scholar 

  • Wartiovaara, J., Leivo, I., and Vaheri, A., 1979, Expression of the cell surface-associated glycoprotein, fibronectin, in the early mouse embryo, Dev. Biol. 69:247–257.

    Google Scholar 

  • Wartiovaara, J., Leivo, I., and Vaheri, A., 1980, Matrix glycoproteins in early mouse development and in differentiation of teratocarcinoma cells, in: The Cell Surface: Mediator of Developmental Processes (S. Subtelny and N. K. Wessells, eds.), pp. 305–328, Academic Press, New York.

    Google Scholar 

  • Weiss, P., 1961, Guiding principles in cell locomotion and cell aggregation, Exp. Cell Res. Suppl. 8:260–281.

    Google Scholar 

  • Weiss, R. E., and Reddi, A. H., 1980, Synthesis and localization of fibronectin during collagenous matrix-mesenchymal cell interaction and differentiation of cartilage and bone in vivo, Proc. Natl. Acad. Sci. USA 77:2074–2078.

    Google Scholar 

  • West, C. M., Lanza, R. Rosenbloom, J., Lowe, M., Holtzer, H., and Avdalovic, N., 1979, Fibronectin alters the phenotypic properties of cultured chick embryo chondroblasts, Cell 17:491–501.

    Google Scholar 

  • Willingham, M. C., Yamada, K. M., Yamada, S. S., Pouyssegur, J., and Pastan, I., 1977, Microfilament bundles and cell shape are related to adhesiveness to substratum and are dissociable from growth control in cultured fibroblasts, Cell 10:375–380.

    Google Scholar 

  • Wolfe, J., Mautner, V., Hogan, B., and Tilly, R., 1979, Synthesis and retention of fibronectin (LETS protein) by mouse teratocarcinoma cells, Exp. Cell Res. 118:63–71.

    Google Scholar 

  • Wylie, C. C., Heasman, J., Swan, A. P., and Anderton, B. H., 1979, Evidence for substrate guidance of primordial germ cells, Exp. Cell Res. 121:315–324.

    Google Scholar 

  • Yamada, K. M., and Kennedy, D. W., 1979, Fibroblast cellular and plasma fibronectins are similar but not identical, J. Cell Biol. 80:492–498.

    Google Scholar 

  • Yamada, K. M., and Olden, K., 1978, Fibronectins: Adhesive glycoproteins of cell surface and blood, Nature (London) 275:179–184.

    Google Scholar 

  • Yamada, K. M., Yamada, S. S., and Pastan, I., 1976a, Cell surface protein partially restores morphology, adhesiveness, and contact inhibition of movement to transformed fibroblasts, Proc. Natl. Acad. Sci. USA 73:1217–1221.

    Google Scholar 

  • Yamada, K. M., Ohanian, S. H., and Pastan, I., 1976b, Cell surface protein decreases microvilli and ruffles on transformed mouse and chick cells, Cell 9:241–245.

    Google Scholar 

  • Yamada, K. M., Olden, K., and Hahn, L. H. E., 1980, Cell surface protein and cell interactions, in: The Cell Surface: Mediator of Developmental Processes (S. Subtelny and N. K. Wessells, eds.), Academic Press, New York.

    Google Scholar 

  • Yang, N. S., Kirland, W., Jorgenson, T., and Furmanski, P., 1980, Absence of fibronectin and presence of plasminogen activator in both normal and malignant human mammary epithelial cells in culture, J. Cell Biol. 84:120–130.

    Google Scholar 

  • Zetter, B. R., and Martin, G. R., 1978, Expression of a high molecular weight cell surface glycoprotein (LETS protein) by preimplantation mouse embryos and teratocarcinoma stem cells, Proc. Natl. Acad. Sci. USA 75:2324–2328.

    Google Scholar 

  • Zetter, B. R., Johnson, L. K., Shuman, M. A., and Gospodarowicz, D., 1978, The isolation of vascular endothelial cell lines with altered cell surface and platelet-binding properties, Cell 14:501–509.

    Google Scholar 

  • Zucker, M. B., Mosesson, M. W., Broekman, M. J., and Kaplan, K. L., 1979, Release of platelet fibronectin (cold-insoluble globulin) from alpha granules induced by thrombin or collagen; lackof requirement for plasma fibronectin in ADP-induced platelet aggregation, Blood 54:8–12.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA

    Richard O. Hynes

Authors
  1. Richard O. Hynes
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Anatomy, Harvard Medical School, Boston, Massachusetts, 02115, USA

    Elizabeth D. Hay

Rights and permissions

Reprints and permissions

Copyright information

© 1981 Plenum Press, New York

About this chapter

Cite this chapter

Hynes, R.O. (1981). Fibronectin and Its Relation to Cellular Structure and Behavior. In: Hay, E.D. (eds) Cell Biology of Extracellular Matrix. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0881-2_11

Download citation

  • DOI: https://doi.org/10.1007/978-1-4613-0881-2_11

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8226-6

  • Online ISBN: 978-1-4613-0881-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation