Zone Evolution in Isoelectric Focusing
The isoelectric focusing process is clearly subdivided into two qualitatively different stages (see Chapter III). Directly after the electric field is turned on, the charged particles existing in the solution begin to move. Assuming that the hypothesis of local chemical equilibrium is valid, this process of migration in a field may be described as the motion of quasisubstances, which in Chapter II are called chemical subsystems and can be characterized by individual electrophoretic mobilities. Let us recall that there are two types of chemical systems: “buffers,” whose mobilities do not change sign in the electrophoretic space; and “samples,” moving to their own isoelectric point, at which their mobility changes sign.
KeywordsIsoelectric Point Local Chemical Equilibrium Zone Width Zone Evolution Focus Zone
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