Abstract
High performance liquid chromatography (HPLC) has been established during the past 10 years as one of the primary methods for the separation and purification of carotenoids and retinoids. The rapid analysis times (10 to 45 min), high sensitivity (low ng), high resolving power, high recovery and non-destructive conditions of HPLC make it an ideal method for carotenoid and retinoid analysis.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
R.F. Taylor, Chromatography of carotenoids and retinoids, Adv. Chromatogr. 22: 157 (1983).
K. Tsukida, K. Saiki, T. Takii, and Y. Koyama, Separation and determination of cis/trans-ß-carotenes by high performance liquid chromatography, J. Chromatogr. 245:359 (1982).
M. Vecchi, E. Glinz, V. Meduna, and K. Schiedt, HPLC separation and determination of astacene, semiastacene, astaxanthin, and other keto-carotenoids, J. High Resol. Chrom. Comm. 10: 348 (1987).
Y.M. Peng, J. Beaudry, D.S. Alberts, and T.P. Davis, High performance liquid chromatography of the provitamin A ß-carotene in plasma, J. Chromatogr. 273:410, (1983).
W.J. Driskell, M.M. Bashor and J.W. Neese, Beta-carotene determined in serum by liquid chromatography with a internal standard, Clin. Chem. 29:1042 (1983).
N. Katrangi, L.A. Kaplan, and E.A. Stein, Separation and quantitation of serum ß-carotene and other carotenoids by high performance chromatography, J. Lipid Res. 25:400 (1984).
D.W. Nierenberg, Serum and plasma ß-carotene levels measured with an improved method of high-performance liquid chromatography, J. Chromatogr. 339:273 (1985).
K.W. Miller and C.S. Yang, An isocratic high-performance liquid chromatography method for the simultaneous analysis of plasma retinol, α-tocopherol, and various carotenoids, Anal. Biochem. 145:21 (1985).
D.B. Milne and J. Botnen, Retinol, α-tocopherol, lycopene and α- and ß-carotene simultaneously determined in plasma by isocratic LC. Clin. Chem. 32:874 (1986).
T. Maoka and T. Matsuno, the first isolation and identification of tunaxanthin D. from marine fishes, Bull. Jap. Soc. Sci. Fish. 51:1349 (1985).
Y. Ikuno, T. Maoka, M. Shimizu, T. Komori, and T. Matsuno, Direct diastereometric resolution of carotenoids II. All ten stereoisomers of tunaxanthin, J. Chromatogr. 328:387 (1985).
T. Maoka, T. Komori, and T. Matsuno, Direct diastereometric resolution of carotenoids I. 3-Hydroxy-4-oxo-ß-end group, J. Chromatogr. 318:122 (1985).
F.T. Gillan and R.B. Johns, Normal-phase HPLC analysis of microbial carotenoids and neutral lipids, J. Chromatogr. Sci. 21:34 (1983).
R.F.C. Mantoura and C.A. Llewellyn, The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high-performance chromatography, Anal. Chim. Acta. 151:297 (1983).
S.W. Wright and J.D. Shearer, Rapid extraction and HPLC of chlorophylls and carotenoids from marine phytoplankton, J. Chromatogr. 294:281 (1984).
H.J.C.F. Nells and A.P. De Leenheer, Isocratic nonaqueous reversed-phase liquid chromatography of carotenoids, Anal. Chem. 55:270 (1983).
O.H. Will III and M. Ruddat, C18 reversed-phase HPLC analysis of carotenoids, Liq. Chromatogr. 2:610 (1984).
O. Beyer, G. Weiss, and H. Kleinig, Solubilization and reconstitution of the membrane-bound carotenogenic enzymes from daffodil chromoplasts, Eur. J. Biochem. 153:341 (1985).
B.H. Chen and C.A. Bailey, Separation of carotenoids in turf bermuda grasses by HPLC, J. Chromatogr. 393:297 (1987).
P.S. Stuart, P.A. Bailey and J.L. Beven, HPLC separation of carotenoids in tobacco and their characterization with the aid of a microcomputer, J. Chromatogr. 282:589 (1983).
R.J. Bushway, Separation of carotenoids in fruits and vegetables by HPLC, J. Liq. Chromatogr. 8:1527 (1985).
R.J. Bushway, Determination of α-and ß-carotene in some raw fruits and vegetables by HPLC, J. Agric. Food Chem. 34:409 (1986).
S.J. Schwartz and M. Patroni-Killam, Detection of cis-trans carotene isomers by two-dimensional thin-layer and HPLC, J. Agric. Food Chem. 33:1160, 1985.
F. Khachik, G.R. Beecher and N.F. Whittaker, Separation, identification and quantification of the major carotenoid and chlorophyll constituents in extracts of several green vegetables by LC, J. Agric. Food Chem. 34:603 (1986).
C. Fisher and J.A. Kocis, Separation of paprika pigments by HPLC, J. Agric. Food Chem. 35:55 (1987).
S.H. Ashoor and M.J. Knox, Determination of vitamin A derivatives in fortified foods and commercial vitamin supplements by HPLC, J. Chromatogr. 409:419 (1987).
W. Gau, H.J. Ploschke and C. Wunsche, MS identification of xanthophyll fatty acid esters from marigold flowers obtained by HPLC and craig counter-current distribution, J. Chromatogr. 262:277 (1983).
F. Zonta, B. Stancher, and G.P. Marietta, Simultaneous HPLC analysis of free carotenoids and carotenoid esters, J. Chromatogr. 403:207 (1987).
T. Philip and T.S. Chen., Separation and quantitative analysis of some carotenoid fatty acid esters of fruits by liquid chromatography, J. Chromatogr. 435:113 (1988).
M. Ito, T. Yamane, and K. Tsukida, HPLC separation and NMR identification of stereoisomers of 5,8-furanoidal retinoid, J. Chromatogr. 253:113 (1982).
G.M. Landers and J.A. Olson, Statistical solvent optimization for the separation of geometric isomers of retinol by HPLC, J. Chromatogr. 29l:51 (1984).
F. Zonta and B. Stancher, HPLC of retinals, retinols and their dehydro homologues: Improvements in resolution and spectroscopic characterization of the stereoisomers. J. Chromatogr. 301:64 (1984).
D.W. Nierenberg, Determination of serum and plasma concentrations of retinol using HPLC, J. Chromatogr. 311:239 (1984).
A.T.R. Williams. Simultaneous determination of vitamin A and E by liquid chromatography with fluorescence detection, J. Chromatogr. 341:198 (1985).
A.J. Speek, Microdetermination of vitamin A in human plasma using HPLC with fluorescence detection, J. Chromatogr. 382:284 (1986).
Y.M. Peng, M.J. Xu, and D.S. Alberts, Analysis and stability of retinol in plasma, J. Natl. Cancer Inst. 78:95 (1987).
M.E. Cullum and M.H. Zile, Quantitation of biological retinoids by HPLC: Primary internal standardization using tritiated retinoids, Anal. Biochem. 153:23 (1986).
J.L. Napoli, Quantification of physiological levels of retinoic acid, Meth. Enzymol. 123:112 (1986).
M. Makino-Tasaka and T. Suzuki, Quantitative analysis of retinal and 3-dehydroretinal by HPLC, Meth. Enzymol. 123:53 (1986).
B. Stancher and F. Zonta, HPLC of the unsaponifiable from samples of marine and freshwater fish: Fractionation and identification of retinol and dehydroretinal isomers, J. Chromatogr. 287:353 (1984).
J.G. Besner, S. Meloche, R. Leclaire, P. Band, and S. Mailhot, HPLC of Ro 10–9359 (Tigason) and its metabolite Ro 10–1670 in human plasma, J. Chromatogr. 231:467 (1982).
U. Paravicini and A. Busslinger, Determination of etretinate and its main metabolite in human plasma using normal-phase HPLC, J. Chromatogr. Biomed. Appl. 267:359 (1983).
T.A. Hultin, R.G. Megta, and R.C. Moon, Simple HPLC method for the separation of retinoids including N-(4-hydroxyphenyl)-all-trans- retinamide, J. Chromatogr. Biomed. Appl. 341:187 (1985).
D.D. Bankson, R.M. Russell, and J.A. Sadowski, Determination of retinyl esters and retinol in serum or plasma by normal-phase liquid chromatography: Method and applications, Clin. Chem. 321:35 (1986).
C. Ross. Separation and quantitation of retinyl esters and retinol by HPLC, Meth Enzymol. 123:68 (1986).
P.V. Bhat and A. Lacroix, Separation and estimation of retinyl fatty acyl esters in tissues and normal rat by HPLC, J. Chromatogr. 272: 269 (1983).
L.R. Chaudhary and E.C. Nelson, Separation of vitamin A and retinyl esters by reversed-phase HPLC, J. Chromatogr. 294:466 (1984).
P.E. Farrow, R.F. Taylor, and L.M. Yelle, The characterization of carotenoids and retinoids by HPLC-MS-MS, Proc. 35th ASMS Conference on Mass Spectrometry and Allied Topics, p. 1084 (1987).
R.F. Taylor, C.R. Hinckley, D.M. Eklund, and I.G. Marenchic, Effects of saponification on carotenoids, Proc. 8th International Symposium on Carotenoids. p. 19 (1987).
F. Khachik, G.R. Beecher, J.T. Vanderslice, and G. Farrow, Chromatographic artifacts and peak distortion in separation of carotenoids by HPLC: Sample-solvent interactions, Proc. 8th International Symposium on Carotenoids, p. P21 (1987).
P.J. Arpino, Ten years of liquid chromatography-mass spectrometry, J. Chromatogr. 323:3 (1985).
B.L. Karger and P. Vouros, A chromatography perspective of HPLC-MS, J. Chromatogr. 323:13 (1985).
T.R. Covey, E.D. Lee, A.P. Bruins, and J.D. Henion, Liquid chromatography-mass spectrometry, Anal. Chem. 58:1451A (1986).
F. W., McLafferty, Ed., “Tandem Mass Spectrometry”, John Wiley & Sons, New York (1983).
J. V., Johnson, and R. A. Yost, Tandem Mass Spectrometry for Trace Analysis, Anal. Chem. 57:758A (1985).
M.E. Rose, Metastable ion Techniques for the Analysis of Carotenoid Isomers, In: “Carotenoid Chemistry and Biochemistry”, G. Britton and T.W. Goodwin, Eds., Pergamon, Oxford (1982).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Plenum Press, New York
About this chapter
Cite this chapter
Taylor, R.F., Farrow, P.E., Yelle, L.M., Harris, J.C., Marenchic, I.G. (1989). Advances in HPLC and HPLC-MS of Carotenoids and Retinoids. In: Krinsky, N.I., Mathews-Roth, M.M., Taylor, R.F. (eds) Carotenoids. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0849-2_6
Download citation
DOI: https://doi.org/10.1007/978-1-4613-0849-2_6
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-8113-9
Online ISBN: 978-1-4613-0849-2
eBook Packages: Springer Book Archive