Carotenoids pp 293-300 | Cite as

β-Carotene Prevention of 7,12-Dimethylbenz [A] Anthracene-Induced Transformation of Mouse Mammary Cells In Vitro

  • K. Manoharan
  • S. Som
  • M. Chatterjee
  • M. R. Banerjee


Epidemiological studies have suggested that β-carotene, a dietary vitamin A precursor, may influence reduced tumor incidence in human populations1. In contrast to the abundance of studies2,3 on the chemopreventive properties of retinoids (vitamin A analogues) little is known about the mode of action of β-carotene, the vitamin A precursor. Only a few preliminary reports based on rather indirect observations have indicated that a carrot rich diet or injections of β-carotene result in reduced tumor incidence in rats and mice treated with a carcinogenic chemicals and/or u.v. radiation4–6. These observations prompted the investigations of the chemopreventive role of β-carotene itself in an in vitro controlled environment, because in the animal the vitamin A precursor is metabolized to retinol.


Mammary Gland Singlet Oxygen Mammary Epithelial Cell Sister Chromatid Exchange Initiation Stage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



7,12-dimethylbenz [a] anthracene


nodule-like alveolar lesions


dimethyl sulfoxide


hyperplastic alveolar nodules


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    R. Peto, Doll, R., J.D. Buckley, M.B. Sporn, Can dietary beta- carotene materially reduce human cancer rates, Nature, 290: 201 (1981).CrossRefGoogle Scholar
  2. 2.
    M.B. Sporn, D.L. Newton, J.M. Smith, N. Acton, A.E. Jacobson, and A. Brossi, Retinoids and cancer prevention: the importance of the terminal group of the retinoid molecule in modifying activity and toxicity, in: Carcinogenesis: Identification and mechanisms of action, A.C. Griffin and C.R. Shaw, eds., Raven Press, New York (1979)Google Scholar
  3. 3.
    M.B. Sporn and A.B. Roberts, The role of retinoids in differentiation and carcinogenesis, Cancer Res. 43: 3034 (1983)Google Scholar
  4. 4.
    J.H. Epstein, Effects of beta-carotene on UV-induced cancer formation in the hairless mouse skin, Photochem. Photobiol. 25: 211 (1973)CrossRefGoogle Scholar
  5. 5.
    M.M. Mathews-Roth, Antitumor activity of β-carotene, cauthaxanthin and phytoene, Oncologv 39: 33 (1982)CrossRefGoogle Scholar
  6. 6.
    A.C. Dorogokupula, A.C., E.G. Troitzkaia, L.K. Adilgireieva, S.F. Postolnikov, and Z.P. Chekrygina, Effects of carotene on the development of induced tumors, Zdravookhr Kazkh 10: 32 (1981)Google Scholar
  7. 7.
    M.R. Banerjee, N. Ganguly, N.M. Mehta, A.P. Iyer and R. Ganguly, Functional differentiation and neoplastic transformation in an isolated whole mammary organ in vitro, in: “Cell Biology of Breast Cancer”, C. McGrath, M. Brennan and M. Rich, eds., Academic Press, NY, 485 (1980)Google Scholar
  8. 8.
    M.R. Banerjee, In Vitro model for neoplastic transformation mouse mammary epithelial cells in an isolated whole mammary organ in vitro, in: “In vitro models of Cancer Research”, M. Webber and Sekley, eds., CRC Press, Boca Raton, Florida (1986)Google Scholar
  9. 9.
    K.B. DeOme, L.T. Faulkin, Jr., H.A. Bern and P.B. Blair, Development of mammary tumors from hyperplastic alveolar nodule transplanted into gland-free mammary fat pad of female C3H mice, Cancer Res. 19: 515 (1959)Google Scholar
  10. 10.
    N.T. Telang, M.R. Banerjee, A.P. Iyer and A.B. Kundu, Neoplastic transformation of epithelial cells in whole mammary gland in vitro, Proc. Natl. Acad. Sci. U.S.A. 76:5886 (1979)CrossRefGoogle Scholar
  11. 11.
    A.P. Iyer and M.R. Banerjee, Sequential expression of preneoplastic and neoplastic characteristics of mouse mammary epithelial cells transformed in organ culture, J. Natl. Cancer Inst. 66: 893 (1981)Google Scholar
  12. 12.
    S. Som, M. Chatterjee and M.R. Banerjee, β-carotene inhibition of DMBA-induced transformation of murine mammary cells in vitro, Carcinogenesis 5: 937 (1984)CrossRefGoogle Scholar
  13. 13.
    D.S. Goodman and H.S. Huang, Biosynthesis of vitamin A with rat intestinal enzymes, Science 149: 879 (1965)CrossRefGoogle Scholar
  14. 14.
    C. Heidelberger, Chemical carcinogenesis, Ann. Rev. Biochem. 44:79 (1975)CrossRefGoogle Scholar
  15. 15.
    A.B. Kundu, N.T. Telang and M.R. Banerjee, The binding of 7, 12 dimethylbenz [a] anthracene to mammary cell DNA in organ culture, J. Nat. Cancer Inst. 61: 465 (1978)Google Scholar
  16. 16.
    G.W. Burton and K.V. Ingold, β-Carotene: An unusual type of lipid antioxidant, Science 224:569 (1984)CrossRefGoogle Scholar
  17. 17.
    C.S. Foote and R.W. Denney, Photosensitized oxidation and singlet oxygen: Consequence in biological system, J. Am. Chem. Soc. 90: 6233 (1968)CrossRefGoogle Scholar
  18. 18.
    K. Manoharan and M.R. Banerjee, Measurements of chemical carcinogen- induced sister chromatid exchanges in a whole organ in vitro, Mutation Res 147: 165 (1985)Google Scholar
  19. 19.
    K. Manoharan and M.R. Banerjee, β-Carotene reduces sister chromatid exchanges induced by chemical carcinogens in mouse mammary cells in organ culture, Cell Biol. Intern’t Reports 9:783 (1986)CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • K. Manoharan
    • 1
  • S. Som
    • 1
  • M. Chatterjee
    • 1
  • M. R. Banerjee
    • 1
  1. 1.Tumor Biology Laboratory, School of Biological SciencesUniversity of Nebraska-LincolnLincolnUSA

Personalised recommendations