Biotechnological Applications Of Reversed Micelles
Three emerging biotechnological applications of reversed micelles are described and discussed, viz, the bioconversions of apolar compounds, the recovery of extracellular enzymes by liquid-liquid extraction, and the isolation of intracellular enzymes from intact bacterial cells. For each application one example is given which is illustrative of that particular field. For bioconversions, the chiral reduction of α,β-unsaturated carboxylates was taken as an example, and for the second application the extraction of the extracellular enzyme α-amylase. The feasibility of reversed micelles as a bioseparation tool for intracellular proteins was tested by following the activity and recovery of β-hydroxybutyrate dehydrogenase, isocitrate dehydrogenase and glucose-6-phosphate dehydrogenase from the bacterium Azotobacter vinelandii.
KeywordsSurfactant NADH CTAB Amylase Lysozyme
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