De Novo Synthesis of Pregnancy-Specific and Pregnancy-Associated Proteins by the In Vitro Perfused Human Term Placenta
Placental perfusion is one of several in vitro techniques to investigate the production of pregnancy proteins by the trophoblast as well as their control mechanisms. The preparation as described in the chapter by Schneider et al is a closed-circuit system — that is, the released proteins and hormones accumulate in the circulating perfusate. This has the advantage of consuming less medium and yielding smaller volumes of more concentrated solutions when compared with the open perfusion protocol. We are aware that the release kinetics may differ between open and closed circuit protocols. For this reason we have abandoned our first protocol which required a full medium change after two hours of perfusion1 and have followed a new one which required the removal of 5 ml aliquots from both circuits every 30 minutes during the first two hours and every 60 minutes thereafter. The removed aliquots were replaced with fresh medium injected into both circulations.
KeywordsTissue Extract Fetal Circulation Maternal Circulation Label Amino Acid Iodoacetic Acid
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