Immunochemical Analysis of Histoplasmin Proteins and Polysaccharide

  • Errol Reiss
  • Sandra L. Bragg

Abstract

Histoplasmin is the supernatant fluid obtained after static cultivation of the mycelial form of Histoplasma capsulatum for 3 to 6 months at ambient temperature (22 to 25°C) on asparagine-glucose-glycerol-salts medium (Smith et al, 1948). Three types of histoplasmin exist — skin test reagents from the mycelial and yeast forms and serological histoplasmin. The method of preparing skin test histoplasmin was established long ago (Shaw et al, 1950) and single lots were used widely for many years. The skin test antigen was standardized by its ability to elicit a reproducible cutaneous hypersensitivity response in sensitized humans. The antigens in histoplasmin responsible for the skin test have been inferred but no direct structure-activity evidence exists, because monomolecular antigens have not been available. Recently, histoplasmin from yeast form cultures has been approved for human use as a skin test antigen (Scalarone et al, 1985). The bulk of accumulated information concerns mycelial histoplasmin and there are far fewer data about the characteristics of the yeast form product. For that reason, and because of space limitation, the following discussion applies primarily to mycelial histoplasmin.

Keywords

Cellulose Hydrolysis Carbohydrate Mold Polysaccharide 

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Errol Reiss
    • 1
  • Sandra L. Bragg
    • 1
  1. 1.Division of Mycotic Diseases, Center for Infectious Diseases, Centers for Disease Control, Public Health ServiceUS Department of Health and Human ServicesAtlantaUSA

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