Advertisement

Isolation And Biochemical Characterization Of The D1 And D2 Dopamine Receptors

  • Jay A. Gingrich
  • Susan E. Senogles
  • Nourdine Amilaiky
  • Wei K. Chang
  • Joel G. Berger
  • Marc G. Caron
Chapter
  • 91 Downloads

Abstract

The catecholamine, dopamine, exerts physiologic effects in both the central nervous system and the periphery. Dopamine systems in the central nervous system (CNS) have been implicated in several neurologic and psychiatric disorders such as Parkinsonism, schizophrenia, Huntington’s chorea, Tourette’s syndrome, and Lesch-Nyhan syndrome. In the periphery, dopamine plays a role in processes controlling renal vascular tone and release of the hormones, prolactin from the anterior pituitary gland and parathyroid hormone from the parathyroid gland. The symptoms of some pathological processes are ameliorated through the use of drugs targeted toward the receptors for dopamine. For example, dopamine agonists have been successfully used to treat Parkinson’s disease, hyperprolactinemia (prolactinomas), and to prevent renal ischemia associated with cardiovascular collapse (shock), and dopamine antagonists have proven useful in the treatment of schizophrenia, and Tourette’s syndrome.

Keywords

Dopamine Receptor Guanine Nucleotide Binding Protein Striatal Tissue Photoaffinity Labelling Renal Vascular Tone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Amlaiky, N. & Caron., M.G. (1985) Photoaffinity of the D2-dopamine receptor using a novel high affinity radio-iodinated probe. J. Biol. Chem. 260: 1983–1986.PubMedGoogle Scholar
  2. Amilaiky. N. Berger, J.G., Chang, W., McQuade, R.J., & Caron, M.G. (1987) Identification of the binding subunit of the D1-dopamine receptor by photoaffinity crosslinking. Mol. Pharmacol. 31: 129–134.Google Scholar
  3. Amlaiky, N. and Caron, M.G., (1986) Identification of the D2-dopamine receptor binding subunit in several mammalian tissues and species by photoaffinity labelling. J. Neurochem. 47: 196–204.PubMedCrossRefGoogle Scholar
  4. Arnt, J. (1987) Behavioral studies of dopamine receptors: evidence for regional selectivity and receptor multiplicity. in Dopamine Receptors, (Creese, I., & Fraiser, C.M., eds.) pp. 199–231, Alan R. Liss, New York.Google Scholar
  5. Barone, P., Davis, T.A., Braun, A.R., & Chase, T.N. (1986) Dopaminergic mechanisms and motor function: characterization of D-l and D-2 dopamine receptor interactions. Eur. J. Pharmacol. 123: 109–114.PubMedCrossRefGoogle Scholar
  6. Billard, W., Ruperto, V., Crosby, G., Iorio, L. C., & Barnett, A. (1984) Characterization of the binding of [3H]SCH 23390, a selective D-l receptor antagonist ligand in rat striatum. Life Sci. 35: 1885–1893.PubMedCrossRefGoogle Scholar
  7. Caron, M.G., Kilpatrick, B.F., and De Lean, A. (1983) The dopamine receptor of the anterior pituitary gland: Ligand binding and solubilization studies, in Dopamine Receptors (C. Kaiser and J.W. Kebabian, eds). ACS Symposium Series 224, pp. 73–92.CrossRefGoogle Scholar
  8. Christensen, A.V., Arnt, J., Hyttel, J., Larsen, J., & Svendsen, O. (1984) Pharmacological effects of specific dopamine D-l antagonist SCH 23390 in comparison with neuroleptics. Life Sci. 34: 1529–1540.PubMedCrossRefGoogle Scholar
  9. Clark, D., and White, F.J., (1987) Review: D1 dopamine receptor-The search for a function: A critical evaluation of the D1/D2 dopamine receptor classification and its functional implications. Synapse. 1:347–388.PubMedCrossRefGoogle Scholar
  10. DeLean, A.U., Kilpatrick B.R., & Caron, M.G. (1982) Dopamine receptor of the porcine anterior pituitary gland: Evidence for two affinity states discriminated by both agonists and antagonists. Mol. Pharmacol. 22:290–297.PubMedGoogle Scholar
  11. Enjalbert, A., Sladeczek, F., Guillon, G., Bertrand, P., Shu, C., Epelbaum, J., Garcia-Sainz, A., Jard, S., Lombard, C., Kordon, C., & Bockaert, J., (1986) Angiotensin II and dopamine modulate both cAMP and inositol phosphate productions in anterior pituitary cells. J. Biol. Chem. 261:4071–4075.PubMedGoogle Scholar
  12. Gershanik, O., Heikkila, R.E., & Duvoisin, R.C. (1983) Behavioral correlations of dopamine receptor activation. Neurology. 33:1489–1492.PubMedGoogle Scholar
  13. Gingrich, J.A., Amlaiky, N., Senogles, S.E., Chang, W.K., McQuade, R.D., Berger, J.G., Caron, M.G., (1988) Affinity chromatography of the D1 dopamine receptor from rat corpus striatum, FASEB abstract.Google Scholar
  14. Iorio, L.C., Barnett, A., Leitz, F.H., Houser, V.P., & Korduba, C. A. (1983) SCH 23390, apotential benzazepine antipsychotic with unique interactions on dopaminergic systems. J. Pharmacol, and Exp. Therap. 226:63–468.Google Scholar
  15. Kebabian, J.W. & Calne, D.B. (1979) Multiple receptors for dopamine. Nature 277:93–96.PubMedCrossRefGoogle Scholar
  16. Kebabian, J.W. & Greengard, P. (1971) Dopamine-sensitive adenyl cyclase: possible role in synaptic transmission. Science 174:1346–1349.PubMedCrossRefGoogle Scholar
  17. Kilpatrick, B.F. and Caron, M.G. (1983) Agonist binding promotes a guanine nucleotide reversible increase in the apparent size of the bovine anterior pituitary dopamine receptor. J. Biol, chem 258:13528–13534.PubMedGoogle Scholar
  18. Kilpatrick, B.F. and Caron, I. (1984) Dopamine receptor of the porcine anterior pituitary band: Solubilization and characterization. Biochem. Fkrmacol. 33:1981–1988.CrossRefGoogle Scholar
  19. Leff, S.E., & Creese, I. (1985b) Interactions of dopaminergic agonists and antagonists with dopaminergic D3 binding sites in rat striatum. Mol. Pharmacol. 27:184–192PubMedGoogle Scholar
  20. Margaroli, A., Vallai, L., Elaki, F.R., Pozzan, T., Spada, A., and Meldolesi, J. (1987) Dopamine inhibits cytosolic Ca+ + increases in rat lactotroph cells: Evidence of a dual mechanism of action. J. Bil. Chem. 262:1390–13927.Google Scholar
  21. Robertson, G.S. & Robertson, H.A. (1986) Synergistic effects of D1 and D2 dopamine agonists on turning behavior in rats. Eur. J. Pharmacol. 384:387–390.Google Scholar
  22. Sasaki, K. & Soto, M. (1987) A single GTP-binding protein regulated K + -channels coupled with dopamine, histamine and acetylcholine receptors. Nature. 325:259–262.PubMedCrossRefGoogle Scholar
  23. Seeman, P. (1980) Brian dopamine receptors. Pharm. Rev. 32:229–313.PubMedGoogle Scholar
  24. Senogles, S.E., Amlaiky, N., Johnson, A.L., & Caron, M.G. (1986) Affinity chromotography of the anterior pituitary D2-dopamine receptor. Biochemistry 25:749–753.PubMedCrossRefGoogle Scholar
  25. Senogles, S.E., Benovic, J.L., Amlaiky, N., Unson, C., Milligan, G., Vinitksy, R., Spiegel, A., and Caron, M.G., (1987) The D2 dopamine receptor of anterior pituitary is functionally associated with a pertussis toxin-sensitive guanine nucleotide binding protein. J. Biol. Chem. 262:3106–3113.Google Scholar
  26. Senogles, S.E., Amlaiky, N., & Caron, M.G., (1988) Purification of the D2 dopamine receptor from anterior pituitary. FASEB abstract.Google Scholar
  27. Stoff, J.C. & Kebabian, J.W. (1984) Two domapine receptors: biochemistry and pharmacology. Life Sci, 35:2281–2296.CrossRefGoogle Scholar
  28. Waddington, J.L. (1986) Behavioral correlates of the action of selective D-l dopamine receptor antagonists. Biochem. Pharmacol. 35:3661–3667.PubMedCrossRefGoogle Scholar
  29. Walters, J.R., Bergstrom, D.A., Carlson, J.H., Chase, T., & Braun, A.L. (1987) D1 Dopamine receptor activation required for postsynaptic expression of D2 agonist effects Science 236:719–722.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Jay A. Gingrich
    • 1
  • Susan E. Senogles
    • 1
  • Nourdine Amilaiky
    • 1
  • Wei K. Chang
    • 2
  • Joel G. Berger
    • 2
  • Marc G. Caron
    • 1
  1. 1.Departments of Physiology Medicine, Howard Hughes Medical Institute LaboratoriesDuke University Medical CenterDurhamUSA
  2. 2.Research DivisionSchering-Plough CorpBloomfieldUSA

Personalised recommendations