Hydrogen Peroxide Production in Dopamine Neurons: Implications for Understanding Parkinson’s Disease

  • Gerald Cohen
  • Mary Beth Spina


The turnover of monoamine neurotransmitters in brain should, in theory, be associated with an increased flux of hydrogen peroxide. The H202 arises as a result of the oxidative deamination of the neurotransmitters by monoamine oxidase (MAO).


Dopamine Neuron Oxidize Glutathione Oxidative Deamination Monoamine Neurotransmitter Glutathione Disulfide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Adams, J.D., Lauterberg, B.H., and Mitchell, J.R., Plasma glutathione and glutathione disulfide in the rat: regulation and response to oxidative stress. J. Pharmacol. Exp. Ther. 227: 749–754, 1983.PubMedGoogle Scholar
  2. Altar, C.A., Marien, M.R., and Marshall, J.E, Time course of adaptations in dopamine biosynthesis, metabolism, and release following nigrostriatal lesions: implications for behavioral recovery from brain injury. J. Neurochem. 48: 390–399, 1987.PubMedCrossRefGoogle Scholar
  3. Cohen, G., The pathobiology of Parkinson’s disease: biochemical aspects of dopamine neuron senescence. J. Neural Trans. Suppl. 19: 89–103, 1983.Google Scholar
  4. Gilbert, H.F., Biological disulfides: the third messenger? J. Biol. Chem. 257: 12086–12091, 1982.PubMedGoogle Scholar
  5. Hefti, F., Melamed, E., and Wurtman, R.J., Partial lesions of the dopaminergic nigrostriatal system in the rat brain: Biochemical characteristics. Brain Res. 195: 123–137, 1980.PubMedCrossRefGoogle Scholar
  6. Hefti, F., Melamed, E., and Wurtman, R.J., The site of dopamine formation in rat striatum after L-dopa administration. J. Pharmacol. Exp. Ther. 217: 18–197, 1981.Google Scholar
  7. Hornykiewicz, O. and Kish, S.J., Biochemical pathophysiology of Parkinson’s disease. In Advances in Neurology, 45: 19–34, 1986.Google Scholar
  8. Maker, H.S., Weiss, C., Silides, D.J., and Cohen, G., Coupling of dopamine oxidation (monoamine oxidase activity) to glutathione oxidation via the generation of hydrogen peroxide in rat brain homogenates. J. Neurochem. 36: 589–593, 1981.PubMedCrossRefGoogle Scholar
  9. Perry, T.L., Godin, D.V., and Hansen, S., Parkinson’s disease: a disorder due to nigral glutathione deficiency? Neurosi. Lett. 33: 305–310 (1982).CrossRefGoogle Scholar
  10. Oshino, N. and Chance, B., Properties of glutathione release observed during reduction of organic hydroperoxide, demethylation of aminopyrine and oxidation of some substances in perfused rat liver, and their implications for the physiological function of catalase. Biochem. J. 162: 509–525, 1977.PubMedGoogle Scholar
  11. Sies, H., Hydroperoxides and thiol oxidants in the study of oxidative stress in intact cells and organs. In H. Sies (Ed.), Oxidative Stress, Academic, London, 73–90, 1985.Google Scholar
  12. Sinet, P.M., Heikkila, R.E., and Cohen, G., Hydrogen peroxide production by rat brain in vivo. J. Neurochem. 34: 1421–1428, 1980.PubMedCrossRefGoogle Scholar
  13. Slivka, A., Spina, M.B., and Cohen, G., Reduced and oxidized glutathione in human and monkey brain. Neurosci. Lett. 74: 112–118, 1987.PubMedCrossRefGoogle Scholar
  14. Spina, M.B. and Cohen, G., Exposure of striatal synaptosomes to L-Dopa increases levels of oxidized glutathione, Trans. Amer. Soc. Neurochem., in press, 1988.Google Scholar
  15. Tietze, F., Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Analyt. Biochem. 27: 502–522, 1969.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Gerald Cohen
    • 1
  • Mary Beth Spina
    • 1
  1. 1.Department of Neurology, Neurobiology Center and Graduate School of Biological SciencesMount Sinai School of Medicine of the City University of New YorkNew YorkUSA

Personalised recommendations