Abstract
The synthesis of immunoglobulin light and heavy chains is encoded by genes, each of which contains coding exons and sequences that carry out gene regulation in the cell. The immunoglobulin genes of some plasmacytoma cell lines have been studied in detail (Kabat et al., 1983). The structure of genes from mature lymphocytes that synthesize antibodies of a definite specificity are of great interest. The isolation of such genes and sequencing their structure allows one to establish the amino acid sequence of antibody polypeptide chains as well as the location and arrangement of regulatory sequences that exist in the promoter and enhancer regions of mature lymphocytes. This is a necessary step in studying the regulation of expression of genes coding for the light and heavy chains of antibodies. This study was made with habridoma PTF.02 characterized by a stable synthesis and a high yield of antibodies against pig transferrin (Bartek et al., 1982). This transferrin is known now to be an important cell growth factor and is used for the cultivation of myeloma and hybridoma cells in serum-free media.
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© 1988 Plenum Press, New York
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Deyev, S.M., Ajalov, V.A., Urackov, D.N., Stepchenko, A.G., Franĕk, F., Polyanovsky, O.L. (1988). Investigation of Immunoglobulin Light and Heavy Chain Genes Responsible for the Synthesis of Antibodies in Hybridoma PTF.02. In: Zelinka, J., Balan, J. (eds) Metabolism and Enzymology of Nucleic Acids. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0749-5_39
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DOI: https://doi.org/10.1007/978-1-4613-0749-5_39
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