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Crystallization and Preliminary X-Ray Structural Studies of RNase Th1 from Trichoderma Harzianum

  • K. M. Polyakov
  • B. V. Strokopytov
  • A. A. Vagin
  • S. I. Bezborodova
  • S. V. Shlyapnikov

Abstract

RNase Th1 is an alkaline guanyl-specific fungal ribonuclease. Primary structure of RNase Th1 has been determined (Table 1). The homology of RNase Th1 with other fungal RNases (T1, C2, Pb1) is about 70%.

Keywords

Ammonium Sulphate Asymmetric Unit Equilibrium Dialysis TRICHODERMA HARZIANUM Molecular Replacement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Stokopytov, B.V. 1987, “Kristallografiya” (USSR), in press.Google Scholar
  2. Sussman, J.L., Holbrook, S.R., Church, G.M., Kim, S.H. 1977, Acta Cryst., A33; 80.CrossRefGoogle Scholar
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  4. Growther, R.A. 1971, in: “Molecular Replacement Method,” ed., Rossman, Gordon and Breach, N.Y.Google Scholar
  5. Vagin, A.A. 1983, Ph.D. Thesis Institute of Crystallography.Google Scholar
  6. Polyakov, K.M., Strokopytov, B.B., Vagin, A.A., Tishenko, G.N., Bezborodova, S.I., Vainshtein, B.K. 1987, Kristallografiya. USSR, 32:3Google Scholar
  7. Borisova, S.N., Vagin, A.A., Nekrasov, J.V., Pavlovsky, A.G., et al. 1986, Kristallografiya USSR, 31; 474.Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • K. M. Polyakov
    • 1
  • B. V. Strokopytov
    • 1
  • A. A. Vagin
    • 1
  • S. I. Bezborodova
    • 2
  • S. V. Shlyapnikov
    • 3
  1. 1.Academy of Sciences of the USSRInstitute of CrystallographyMoscowRussia
  2. 2.VNII “Genetika”MoscowRussia
  3. 3.Academy of Sciences of the USSRInstitute of Molecular biologyMoscowUSSR

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