Abstract
The tertiary structure of ribonuclease Sa from Streptomyces aureofaciens as well as the structure of its complex with 3’guanylic acid (3’GMP) have been determined by X-ray analysis. The electron density that was finally interpreted was calculated with phases obtained by multiple isomorphous replacement technique and solvent flattening at a resolution of 2.5 A. Refinement against 1.8 A data yields R factor of 0.172 and 0.184 for the native and complex structures, respectively. The three dimensional structure of the enzyme is similar to that of other microbial ribonucleases and, as expected from the amino acid sequences, is closest to RNase St. For comparison sequences of ribonucleases T1 (Takahashi, 1980), C2 (Bezborodova, 1983), Pchl (Shlyapnikov, 1986), Pbl (Shlyapnikov, 1984), Ba (Hartley, 1972), Bi (Aphanasenko, 1980), St (Yoshida, 1976), and Sa (Shlyapnikov, 1986) are given in Table 1.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Aphanasenko, G. A., Dudkin, S. M., Kaminir, L. B., Leshchinskaya, I. A., and Severin, E. S., 1980, FEBS Lett., 97:77–80.
Bezborodova, S. I., Khodova, O. M., and Stepanov, V. M., 1983, FEBS Lett., 159:256–257.
Blow, D. M., and Crick, F. H., 1959, Acta Cryst., 12:794–802.
Dodson, E. J., 1975, in:•Crystallographic Computing Techniques•, F. R. Ahmed, ed., Munskgaard, Copenhagen, 260–268.
Gasperik, J., Frescakova, S., and Zelinka, J., 1982, Biologia, Bratislava, 37:377–381.
Hartley, R. W., and Baker, E. A., 1972, Nature, 235:15–16.
Hill, C., Dodson, G., Heineman, V., Seanger, W., Mitsui, Y., Nakamura, K., Borisov, S., Tischenko, G., and Polyakov, S., 1983, TIBS, 8:366–369.
Jones, T. A., 1978, J. App. Crystallogr., 11:268–272.
Konnert, J. H., and Hendricson, W. A., 1980, Acta Cryst., A36:344–350.
Sevcik, J., Gasperik, J., and Zelinka, J., 1982, Gen. Phisiol. Biophys., 1:255–259.
Shlyapnikov, S. V., Kulikov, V. A., and Yakovlev, G. I., 1984, FEBS Lett., 177:246–248.
Shlyapnikov, S. V., Bezborodova, S. I., Kulikov, V. A., and Yakovlev, G. I., 1986, FEBS Lett., 196:29–33.
Shlyapnikov, S.V., Both, V., Kulikov, V.A., Dementiev, A.A., Sevcik, J., Zelinka, J. 1986 FEBS Lett., 209:335–339.
Takahashi, K. J., 1971, Biochem., Tokyo, 70:945–960.
Wang, B. C., 1985, in: “Methods in Enzymology”, H. W. Wyckoff, C. H. Hirs, and S. N. Timasheff, eds., 114:90–112.
Wilson, K. S., 1978, Acta Cryst., B34:1599–1608.
Yoshida, N., Sasaki, A., Rashid, M. A., and Otsuka, M., 1976, FEBS Lett., 64:122–125.
Zelinkova, E., Bacova, M., and Zelinka, J., 1971, Biochem. Biophys. Acta, 235:343–352.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Plenum Press, New York
About this chapter
Cite this chapter
Ševčik, J., Dodson, E.J., Dodson, G.G., Zelinka, J. (1988). The X-Ray Analysis of Ribonuclease Sa. In: Zelinka, J., Balan, J. (eds) Metabolism and Enzymology of Nucleic Acids. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0749-5_2
Download citation
DOI: https://doi.org/10.1007/978-1-4613-0749-5_2
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-8063-7
Online ISBN: 978-1-4613-0749-5
eBook Packages: Springer Book Archive